Advances in Escherichia coli Nissle 1917 as a customizable drug delivery system for disease treatment and diagnosis strategies

Chen, Haojie; Lei, Pengyu; Ji, Hao; Yang, Qinsi; Peng, Bo; Ma, Jiahui; Fang, Yimeng; Qu, Linkai; Li, Hua; Wu, Wei; Jin, Libo; Sun, Da

doi:10.1016/j.mtbio.2023.100543

Cited by 20 publications

(15 citation statements)

References 104 publications

(130 reference statements)

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“…For a proof-of-concept study, Escherichia coli Nissel 1917 (EcN), a probiotic well-known for its good antibacterial, antiinflammatory, and microbial homeostasis regulatory characteristics and widely used for treating a series of gastrointestinal diseases, [21] was selected as a model bacterial strain. A nanocoating strategy was designed to rapidly modify the surface of EcN.…”

Section: Construction and Characterization Of Coated Bacteriamentioning

confidence: 99%

Responsively Degradable Nanoarmor‐Assisted Super Resistance and Stable Colonization of Probiotics for Enhanced Inflammation‐Targeted Delivery

Zhu,

Yu,

Wang

et al. 2024

Advanced Materials

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Manipulation of the gut microbiota using oral microecological preparations has shown great promise in treating various inflammatory disorders. However, delivering these preparations while maintaining their disease‐site specificity, stability, and therapeutic efficacy is highly challenging due to the dynamic changes associated with pathological microenvironments in the gastrointestinal tract. Herein, a superior armored probiotic with an inflammation‐targeting capacity is developed to enhance the efficacy and timely action of bacterial therapy against inflammatory bowel disease (IBD). The coating strategy exhibits suitability for diverse probiotic strains and has negligible influence on bacterial viability. This study demonstrates that these armored probiotics have ultraresistance to extreme intraluminal conditions and stable mucoadhesive capacity. Notably, the HA‐functionalized nanoarmor equips the probiotics with inflamed‐site targetability through multiple interactions, thus enhancing their efficacy in IBD therapy. Moreover, timely “awakening” of ingested probiotics through the responsive transferrin‐directed degradation of the nanoarmor at the site of inflammation is highly beneficial for bacterial therapy, which requires the bacterial cells to be fully functional. Given its easy preparation and favorable biocompatibility, the developed single‐cell coating approach provides an effective strategy for the advanced delivery of probiotics for biomedical applications at the cellular level.This article is protected by copyright. All rights reserved

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Section: Construction and Characterization Of Coated Bacteriamentioning

confidence: 99%

Responsively Degradable Nanoarmor‐Assisted Super Resistance and Stable Colonization of Probiotics for Enhanced Inflammation‐Targeted Delivery

Zhu,

Yu,

Wang

et al. 2024

Advanced Materials

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“…Extensive studies have demonstrated that various types of bacteria (e.g., attenuated Salmonella, Escherichia coli, Clostridium butyricum, and photosynthetic bacteria) could be chemically decorated for solid tumor treatment. [24][25][26][27][28][29][30] The commonly used chemical modification is attaching nanoparticles onto bacterial surfaces via electrostatic interactions. [28,[31][32] However, this noncovalent modification usually leads to premature release of payloads.…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, these bacteria are considered to be promising candidates for tumor‐targeting drug delivery. Extensive studies have demonstrated that various types of bacteria (e.g., attenuated Salmonella , Escherichia coli , Clostridium butyricum , and photosynthetic bacteria) could be chemically decorated for solid tumor treatment [24–30] . The commonly used chemical modification is attaching nanoparticles onto bacterial surfaces via electrostatic interactions [28,31–32] .…”

Section: Introductionmentioning

confidence: 99%

Engineered Bacteria Labeled with Iridium(III) Photosensitizers for Enhanced Photodynamic Immunotherapy of Solid Tumors

Lin,

Liu,

Wang

et al. 2023

Angew Chem Int Ed

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Despite metal‐based photosensitizers showing great potential in photodynamic therapy for tumor treatment, the application of the photosensitizers is intrinsically limited by their poor cancer‐targeting properties. Herein, we reported a metal‐based photosensitizer‐bacteria hybrid, Ir‐HEcN, via covalent labeling of an iridium(III) photosensitizer to the surface of genetically engineered bacteria. Due to its intrinsic self‐propelled motility and hypoxia tropism, Ir‐HEcN selectively targets and penetrates deeply into tumor tissues. Importantly, Ir‐HEcN is capable of inducing pyroptosis and immunogenic cell death of tumor cells under irradiation, thereby remarkably evoking anti‐tumor innate and adaptive immune responses in vivo and leading to the regression of solid tumors via combinational photodynamic therapy and immunotherapy. To the best of our knowledge, Ir‐HEcN is the first metal complex decorated bacteria for enhanced photodynamic immunotherapy.

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“…2,28,29 Because of the importance of the topic and the advantages of using GEB such as broad tumor-targeting potentials, local therapeutic delivery, flexibility of delivery, enhanced host adaptability, facilitating the host's immune responses, reduced off-target toxicity, and improved in situ cancer therapy. 17,20,30,31 In this review, recent advancements, important challenges, and future prospects of GEB in targeted drug delivery and cancer therapy are deliberated.…”

Section: Introductionmentioning

confidence: 99%

Genetically engineered bacteria: a new frontier in targeted drug delivery

Fooladi,

Rabiee,

Iravani

2023

J. Mater. Chem. B

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Advances in Escherichia coli Nissle 1917 as a customizable drug delivery system for disease treatment and diagnosis strategies

Cited by 20 publications

References 104 publications

Responsively Degradable Nanoarmor‐Assisted Super Resistance and Stable Colonization of Probiotics for Enhanced Inflammation‐Targeted Delivery

Responsively Degradable Nanoarmor‐Assisted Super Resistance and Stable Colonization of Probiotics for Enhanced Inflammation‐Targeted Delivery

Engineered Bacteria Labeled with Iridium(III) Photosensitizers for Enhanced Photodynamic Immunotherapy of Solid Tumors

Genetically engineered bacteria: a new frontier in targeted drug delivery

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