Advances in mass spectrometry-based metabolomics for investigation of metabolites

Ren, Junling; Zhang, Aihua; Kong, Ling; Wang, Xijun

doi:10.1039/c8ra01574k

Cited by 206 publications

(146 citation statements)

References 172 publications

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“…Metabolomics approach plays a key role in researching the metabolic disorder-related diseases (Zhang et al, 2013d;Zhang et al, 2014e;Zhang et al, 2015b;Sun et al, 2015;Zhao et al, 2016;Chu et al, 2016;Song et al, 2017;Ren et al, 2018;Qiu et al, 2020), discovery and identification of biomarkers (Zhang et al, 2014f) and treatment (Wang et al, 2012;Liang et al, 2015;Zhang et al, 2016b;Zhao et al, 2017;Liu et al, 2018;Sun et al, 2019;Wu et al, 2019;Qiu et al, 2020). Many biomarkers are being discovered for better understanding of metabolic mechanisms (Ma et al, 2016; and evaluating the efficacy diseases (Chu et al, 2015a;Chu et al, 2015b;Zhang et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

High-Throughput Metabolomics for Identification of Metabolic Pathways and Deciphering the Effect Mechanism of Dioscin on Rectal Cancer From Cell Metabolic Profiles Coupled With Chemometrics Analysis

Wang

2020

Front. Pharmacol.

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High-throughput liquid chromatography-mass spectrometry (LC-MS)-based metabolomics can provide the holistic analysis of the low molecular weight endogenous metabolites in cells and reflect the changes of cellular regulation and metabolic pathways. Our study designed to reveal the potentially pharmacological effects of dioscin on SW480 rectal cancer cells using nontargeted metabolomics method to probe into small molecular metabolites and pathway changes. After the cell assay of proliferation, apoptosis, migration, and invasion, the dioscin-treated cell samples were prepared for nontargeted metabolomics analysis based on LC-MS tool to describe the metabolic profiles. Dioscin has prevented cell proliferation and accelerated cell apoptosis, and it also inhibited the SW480 rectal cancer cells' migration and invasion. A total of 22 metabolites were selected as promising biomarkers of pharmacological reaction of dioscin to rectal cancer, and eight highly correlated pathways including D-glutamine and D-glutamate metabolism, pyruvate metabolism, arachidonic acid metabolism, phenylalanine metabolism, tryptophan metabolism, glycolysis or gluconeogenesis, citrate cycle (TCA cycle), and butanoate metabolism were identified. It showed that strategies based on cell metabolomics are helpful tools to discover the small molecular metabolites to elucidate the action mechanism of drug.

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Section: Discussionmentioning

confidence: 99%

High-Throughput Metabolomics for Identification of Metabolic Pathways and Deciphering the Effect Mechanism of Dioscin on Rectal Cancer From Cell Metabolic Profiles Coupled With Chemometrics Analysis

Wang

2020

Front. Pharmacol.

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“…In addition, CE has poor migration time reproducibility and a lack of reference libraries, which may only be partially overcome by the prediction of migration time [103,104]. However, CE has some distinct advantages over other instruments employed for metabolomics: mainly the facts that it utilizes low separation volume, which is particularly suitable for the study of biological fluids in small experimental animals, and operates under a homogeneous separation environment [105].…”

Section: Capillary Electrophoresis-mass Spectrometry (Ce-ms)mentioning

confidence: 99%

Metabolomics in the Context of Plant Natural Products Research: From Sample Preparation to Metabolite Analysis

et al. 2020

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Plant-derived natural products have long been considered a valuable source of lead compounds for drug development. Natural extracts are usually composed of hundreds to thousands of metabolites, whereby the bioactivity of natural extracts can be represented by synergism between several metabolites. However, isolating every single compound from a natural extract is not always possible due to the complex chemistry and presence of most secondary metabolites at very low levels. Metabolomics has emerged in recent years as an indispensable tool for the analysis of thousands of metabolites from crude natural extracts, leading to a paradigm shift in natural products drug research. Analytical methods such as mass spectrometry (MS) and nuclear magnetic resonance (NMR) are used to comprehensively annotate the constituents of plant natural products for screening, drug discovery as well as for quality control purposes such as those required for phytomedicine. In this review, the current advancements in plant sample preparation, sample measurements, and data analysis are presented alongside a few case studies of the successful applications of these processes in plant natural product drug discovery.

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“…MS techniques that do not require a separation step e.g. MALDI (matrix-assisted laser desorption ionization) MS are widely used in salivary proteomics and while such approaches offer promise in metabolomics of other substances, this is not yet common in saliva [27]. Several reviews have been written about the relative advantages and limitations of different MS and NMR platforms in metabolomics [28][29][30][31][32][33].…”

Section: Approaches To the Study Of Salivary Metabolitesmentioning

confidence: 99%

Salivary Metabolomics: From Diagnostic Biomarker Discovery to Investigating Biological Function

2020

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Metabolomic profiling of biofluids, e.g., urine, plasma, has generated vast and ever-increasing amounts of knowledge over the last few decades. Paradoxically, metabolomic analysis of saliva, the most readily-available human biofluid, has lagged. This review explores the history of saliva-based metabolomics and summarizes current knowledge of salivary metabolomics. Current applications of salivary metabolomics have largely focused on diagnostic biomarker discovery and the diagnostic value of the current literature base is explored. There is also a small, albeit promising, literature base concerning the use of salivary metabolomics in monitoring athletic performance. Functional roles of salivary metabolites remain largely unexplored. Areas of emerging knowledge include the role of oral host–microbiome interactions in shaping the salivary metabolite profile and the potential roles of salivary metabolites in oral physiology, e.g., in taste perception. Discussion of future research directions describes the need to begin acquiring a greater knowledge of the function of salivary metabolites, a current research direction in the field of the gut metabolome. The role of saliva as an easily obtainable, information-rich fluid that could complement other gastrointestinal fluids in the exploration of the gut metabolome is emphasized.

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Advances in mass spectrometry-based metabolomics for investigation of metabolites

Cited by 206 publications

References 172 publications

High-Throughput Metabolomics for Identification of Metabolic Pathways and Deciphering the Effect Mechanism of Dioscin on Rectal Cancer From Cell Metabolic Profiles Coupled With Chemometrics Analysis

High-Throughput Metabolomics for Identification of Metabolic Pathways and Deciphering the Effect Mechanism of Dioscin on Rectal Cancer From Cell Metabolic Profiles Coupled With Chemometrics Analysis

Metabolomics in the Context of Plant Natural Products Research: From Sample Preparation to Metabolite Analysis

Salivary Metabolomics: From Diagnostic Biomarker Discovery to Investigating Biological Function

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