Advances in Genetic Transformation

Visarada, K. B. R. S.; Kishore, N. Sai

doi:10.1007/978-81-322-2422-8_9

Cited by 7 publications

(4 citation statements)

References 55 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Immature embryos are the predominant explants used for the transformation of several crop species (Figure 2B) [61][62][63][64], and the efficiency of the system depends on various physiological and environmental factors [65].…”

Section: Immature Embryosmentioning

confidence: 99%

Opportunities and Challenges of In Vitro Tissue Culture Systems in the Era of Crop Genome Editing

Bekalu

Panting

Holme

et al. 2023

IJMS

View full text Add to dashboard Cite

Currently, the development of genome editing (GE) tools has provided a wide platform for targeted modification of plant genomes. However, the lack of versatile DNA delivery systems for a large variety of crop species has been the main bottleneck for improving crops with beneficial traits. Currently, the generation of plants with heritable mutations induced by GE tools mostly goes through tissue culture. Unfortunately, current tissue culture systems restrict successful results to only a limited number of plant species and genotypes. In order to release the full potential of the GE tools, procedures need to be species and genotype independent. This review provides an in-depth summary and insights into the various in vitro tissue culture systems used for GE in the economically important crops barley, wheat, rice, sorghum, soybean, maize, potatoes, cassava, and millet and uncovers new opportunities and challenges of already-established tissue culture platforms for GE in the crops.

show abstract

Section: Immature Embryosmentioning

confidence: 99%

Opportunities and Challenges of In Vitro Tissue Culture Systems in the Era of Crop Genome Editing

Bekalu

Panting

Holme

et al. 2023

IJMS

View full text Add to dashboard Cite

show abstract

“…However, understanding the biology of the early stages of Striga parasitism will help identify potential barriers to the success of this technology. Although significant strides have been made in genetic transformation, there have been very few reports of sorghum crops lagging behind other cereals such as maize in terms of genetic transformation (Visarada and Kishore, 2015) [81] . Regardless of the varying successes recorded by different workers in attempts to induce Striga resistance, all reports reflect a great potential of RNAi in the development of transgenic maize capable of suppressing the parasite's proliferation.…”

Section: Role Of Genetic Engineering In Improving Maize Resistance To...mentioning

confidence: 99%

Review on methods of maize breeding for tolerance or resistance of Striga hermonthica

Gichile

2022

Int. J. Res. Agron.

View full text Add to dashboard Cite

Striga hermonthica is a major limiting factor in maize cultivation in sub-Saharan Africa. Based on environmental factors such as soil fertility, climatic conditions and genotype, this can result in a yield loss of up to 100%. The aim of this article is to review maize breeding methods for resistance/tolerance to Striga hermontica and to highlight the efforts made to overcome yield reduction due to Striga hermonthica. The International Institute of Tropical Agriculture (IITA) has made significant progress in developing several early inbred maize lines, open-pollinated varieties and hybrids containing Striga resistance genes. However, the levels of striga resistance of the available precocious inbreds and hybrids are not as high as desired. This is because the Striga seed bank is added to the soil after each growing season. There is therefore a need for increased Striga resistance in the available genotypes. Since resistance to S. hermonthica in maize is regulated by many genes, breeding for Striga-resistant cultivars using traditional approaches has been less effective and time-consuming. The identification of QTLs associated with Striga resistance/tolerance would facilitate the rapid development of Striga resistant/tolerant maize genotypes.

show abstract

“…Despite several decades of study, sorghum has been widely considered as a recalcitrant major crop for transformation [ 5 ], due to tissue culture limitations (accumulation of phenolic compounds), lack of model genotypes, low regeneration frequency and loss of regeneration potential through sub-cultures [ 6 ]. As a result, a highly efficient and comprehensive transformation system has remained elusive till date [ 7 ].…”

Section: Introductionmentioning

confidence: 99%

“…Among different explants used for successful genetic transformation of sorghum (PDS or Agrobacterium ), immature embryos are the choice of explants over mature seed derived explants (shoot tip) and all the efficient transformation protocols by PDS or Agrobacterium largely employed immature embryos [ 12 , 15 , 16 ]. One of the main challenges of using immature embryos directly as explants is the need for continued planting of stock plants to ensure a constant supply of immature embryos which is difficult because restrictive conditions for flowering allows only a small window for collection of suitable embryos [ 6 ]. The cost and labour associated with immature embryo isolation is significant especially when large numbers are required to generate hundreds and occasionally thousands of transgenic events.…”

Section: Introductionmentioning

confidence: 99%

Robust genetic transformation of sorghum (Sorghum bicolor L.) using differentiating embryogenic callus induced from immature embryos

et al. 2017

View full text Add to dashboard Cite

BackgroundSorghum (Sorghum bicolor L.) is one of the world’s most important cereal crops grown for multiple applications and has been identified as a potential biofuel crop. Despite several decades of study, sorghum has been widely considered as a recalcitrant major crop for transformation due to accumulation of phenolic compounds, lack of model genotypes, low regeneration frequency and loss of regeneration potential through sub-cultures. Among different explants used for genetic transformation of sorghum, immature embryos are ideal over other explants. However, the continuous supply of quality immature embryos for transformation is labour intensive and expensive. In addition, transformation efficiencies are also influenced by environmental conditions (light and temperature). Despite these challenges, immature embryos remain the predominant choice because of their success rate and also due to non-availability of other dependable explants without compromising the transformation efficiency.ResultsWe report here a robust genetic transformation method for sorghum (Tx430) using differentiating embryogenic calli (DEC) with nodular structures induced from immature embryos and maintained for more than a year without losing regeneration potential on modified MS media. The addition of lipoic acid (LA) to callus induction media along with optimized growth regulators increased callus induction frequency from 61.3 ± 3.2 to 79 ± 6.5% from immature embryos (1.5–2.0 mm in length) isolated 12–15 days after pollination. Similarly, the regeneration efficiency and the number of shoots from DEC tissue was enhanced by LA. The optimized regeneration system in combination with particle bombardment resulted in an average transformation efficiency (TE) of 27.2 or 46.6% based on the selection strategy, 25% to twofold higher TE than published reports in Tx430. Up to 100% putative transgenic shoots were positive for npt-II by PCR and 48% of events had < 3 copies of transgenes as determined by digital droplet PCR. Reproducibility of this method was demonstrated by generating ~ 800 transgenic plants using 10 different gene constructs.ConclusionsThis protocol demonstrates significant improvements in both efficiency and ease of use over existing sorghum transformation methods using PDS, also enables quick hypothesis testing in the production of various high value products in sorghum.Electronic supplementary materialThe online version of this article (10.1186/s13007-017-0260-9) contains supplementary material, which is available to authorized users.

show abstract

Advances in Genetic Transformation

Cited by 7 publications

References 55 publications

Opportunities and Challenges of In Vitro Tissue Culture Systems in the Era of Crop Genome Editing

Opportunities and Challenges of In Vitro Tissue Culture Systems in the Era of Crop Genome Editing

Review on methods of maize breeding for tolerance or resistance of Striga hermonthica

Robust genetic transformation of sorghum (Sorghum bicolor L.) using differentiating embryogenic callus induced from immature embryos

Contact Info

Product

Resources

About