Advances in fluorescence microscopy can reveal important new aspects of tissue regeneration

Engelbrecht, Lize; Ollewagen, Tracey; Swardt, Dalene de

doi:10.1016/j.biochi.2022.02.001

Cited by 4 publications

(1 citation statement)

References 88 publications

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“…It has become critical in fluorescence microscopy, which offers many opportunities to label different structures to study the morphology and physiology of cells and tissues. [ 2–4 ] However, it also leads to photobleaching and phototoxicity when the specimen is illuminated with high‐intensity light. Photobleaching can underestimate the fluorescence intensity data recorded in a given sample, causing significant interference in time‐lapse imaging.…”

Section: Introductionmentioning

confidence: 99%

New approaches for low phototoxicity imaging of living cells and tissues

Kasprzycka,

Szumigraj,

Wachulak

et al. 2024

BioEssays

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Fluorescence microscopy is a powerful tool used in scientific and medical research, but it is inextricably linked to phototoxicity. Neglecting phototoxicity can lead to erroneous or inconclusive results. Recently, several reports have addressed this issue, but it is still underestimated by many researchers, even though it can lead to cell death. Phototoxicity can be reduced by appropriate microscopic techniques and carefully designed experiments. This review focuses on recent strategies to reduce phototoxicity in microscopic imaging of living cells and tissues. We describe digital image processing and new hardware solutions. We point out new modifications of microscopy methods and hope that this review will interest microscopy hardware engineers. Our aim is to underscore the challenges and potential solutions integral to the design of microscopy systems. Simultaneously, we intend to engage biologists, offering insight into the latest technological advancements in imaging that can enhance their understanding and practice.

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Section: Introductionmentioning

confidence: 99%

New approaches for low phototoxicity imaging of living cells and tissues

Kasprzycka,

Szumigraj,

Wachulak

et al. 2024

BioEssays

View full text Add to dashboard Cite

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Editorial: Regeneration in Health and Disease

Niesler

Vyver

2022

Biochimie

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Molecular and cellular imaging of the eye

Nguyen,

Zhe,

et al. 2023

Biomed. Opt. Express

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The application of molecular and cellular imaging in ophthalmology has numerous benefits. It can enable the early detection and diagnosis of ocular diseases, facilitating timely intervention and improved patient outcomes. Molecular imaging techniques can help identify disease biomarkers, monitor disease progression, and evaluate treatment responses. Furthermore, these techniques allow researchers to gain insights into the pathogenesis of ocular diseases and develop novel therapeutic strategies. Molecular and cellular imaging can also allow basic research to elucidate the normal physiological processes occurring within the eye, such as cell signaling, tissue remodeling, and immune responses. By providing detailed visualization at the molecular and cellular level, these imaging techniques contribute to a comprehensive understanding of ocular biology. Current clinically available imaging often relies on confocal microscopy, multi-photon microscopy, PET (positron emission tomography) or SPECT (single-photon emission computed tomography) techniques, optical coherence tomography (OCT), and fluorescence imaging. Preclinical research focuses on the identification of novel molecular targets for various diseases. The aim is to discover specific biomarkers or molecular pathways associated with diseases, allowing for targeted imaging and precise disease characterization. In parallel, efforts are being made to develop sophisticated and multifunctional contrast agents that can selectively bind to these identified molecular targets. These contrast agents can enhance the imaging signal and improve the sensitivity and specificity of molecular imaging by carrying various imaging labels, including radionuclides for PET or SPECT, fluorescent dyes for optical imaging, or nanoparticles for multimodal imaging. Furthermore, advancements in technology and instrumentation are being pursued to enable multimodality molecular imaging. Integrating different imaging modalities, such as PET/MRI (magnetic resonance imaging) or PET/CT (computed tomography), allows for the complementary strengths of each modality to be combined, providing comprehensive molecular and anatomical information in a single examination. Recently, photoacoustic microscopy (PAM) has been explored as a novel imaging technology for visualization of different retinal diseases. PAM is a non-invasive, non-ionizing radiation, and hybrid imaging modality that combines the optical excitation of contrast agents with ultrasound detection. It offers a unique approach to imaging by providing both anatomical and functional information. Its ability to utilize molecularly targeted contrast agents holds great promise for molecular imaging applications in ophthalmology. In this review, we will summarize the application of multimodality molecular imaging for tracking chorioretinal angiogenesis along with the migration of stem cells after subretinal transplantation in vivo.

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Advances in fluorescence microscopy can reveal important new aspects of tissue regeneration

Cited by 4 publications

References 88 publications

New approaches for low phototoxicity imaging of living cells and tissues

New approaches for low phototoxicity imaging of living cells and tissues

Editorial: Regeneration in Health and Disease

Molecular and cellular imaging of the eye

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