Advances in metabolism and genetic control of astringency in persimmon (Diospyros kaki Thunb.) fruit: A review

Amorim, Catherine; Antoniolli, L. R.; Orsi, Bruna; Kluge, Ricardo Alfredo

doi:10.1016/j.scienta.2022.111561

Cited by 4 publications

(7 citation statements)

References 77 publications

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“…This aligns with the results of sensory evaluation. Xanthosine mainly affects the color, aroma, and astringency of dried French plum fruits [38]. Compared with untreated dried French plums, various osmotic sugar treatments decreased the xanthosine content, which suggests that such treatments can reduce the astringency of French plum products.…”

Section: Analysis Of Differential Metabolites By Volcanic Maps and Ve...mentioning

confidence: 95%

Objective Quantification Technique and Widely Targeted Metabolomics-Based Analysis of the Effects of Different Saccharidation Processes on Preserved French Plums

Yan,

Dong,

Yang

et al. 2024

Molecules

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Vacuum saccharification significantly affected the flavor and color of preserved French plums. However, the correlation between color, flavor, and metabolites remains unclear. Metabolites contribute significantly to enhancing the taste and overall quality of preserved French plums. This study aimed to investigate the distinctive metabolites in samples from various stages of the processing of preserved French plums. The PCF4 exhibited the highest appearance, overall taste, and chroma. Furthermore, utilizing UPLC and ESI-Q TRAP-MS/MS, a comprehensive examination of the metabolome in the processing of preserved French plums was conducted. A total of 1776 metabolites were analyzed. Using WGCNA, we explored metabolites associated with sensory features through 10 modules. Based on this, building the correlation of modules and objective quantification metrics yielded three key modules. After screening for 151 differentiated metabolites, amino acids, and their derivatives, phenolic acids, flavonoids, organic acids, and other groups were identified as key differentiators. The response of differential metabolites to stress influenced the taste and color properties of preserved prunes. Based on these analyses, six important metabolic pathways were identified. This study identified changes in the sensory properties of sugar-stained preserved prunes and their association with metabolite composition, providing a scientific basis for future work to improve the quality of prune processing.

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Section: Analysis Of Differential Metabolites By Volcanic Maps and Ve...mentioning

confidence: 95%

Objective Quantification Technique and Widely Targeted Metabolomics-Based Analysis of the Effects of Different Saccharidation Processes on Preserved French Plums

Yan,

Dong,

Yang

et al. 2024

Molecules

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“…To reveal the key factors involved in the natural deastringency of C-PCNA, homologybased cloning, high-throughput sequencing, and proteomic analysis were utilized, and many transcription factors and miRNAs were identified. A gene homologous to DkMYB4 was isolated from C-PCNA, named DkPA1, which regulates DkLAR in both C-PCNA and non-PCNA and plays a more important role in C-PCNA than in J-PCNA [2]. Moreover, DkMYC1 was cloned and characterized in C-PCNA, which may be an important bHLH (basic helix-loop-helix) gene involved in PAs biosynthesis in persimmon [2].…”

Section: Regulation Network Is Involved In the Natural Deastringency ...mentioning

confidence: 99%

“…A gene homologous to DkMYB4 was isolated from C-PCNA, named DkPA1, which regulates DkLAR in both C-PCNA and non-PCNA and plays a more important role in C-PCNA than in J-PCNA [2]. Moreover, DkMYC1 was cloned and characterized in C-PCNA, which may be an important bHLH (basic helix-loop-helix) gene involved in PAs biosynthesis in persimmon [2]. Through the combined analysis of transcriptome sequencing and proteome sequencing in C-PCNA fruit at 10 and 20 WAB, as well as the samples (10 WAB) being treated with warm water for 12 h or at room temperature for 12 h, two MYB genes were screened, named DkMYB14 and DkMYB15.…”

Section: Regulation Network Is Involved In the Natural Deastringency ...mentioning

confidence: 99%

“…The bioinformatics analysis of the transcriptome data of C-PCNA, J-PCNA, and non-PCNA at 2.5, 10, 20, and 25 WAB identified the DkMYB21 gene, which interacts with the bHLH-type transcription factor DkMYC1 and activates the expression of the structural genes DkPAL, DkF3'5'H, DkDFR, DkANS, and DkANR and the transcription factor DkMYB4 to regulate the synthesis of PAs [48]. In addition, two WRKY transcription factors, DkWRKY3/15, could be upstream of DkPK1 and positively regulate the natural deastringency of C-PCNA persimmon [2]. After the highthroughput sequencing of small RNA in C-PCNA fruit at 15 and 20 WAB, many miRNAs involved in natural deastringency were screened [49], among which miR858b was verified to repress DkMYB19 and DkMYB20 and decrease the biosynthesis of soluble tannins, and DkMYB20 contributes to the regulation of DkANR to control PAs biosynthesis [2].…”

Section: Regulation Network Is Involved In the Natural Deastringency ...mentioning

confidence: 99%

“…The C-PCNA trait of astringency loss is dominant (CPCNA) over non-PCNA types, while the J-PCNA is recessive (AST/ast), controlled by a different allele. In addition, the non-PCNA type includes the pollination-variant non-astringent (PVNA), pollination-variant astringent (PVA), and pollination-constant astringent (PCA) types [2][3][4][5]. Therefore, only J-PCNA hybridization could obtain a PCNA in the F1 generation; if J-PCNA was crossed with non-PCNA, there would be no PCNA individual in the F1 generation, and even if it was backcrossed with PCNA, the proportion of PCNA in the F2 generation would be only 15~20%.…”

Section: Introductionmentioning

confidence: 99%

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Recent Advances in Natural Deastringency and Genetic Improvement of Chinese PCNA Persimmon (Diospyros kaki)

Yang,

Zhang,

et al. 2023

Horticulturae

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Persimmon (Diospyros kaki) is a worldwide fruit cultivated mainly in the East Asia, Mediterranean, Caucasus, Latin America, and Oceania regions. This fruit contains abundant proanthocyanidins (PAs, also called condensed tannins), whose biosynthesis is the main cause of fruit astringency. As the original centre and top producing country, China has discovered a unique type with desirable natural deastringency, the Chinese pollination-constant non-astringent (C-PCNA) persimmon. Studies have revealed that the C-PCNA trait is controlled by a single and dominant locus, which differs from that of another type, the Japanese PCNA type, with recessive loci. In the C-PCNA type, accumulating evidence has shown that the astringency removal process involves two pathways (“dilution effect” and “coagulation effect”). Moreover, molecular marker-assisted selection (MAS) for the natural deastringency trait locus in C-PCNA has been used to test the non-astringency/astringency trait of hybrid offspring at the seedling stage. Importantly, persimmon can bear male flowers, female flowers, and perfect flowers, but sex-linked MAS has been developed for female-only persimmon. This sex-linked MAS, together with astringency-linked MAS and embryo rescue technology, may even shorten the conventional cross-breeding period of about 2–3 years. In addition, recently studies have established a stable genetic transformation system for persimmon transgenic improvement. Despite these efforts, how synthetic PAs and metabolism pathways lead to a deastringent trait remains unclear for persimmon. Thus, our review summarizes the latest research progress on the natural deastringency mechanism in C-PCNA, and we provide a new viewpoint for the genetic improvement of persimmon breeding in China.

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DkDTX1/MATE1 mediates the accumulation of proanthocyanidin and affects astringency in persimmon

Liu,

Sun,

et al. 2024

Plant Cell & Environment

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Proanthocyanidins (PAs) is a kind of polyphenols widely distributed in plants, and their astringent properties can protect plants from herbivores and regulate fruit taste. There is a great difference in PA composition between astringent (A)‐type and nonastringent (NA)‐type persimmon. Here, we studied the potential of DkDTX1/MATE1 in regulating PAs composition through its preferred transport in persimmon fruit. The results of fluorescence microscope showed that the DkDTX1/MATE1 green fluorescence overlapped with the blue light emitted by PA. Overexpression of DkDTX1/MATE1 in persimmon leaves not only significantly increase the concentrations of PA, but also upregulated the expression of PA biosynthesis pathway genes. Further overexpression of DkDTX1/MATE1 in persimmon fruit discs and stable genetic transformation of DkDTX1/MATE1 also led to PA concentrations increased. Molecular docking and transporter assays showed that DkDTX1/MATE1 preferentially transported catechin, epicatechin gallate and epigallocatechin gallate. DkDTX1/MATE1 mainly bound to the PA precursors via serine at position 68. Our findings indicate that DkDTX1/MATE1 play a role in the accumulation of PAs in early stage of fruit development and affects the astringency of persimmon through preferential transport PA precursors, which provided a theoretical basis for the future use of metabolic engineering to regulate the composition of PAs in persimmon.

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Advances in metabolism and genetic control of astringency in persimmon (Diospyros kaki Thunb.) fruit: A review

Cited by 4 publications

References 77 publications

Objective Quantification Technique and Widely Targeted Metabolomics-Based Analysis of the Effects of Different Saccharidation Processes on Preserved French Plums

Objective Quantification Technique and Widely Targeted Metabolomics-Based Analysis of the Effects of Different Saccharidation Processes on Preserved French Plums

Recent Advances in Natural Deastringency and Genetic Improvement of Chinese PCNA Persimmon (Diospyros kaki)

DkDTX1/MATE1 mediates the accumulation of proanthocyanidin and affects astringency in persimmon

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