This study reports the development of a textile-based
colaminar
flow hybrid microbial–enzymatic biofuel cell. Shewanella MR-1 was used as a biocatalyst on the anode, and bienzymatic system
catalysts based on glucose oxidase and horseradish peroxidase were
applied on an air-breathing cathode to address the overpotential loss
in a body-friendly way. A single-layer Y-shaped channel configuration
with a double-inlet was adopted. Microchannels of biofuel cells were
patterned by silk screen printing with Ecoflex to maintain the flexibility
of textile substrates without harm to the human body. The electrodes
were fabricated with poly(3,4-ethylenedioxythiophene):polystyrene
sulfonate and a mixture of multiwalled carbon nanotubes and single-walled
carbon nanotubes by screen printing. The effects of electrode materials,
catalyst type, catalyst concentration, and glucose concentration in
the catholyte were investigated to optimize the fuel cell performance.
The peak power density (44.9 μW cm–2) and
maximum current density (388.9 μA cm–2) of
the optimized hybrid biofuel cell were better than those of previously
reported textile- or paper-substrate microscale single microbial fuel
cells. The developed biofuel cell will be a useful platform as a microscale
power source that is harmless to the environment and living organisms.