Advances in methods for colour marking of mosquitoes

Verhulst, Niels O.; Loonen, Jeanine; Takken, Willem

doi:10.1186/1756-3305-6-200

Cited by 60 publications

(62 citation statements)

References 28 publications

(35 reference statements)

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“…arabiensis aged 3–7 days that had no prior access to a bloodmeal were randomly aspirated from the cages and transferred to small holding cups 10 h before being used in semi‐field experiments. The two species were colour‐marked with either green or pink fluorescent powder [FTX Series, Astral Pink; Swada (London) Ltd, London, U.K.] to distinguish them after simultaneous release (Verhulst et al ., ). Mosquitoes were provided with water on cottonwool placed on top of the mosquito holding cups until their use in experiments.…”

Section: Methodsmentioning

confidence: 97%

Variation in host preferences of malaria mosquitoes is mediated by skin bacterial volatiles

Busula

Takken

Boer

et al. 2017

Medical Vet Entomology

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Abstract. The host preferences of the anthropophilic mosquito species in the Anopheles gambiae complex (Diptera: Culicidae) are mediated by skin bacterial volatiles. However, it is not known whether these mosquitoes respond differentially to skin bacterial volatiles from non-human host species. In this study, the responses of two malaria mosquito species in the An. gambiae complex, Anopheles gambiae s.s. (hereafter, An. gambiae) and Anopheles arabiensis, with different host preferences, to volatiles released from skin bacteria were tested. Skin bacteria collected from human, cow and chicken skin significantly increased trap catches; traps containing bacteria collected from human skin caught the highest proportions of An. gambiae and An. arabiensis. Traps with bacteria of human origin caught a significantly higher proportion of An. gambiae than of An. arabiensis, whereas bacterial volatiles from the chicken attracted significantly higher numbers of An. arabiensis than of An. gambiae. Additionally, An. gambiae showed a specialized response to volatiles from four specific bacteria, whereas An. arabiensis responded equally to all species of bacteria tested. Skin bacterial volatiles may therefore play important roles in guiding mosquitoes with different host preferences. The identification of these bacterial volatiles can contribute to the development of an odour blend that attracts mosquitoes with different host preferences.

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Section: Methodsmentioning

confidence: 97%

Variation in host preferences of malaria mosquitoes is mediated by skin bacterial volatiles

Busula

Takken

Boer

et al. 2017

Medical Vet Entomology

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“…Various methods for marking mosquitoes are available, including tags, dye, dust, (radioactive) isotopes and proteins. In general, such methods have little adverse effect on the released organisms, although careful consideration should be given not only to the direct impact on survival, but also on (host-seeking) behaviours (92). A major limitation of such studies is often the very low recapture rate.…”

Section: Short-range Dispersalmentioning

confidence: 99%

Mosquitoes and Culicoides biting midges: vector range and the influence of climate change

Elbers¹,

Koenraadt²,

Meiswinkel³

2015

Rev. Sci. Tech. OIE

109

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SummaryVector-borne animal diseases pose a continuous and substantial threat to livestock economies around the globe. Increasing international travel, the globalisation of trade, and climate change are likely to play a progressively more important role in the introduction, establishment and spread of arthropod-borne pathogens worldwide. A review of the literature reveals that many climatic variables, functioning singly or in combination, exert varying effects on the distribution and range of Culicoides vector midges and mosquitoes. For example, higher temperatures may be associated with increased insect abundancethereby amplifying the risk of disease transmission -but there are no indications yet of dramatic shifts occurring in the geographic range of Culicoides midges. However, the same cannot be said for mosquitoes: over the last few decades, multiple Asian species have established themselves in Europe, spread and are unlikely to ever be eradicated. Research on how insects respond to changes in climate is still in its infancy. The authors argue that we need to grasp how other annectant changes, such as extremes in precipitation (drought and flooding), may affect the dispersal capability of mosquitoes. Models are useful for assessing the interplay between mosquito vectors expanding their range and the native flora and fauna; however, ecological studies employing classical mark-release-recapture techniques remain essential for addressing fundamental questions about the survival and dispersal of mosquito species, with the resulting parameters fed directly into new-generation disease transmission models. Studies on the eventual impact of mosquitoes on animal and human health should be tackled through large-scale integrated research programmes. Such an approach calls for more collaborative efforts, along the lines of the One Health Initiative.

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“…Hence, the potential effect of fluorescent marking on the behavior during the off‐host state which axiomatically involves walking must be evaluated before application of this method. Previous studies have shown that fluorescent marking does not negatively affect multiple organisms . However, caution is warranted when applying this method to different organisms as chemical application may have distinct effects .…”

Section: Discussionmentioning

confidence: 99%

Assessment of potential effects and detection efficacy of a fluorescent marking system on a medically important hard tick,Haemaphysalis longicornis(Acari: Ixodidae)

Kim

Kho

Jung

et al. 2019

Pest Management Science

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BACKGROUND Although Haemaphysalis longicornis (Acari: Ixodidae) is an important disease vector, its small size restricts the tracking methods applicable. Recently, fluorescent marking as a conventional detection method for small arthropods has been improved by combining it with an ultraviolet laser. We examined the application potential of this new fluorescent marking system (FMS) for tracking H. longicornis by evaluating the effect of fluorescent marking on the ticks and detection efficacy. RESULTS Under laboratory conditions, fluorescent marking did not significantly affect the survivorship, movement patterns, and CO2 response of H. longicornis at all three developmental stages. Fluorescent‐marked individuals could be detected at distances ranging from 12 to 29 m under dark, increasing with the body size. Finally, in grassland, >90% of fluorescent‐marked individuals were retrieved at night regardless of developmental stage. However, the overall detection rate (<42%) was substantially reduced during the day. CONCLUSIOIN Our results show that FMS can reliably detect H. longicornis at night. Nevertheless, fluorescent‐marked individuals are not as conspicuous under sunlight when they are illuminated with ultraviolet lasers, limiting the use of FMS during the day. Therefore, the development of an alternative tracking method is warranted for an effective detection of ticks during the day. © 2019 Society of Chemical Industry

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Advances in methods for colour marking of mosquitoes

Cited by 60 publications

References 28 publications

Variation in host preferences of malaria mosquitoes is mediated by skin bacterial volatiles

Variation in host preferences of malaria mosquitoes is mediated by skin bacterial volatiles

Mosquitoes and Culicoides biting midges: vector range and the influence of climate change

Assessment of potential effects and detection efficacy of a fluorescent marking system on a medically important hard tick,Haemaphysalis longicornis(Acari: Ixodidae)

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