Advances in inner ear gene therapy: exploring cochlear protection and regeneration

Maiorana, Carrie R; Staecker, Hinrich

doi:10.1097/01.moo.0000179248.51476.11

Cited by 11 publications

(9 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Viral vectors have superior gene delivery efficacy but safety concern have arisen. Thus the clinical application of gene therapy is somewhat limited by the potentially adverse effects of the virus itself, such as immunogenicity, toxicity and possible mutagenesis of the transfected cells [89,136]. Thus, using non-viral vector technology would be a safer alternative.…”

Section: Transfer Vectors For Cochlear Gene Therapymentioning

confidence: 99%

“…Liposomes have very low risk of causing an immune response and insertional mutagenesis. But the disadvantages are inefficient gene transfer and low rate of transfection [88,135,136]. Nanoparticles have been investigated as an alternative to viral vector systems, but carry concerns about safety as well as precision of targeting of specific cells and tissues [148].…”

Section: Transfer Vectors For Cochlear Gene Therapymentioning

confidence: 99%

See 1 more Smart Citation

Use of the biodegradable polymer chitosan as a vehicle for applying drugs to the inner ear

Saber

Strand

Ulfendahl

2010

European Journal of Pharmaceutical Sciences

View full text Add to dashboard Cite

Section: Transfer Vectors For Cochlear Gene Therapymentioning

confidence: 99%

Section: Transfer Vectors For Cochlear Gene Therapymentioning

confidence: 99%

Use of the biodegradable polymer chitosan as a vehicle for applying drugs to the inner ear

Saber

Strand

Ulfendahl

2010

European Journal of Pharmaceutical Sciences

View full text Add to dashboard Cite

“…Currently, inner-ear gene therapy is undergoing a transition from simple feasibility experiments to exploration of the safe delivery of potentially therapeutic molecules in animal models of inner-ear dysfunction [1]. A key step, therefore, is to select a suitable vector for transfecting the cells, and to have this vector targeted to specific cells in the cochlea.…”

Section: Introductionmentioning

confidence: 99%

Efficient transduction of spiral ganglion neurons in vitro by baculovirus vectors

Wang¹,

Cai³

et al. 2007

NeuroReport

View full text Add to dashboard Cite

Degeneration of peripheral auditory neurons constitutes one of the main causes of sensorineural hearing loss. Gene delivery to the inner ear is central to the development of gene therapy for hearing impairment. Thus we investigated the effectiveness of baculovirus-derived vectors to transduce spiral ganglion neurons. We found that baculovirus could efficiently transduce spiral ganglion neurons in vitro and that the highest transduced cell rate could be over 75%. The level of transgene expression exhibited viral dose dependence and was enhanced by the addition of butyrate. Thus, baculovirus is a novel and promising tool for gene transfer into the cochlear nervous system, both in studies of the function of foreign genes and in the development of gene-therapy strategies.

show abstract

“…20,24 Most studies are currently being performed with adenoviruses, although there are still ongoing experiments with AAV and Herpes simplex virus (HSV) vectors because of their potential ability to achieve long-term expression. 25 REGENERATION IN THE COCHLEA The source of new hair cells in regenerating inner ear sensory epithelia of lower vertebrates, as well as in the mammalian vestibular organ, is the supporting cells. [26][27][28][29][30] This led to the assumption that manipulation of supporting cells in the organ of Corti could induce hair cell regeneration.…”

Section: Vectors For Inner Ear Deliverymentioning

confidence: 99%

Future Trends and Potential for Treatment of Sensorineural Hearing Loss

Brownstein¹,

Avraham²

2006

Semin Hear

View full text Add to dashboard Cite

Damage and loss of hair cells in the inner ear is the most frequent cause of hearing loss (HL), since mammalian hair cells are not replenished once lost. To date, the treatment of HL consists of hearing aids or cochlear implants, but both options restore hearing with limited success. Gene therapy is an attractive option as a means for treating sensorineural hearing impairment, although at present it is investigational. The finding that hair cells can regenerate in nonmammalian vertebrates has triggered many studies, with the hope of simulating a similar process in humans. Current research that focuses on the auditory pathways, the genes involved in HL, efficient ways to transfer therapeutic agents into the inner ear, and optimization of gene manipulation, gene therapy, and stem cell treatment raises hope for restoring hearing and curing deafness.Learning Outcomes: As a result of this activity, the participant will be able to (1) list the different approaches being studied for the treatment of sensorineural hearing loss, and (2) list the methods used to transfer therapeutic genes into the inner ear.Hearing impairment affects approximately one in 1000 newborns and 4% of people under the age of 45. 1 Progressive hearing loss (HL) affects a greater proportion of the population; in the United States 10% of the population suffers from HL by age 65 and 50% by age 80. 2 Similar proportions exist in other parts of the world, except where high rates of consanguinity are endemic, such as the Middle East, India, and Pakistan. 3 In these regions, large kindreds have been identified with a higher rate of deafness, particularly in children.Various genetic and environmental causes are involved in the etiology of sensorineural

show abstract

Advances in inner ear gene therapy: exploring cochlear protection and regeneration

Cited by 11 publications

References 44 publications

Use of the biodegradable polymer chitosan as a vehicle for applying drugs to the inner ear

Use of the biodegradable polymer chitosan as a vehicle for applying drugs to the inner ear

Efficient transduction of spiral ganglion neurons in vitro by baculovirus vectors

Future Trends and Potential for Treatment of Sensorineural Hearing Loss

Contact Info

Product

Resources

About