Advances in methodology for the validation of methods according to the International Organization for Standardization

Garcı́a, Inmaculada; Ortiz, M.C.; Sarabia, L.A.; Vilches, Carmen; Gredilla, Elisa

doi:10.1016/s0021-9673(03)00246-2

Cited by 67 publications

(13 citation statements)

References 13 publications

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“…Both calibrations were built with standards in concentrations within the linear range reported previously. The parameters of both regressions were determined following the method described in the next paragraph and the sensitivities were compared using the t-test as described by García et al [21]. The statistics calculated were smaller than the tabulated ones for the significance level set to 0.05 but bigger than the ones tabulated for a significance of 0.1.…”

Section: Analytical Performancementioning

confidence: 99%

Determination of bile acids in human serum by on-line restricted access material–ultra high-performance liquid chromatography–mass spectrometry

Bentayeb

Batlle

Sánchez

et al. 2008

Journal of Chromatography B

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Section: Analytical Performancementioning

confidence: 99%

Determination of bile acids in human serum by on-line restricted access material–ultra high-performance liquid chromatography–mass spectrometry

Bentayeb

Batlle

Sánchez

et al. 2008

Journal of Chromatography B

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“…For example, in soft drinks, European Union legislation establishes the limit of 150 mg/kg, Australia, 400 mg/kg, and Korea, 600 mg/kg, respectively. 3 The acceptable daily intake (ADI) for benzoic acid is 5 mg/kg body weight.…”

Section: -3mentioning

confidence: 99%

Analysis of Benzoic Acid in Quasi-Drug Drink Using Isotope Dilution Liquid Chromatography Mass Spectrometry

2008

Bulletin of the Korean Chemical Society

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An analytical method was developed for the accurate determination of benzoic acid in commercial quasi-drug drinks using isotope dilution liquid chromatography mass spectrometry (ID-LC/MS). Sample drinks were spiked with 13 C 6 -benzoic acid and these mixtures were cleaned by a nylon membrane filter. The sample mixtures were then analyzed using LC/Tandem MS in the negative ion mode without further clean-up or extraction steps. For the chromatographic separation, a C18 column with isocratic mobile phase of 50:50 (v/v) ammonium acetate buffer at pH 4.5 and methanol mixture was used. MS was operated with electrospray ionization in the negative mode. Benzoic acid and its isotope analogue were detected by selectively monitoring the collisionally-induced dissociation channels of− at m/z 121 → 77 and m/z 127 → 83, respectively. Repeatability and reproducibility studies showed that the ID-LC/MS/MS method is a reliable and reproducible method which can provide less than 0.3% of relative standard deviation (RSTD) of method precision for analysis of benzoic acid.

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“…Sodium benzoate (E211) is sodium salt of benzoic acid, and it is used in a variety of products, such as food, cosmetics, and pharmaceuticals, but more commonly in beverages to preserve freshness [4,5]. The undissociated form, formed from the salt dissolved in solution, is responsible for the antibacterial activity, which is optimum at a pH value between 2.5 and 4.0 (benzoic acid pK a 4.19).…”

Section: Introductionmentioning

confidence: 99%

Development of reverse-phase high-performance liquid chromatography method for simultaneous determination of sodium benzoate and potassium sorbate in beverages

Petanovska‐Ilievska

Velkoska-Markovska

Jankulovska

2017

Acta Chromatographica

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Summary.Reverse-phase high-performance liquid chromatography (RP-HPLC) method for simultaneous determination of sodium benzoate and potassium sorbate in beverages was developed using high speed column. The simple and rapid reverse-phase method for quantitative determination of both preservatives was established on LiChroCART ® Purospher STAR RP-18e (30 mm × 4 mm; 3 μm) column, mobile phase consisted of acetonitrile-phosphate buffer (pH = 3.5) in volume ratio of 8:92 (v/v), flow rate of 1 mL min −1 , ultraviolet (UV) detection at 195 nm for sodium benzoate and 260 nm for potassium sorbate, and constant column temperature at 25 °C. Linearity, precision, accuracy, limit of quantification (LOQ), and limit of detection (LOD) were tested for method validation. Linearity range for sodium benzoate was 6.04-200.27 mg L −1 (R 2 = 0.999) while, for potassium benzoate (R 2 = 0.999), 12.19-406.36 mg L −1 . The RSD values ≤1.03% demonstrate excellent intra-day precision. LOD for sodium benzoate and potassium sorbate was 0.004 and 0.003 mg L −1 , while LOQ was 0.012 and 0.009 mg L −1 , respectively. This method was applied for quantitative determination of investigated preservatives in beverages which were taken from Macedonian markets.

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Advances in methodology for the validation of methods according to the International Organization for Standardization

Cited by 67 publications

References 13 publications

Determination of bile acids in human serum by on-line restricted access material–ultra high-performance liquid chromatography–mass spectrometry

Determination of bile acids in human serum by on-line restricted access material–ultra high-performance liquid chromatography–mass spectrometry

Analysis of Benzoic Acid in Quasi-Drug Drink Using Isotope Dilution Liquid Chromatography Mass Spectrometry

Development of reverse-phase high-performance liquid chromatography method for simultaneous determination of sodium benzoate and potassium sorbate in beverages

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