Advances in hemoglobinopathy detection and identification

Matrix‐assisted laser desorption/ionization imaging mass spectrometry of intraperitoneally injected danegaptide (ZP1609) for treatment of stroke‐reperfusion injury in mice

“…Although α‐cyano‐4‐hydroxycinnamic acid (CHCA) has been shown to be effective for low‐mass peptide analysis, it generates an abundance of matrix background signals of high signal intensity within the m/z 100–500 range and thus the CHCA peaks suppressed the ZP1609 signal. Although SA is normally used for the analysis of small proteins, both DHB and SA matrices have been found to be highly effective for MALDI IMS analysis; therefore, both matrices were tested for ZP1609 analysis . For matrix comparisons, brain tissue samples from the experimental group with IP‐injected ZP1609 were coated with either SA or DHB and profiling data were collected (Figure ).…”

“…Although SA is normally used for the analysis of small proteins, both DHB and SA matrices have been found to be highly effective for MALDI IMS analysis; therefore, both matrices were tested for ZP1609 analysis. 32,33 For matrix comparisons, brain tissue samples from the experimental group with IP-injected ZP1609 were coated with either SA or DHB and profiling data were collected ( Figure 2). Of these two matrices, DHB was found to provide the greater detection sensitivity of the target analytes with minimal interference from background.…”

Expanded Newborn Screening Program in Saudi Arabia: Incidence of screened disorders

“…MS/MS is cost‐effective to use in newborn screening (NBS) as one test will diagnose several disorders and it requires only few drops of blood from the newborn. MS/MS allows for rapid, simultaneous analysis and detection followed by second tier conformation of plenty of metabolic diseases as amino acid, organic acid and fatty acid defects in addition to haemoglobinopathies and endocrine disorders …”

Anemia infantil

“…Otras hemoglobinopatías incluyen la hemoglobina E, la hemoglobinopatía más común en todo el mundo. La anemia hemolítica también puede ser causada por infección, hemangiomas, deficiencia de vitamina E y coagulación intravascular diseminada, entre otros trastornos 23 .…”