Advances in microfluidic strategies for single-cell research

Liu, Yixin; Fan, Zihui; Qiao, Liang; Liu, Baohong

doi:10.1016/j.trac.2022.116822

Cited by 19 publications

(12 citation statements)

References 193 publications

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“…This device is designed in a manner that allows individual cells to be effectively trapped in a mechanical barrier by the vortices induced by a fluid flow. 7 , 46 When a cell occupies a typical trap, the altered dynamic flow surrounding it has increased fluid resistance, which limits the access of other cells to the trap. Carlo et al.…”

Section: Single-cell Isolation Based On Microfluidicsmentioning

confidence: 99%

“…In recent years, a number of great reviews have been published to describe the combination of microfluidics and SCS techniques. 4 , 5 , 6 , 7 , 8 , 9 , 10 Here, we aim to provide a thorough description of single-cell isolation techniques based on different microfluidics design principles, as well as an overview of the complementary sequencing or profiling technologies used with these. We also highlight recent applications in precision oncology research and challenges and perspective of microfluidics-based SCS in clinical settings.…”

Section: Introductionmentioning

confidence: 99%

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Microfluidic design in single-cell sequencing and application to cancer precision medicine

Han,

Xu,

Yang

et al. 2023

Cell Reports Methods

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Section: Single-cell Isolation Based On Microfluidicsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Microfluidic design in single-cell sequencing and application to cancer precision medicine

Han,

Xu,

Yang

et al. 2023

Cell Reports Methods

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“…There are aptamer and antibody-based isolation methods for affinity-based isolation. The micro/nanofluidic systems are integrated with a variety of detection methods after successful isolation, including, colorimetry, fluorescence, electrochemistry, and surface-enhanced Raman scattering ( Gao et al, 2019b ; Liu et al, 2022 ).…”

Section: Nanotechnology Approaches For Diagnosis Of Ocmentioning

confidence: 99%

New trends in diagnosing and treating ovarian cancer using nanotechnology

Zhang

Ding

Zhang

et al. 2023

Front. Bioeng. Biotechnol.

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Ovarian cancer stands as the fifth most prevalent cancer among women, causing more mortalities than any other disease of the female reproductive system. There are numerous histological subtypes of ovarian cancer, each of which has distinct clinical characteristics, risk factors, cell origins, molecular compositions, and therapeutic options. Typically, it is identified at a late stage, and there is no efficient screening method. Standard therapies for newly diagnosed cancer are cytoreductive surgery and platinum-based chemotherapy. The difficulties of traditional therapeutic procedures encourage researchers to search for other approaches, such as nanotechnology. Due to the unique characteristics of matter at the nanoscale, nanomedicine has emerged as a potent tool for creating novel drug carriers that are more effective and have fewer adverse effects than traditional treatments. Nanocarriers including liposomes, dendrimers, polymer nanoparticles, and polymer micelles have unique properties in surface chemistry, morphology, and mechanism of action that can distinguish between malignant and normal cells, paving the way for targeted drug delivery. In contrast to their non-functionalized counterparts, the development of functionalized nano-formulations with specific ligands permits selective targeting of ovarian cancers and ultimately increases the therapeutic potential. This review focuses on the application of various nanomaterials to the treatment and diagnosis of ovarian cancer, their advantages over conventional treatment methods, and the effective role of controlled drug delivery systems in the therapy of ovarian cancer.

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“…In Figure 3d, the deformation ratio (%) in the x-axis represents the ratio of the deformation of the bead to its diameter. The orange curve shows the theoretical values using Equation (1). The blue dots represent the deformation of the PDMS beads measured using the microfluidic chip, and the corresponding reactive force was calculated using Equation ( 2), and the k was adjusted to fit the theoretical curves.…”

Section: Compression Concept Force Sensor Calibration and Stability O...mentioning

confidence: 99%

Integration of Microfluidic Chip and Probe with a Dual Pump System for Measurement of Single Cells Transient Response

Kaneko

Maruyama

et al. 2023

Micromachines

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The integration of liquid exchange and microfluidic chips plays a critical role in the biomedical and biophysical fields as it enables the control of the extracellular environment and allows for the simultaneous stimulation and detection of single cells. In this study, we present a novel approach for measuring the transient response of single cells using a system integrated with a microfluidic chip and a probe with a dual pump. The system was composed of a probe with a dual pump system, a microfluidic chip, optical tweezers, an external manipulator, an external piezo actuator, etc. Particularly, we incorporated the probe with the dual pump to allow for high-speed liquid change, and the localized flow control enabled a low disturbance contact force detection of single cells on the chip. Using this system, we measured the transient response of the cell swelling against the osmotic shock with a very fine time resolution. To demonstrate the concept, we first designed the double-barreled pipette, which was assembled with two piezo pumps to achieve a probe with the dual pump system, allowing for simultaneous liquid injection and suction. The microfluidic chip with on-chip probes was fabricated, and the integrated force sensor was calibrated. Second, we characterized the performance of the probe with the dual pump system, and the effect of the analysis position and area of the liquid exchange time was investigated. In addition, we optimized the applied injection voltage to achieve a complete concentration change, and the average liquid exchange time was achieved at approximately 3.33 ms. Finally, we demonstrated that the force sensor was only subjected to minor disturbances during the liquid exchange. This system was utilized to measure the deformation and the reactive force of Synechocystis sp. strain PCC 6803 in osmotic shock, with an average response time of approximately 16.33 ms. This system reveals the transient response of compressed single cells under millisecond osmotic shock which has the potential to characterize the accurate physiological function of ion channels.

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Advances in microfluidic strategies for single-cell research

Cited by 19 publications

References 193 publications

Microfluidic design in single-cell sequencing and application to cancer precision medicine

Microfluidic design in single-cell sequencing and application to cancer precision medicine

New trends in diagnosing and treating ovarian cancer using nanotechnology

Integration of Microfluidic Chip and Probe with a Dual Pump System for Measurement of Single Cells Transient Response

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