Advances in Micropropagation of a Highly Important Cassia species- A Review

Anis, Mohammad; Siddique, Iram; Naz, Ruphi; Ahmed, M. Rafique; Aref, Ibrahim M.

doi:10.5772/38942

Cited by 8 publications

(5 citation statements)

References 63 publications

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“…Moreover, the inevitable ruthless exploitation of this plant leading to their depletion from the wild is a cause for concern. In vitro propagation of plants holds tremendous potential for mass propagation and improvement with the production of high-quality plant-based medicines [7,8]. Owing to the immense importance of the plant, many authors have reported regeneration in W. somnifera using various explants [9][10][11][12][13][14][15], but none of the reports provide a comprehensive and detailed study on its regeneration systems which could apprehend the problems faced by the plant.…”

Section: Introductionmentioning

confidence: 99%

Interactive Effects of Growth Regulators, Carbon Sources, pH on Plant Regeneration and Assessment of Genetic Fidelity Using Single Primer Amplification Reaction (SPARS) Techniques in Withania somnifera L.

Fatima

Ahmad

et al. 2015

Appl Biochem Biotechnol

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An improved and methodical in vitro shoot morphogenic approach through axillary bud multiplication was established in a drug yielding plant, Withania somnifera L. Effects of plant growth regulators [6-benzyladenine (BA), kinetin (Kin), 2-isopentenyladenine (2iP), and thidiazuron (TDZ)] either singly or in combination with α-napthalene acetic acid (NAA), indole-3-butyric acid (IBA), and indole-3-acetic acid (IAA) in Murashige and Skoog (MS) medium were tested. The highest regeneration frequency (90 %) with optimum number of shoots (32 ± 0.00)/explant were obtained on MS medium fortified with 2.5 μM 6-benzyladenine (BA) and 0.5 μM NAA and 30 g/l sucrose at pH 5.8. Among the tried TDZ concentrations, 0.5 μM resulted in maximum number of shoots (20.4 ± 0.40)/explant after 4 weeks of exposure. The proliferating shoot cultures established by repeated subculturing of the mother explants on the hormone-free medium produced the highest shoot number (29.4 ± 0.40) with shoot length (6.80 ± 0.12 cm)/explant at fourth subculture passage, which a decline in shoot proliferation was recorded. Different concentrations of NAA were tested for ex vitro rooting of microshoots. The maximum percentage of rooting 100 % with maximum roots (18.3 ± 0.1) was achieved in soilrite when basal portion of the microshoots were treated with 200 μM (NAA) for 15 min per shoot. The plantlets went through hardening phase in a growth chamber, prior to ex vitro transfer. The PCR-based single primer amplification reaction (SPAR) methods which include random amplified polymorphic DNA (RAPD) and direct amplification of minisatellite DNA (DAMD) markers has been used for assessment of genetic stability of micropropagated plantlets. No variation was observed in DNA fingerprinting patterns among the micropropagated and the donor plants illustrating their genetic uniformity.

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Section: Introductionmentioning

confidence: 99%

Interactive Effects of Growth Regulators, Carbon Sources, pH on Plant Regeneration and Assessment of Genetic Fidelity Using Single Primer Amplification Reaction (SPARS) Techniques in Withania somnifera L.

Fatima

Ahmad

et al. 2015

Appl Biochem Biotechnol

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“…Where, other propagation techniques potentially hasten plant's large-scale proliferation, development, and conservation, tissue culture techniques made it possible to quickly produce large quantities of plants [13] . In vitro propagation is a biotechnological technique that is useful for the mass reproduction of medicinally important plants [108,109,110] , including cryopreservation and genetic alteration [111] . Hence, In vitro propagation techniques can readily be used to mass-proliferate V. peduncularis which is yet to be explored for its conservation purposes.…”

Section: Ethno-pharmacological Importance: Several Species Inmentioning

confidence: 99%

Importance of conserving Vitex peduncularis: A depleting forest resource with immense ethnopharmacological uses

Kumari.¹,

Sinha²,

Mishra³

2023

Pharma Innovation

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Vitex peduncularis, vernacularly known as 'Charaigorwa' is a large evergreen tree having numerous ethnopharmacological uses and belongs to the family Lamiaceae. The species is globally known for its usage to cure multifarious human health issues in traditional as well as contemporary medicines. The species exerts high bioactivity due to the presence of several secondary metabolites, including flavonoids, Vitexin, iridoid glycosides, pachypodol, ursolic acid, 2-hydroxy-ursolic acid terpenoids. Due to the high demands of V. peduncularis in pharmaceutical industries, the species is being exploited indiscriminately, pushing it towards the abyss of extinction. Immediate conservation measures are required to sustain the available natural population through multidisciplinary scientific interventions. This article cumulates the importance of the species directing towards its specialized pharmacological characteristics and its therapeutic uses. It also highlights the research gaps needed to be filled up for judicial use of the species conserving its depleting genetic resources.

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“…tora cultivados en medios de iniciación (gama de auxinas y/o BA desde 1x10 -3 hasta 0,01 mg L -1 ) para la micropropagación y regeneración de dichas especies, se evidenció una alta proliferación de plantas in vitro, en los medios que se habían empleado BA a concentraciones de 5x10 -4 a 0,01 mg L -1 , sin combinación de otros reguladores de crecimiento (Anis et al 2012). Agrawal y Sardar (2003), encontraron en plantas propagadas in vitro de Cassia sp., que al aumentar las concentraciones de BA hasta 0,01 mg L -1 había una tendencia a la disminución del porcentaje de explantes respondiendo al proceso de multiplicación, y del número promedio de brotes por explante.…”

Section: Efecto De La Concentración Del Ba Sobre El Crecimiento De Lunclassified

Estudios en la germinación y propagación in vitro de tres especies de Leguminosae: Calopogonium sp.,Stylosantes capitata Y Cassia moschata.

et al. 2020

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Calopogonium sp., Stylosanthes capitata y Cassia moschata, leguminosas de importancia en los ecosistemas de sabanas, presentan limitaciones en su cultivo debido a la latencia de sus semillas. En este trabajo se analizó la germinación de las semillas en cámara húmeda, y en condiciones in vitro, cultivándolas en medio Murashige y Skoog (1962). Las semillas previamente se sometieron a escarificación con etanol (Calopogonium sp.), e inmersión en ácido sulfúrico (C. moschata y S. capitata). En cámara húmeda, los valores de germinación fueron: Calopogonium sp., 60%, C. moschata 40%, de S. capitata 8%. La germinación in vitro, a los 21 días de cultivo para Calopogonium sp., fue del 100%, para S. capitata de 85% y para C. moschata de 65%. Para establecer una propagación masiva, se cultivaron in vitro, microesquejes aislados de plántulas obtenidas por germinación. Se analizaron tres medios de multiplicación constituidos por medios MS (1962) suplementados con benciladenina (0,5; 1; 1,5 mg L-1), denominados MS1, MS2 y MS3, respectivamente. Calopogonium sp., en MS2 y MS3 presentó formación y multiplicación de brotes, siendo mayor en MS3 (5 brotes/explante). C. moschata formó brotes en MS3 (1,66 brotes/explante). S. capitata formó brotes en los tres medios, alcanzando 7,5 brotes/explantes en el MS3

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Advances in Micropropagation of a Highly Important Cassia species- A Review

Cited by 8 publications

References 63 publications

Interactive Effects of Growth Regulators, Carbon Sources, pH on Plant Regeneration and Assessment of Genetic Fidelity Using Single Primer Amplification Reaction (SPARS) Techniques in Withania somnifera L.

Interactive Effects of Growth Regulators, Carbon Sources, pH on Plant Regeneration and Assessment of Genetic Fidelity Using Single Primer Amplification Reaction (SPARS) Techniques in Withania somnifera L.

Importance of conserving Vitex peduncularis: A depleting forest resource with immense ethnopharmacological uses

Estudios en la germinación y propagación in vitro de tres especies de Leguminosae: Calopogonium sp.,Stylosantes capitata Y Cassia moschata.

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