Advances in endophytic fungi research: a data analysis of 25 years of achievements and challenges

Liu-Xu, Luisa; Vicedo, Begonya; García‐Agustín, Pilar; Llorens, Eugenio

doi:10.1080/17429145.2022.2032429

Cited by 17 publications

(13 citation statements)

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“…With research progress regarding endophytic microorganisms, fungal endophytes have historically held several definitions. Currently, the most accepted definition for established endophytic fungi is fungi that entirely reside within plant tissues without causing apparent disease symptoms ([ 47 ] and the references therein). Over the past two decades, there has been exponential growth in the research on this topic in terrestrial plants [ 47 ], whereas limited attention has been paid to fungal plant endophytes in marine habitats.…”

Section: Discussionmentioning

confidence: 99%

“…Currently, the most accepted definition for established endophytic fungi is fungi that entirely reside within plant tissues without causing apparent disease symptoms ([ 47 ] and the references therein). Over the past two decades, there has been exponential growth in the research on this topic in terrestrial plants [ 47 ], whereas limited attention has been paid to fungal plant endophytes in marine habitats. Hitherto, the vast majority of current insights into the endophytic fungi of marine algae were derived from macroalgae (see below).…”

Section: Discussionmentioning

confidence: 99%

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Different Geographic Strains of Dinoflagellate Karlodinium veneficum Host Highly Diverse Fungal Community and Potentially Serve as Possible Niche for Colonization of Fungal Endophytes

Deng

Wang

et al. 2023

IJMS

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In numerous studies, researchers have explored the interactions between fungi and their hosting biota in terrestrial systems, while much less attention has been paid to the counterpart interactions in aquatic, and particularly marine, ecosystems. Despite the growing recognition of the potential functions of fungi in structuring phytoplankton communities, the current insights were mostly derived from phytoplankton hosts, such as diatoms, green microalgae, and cyanobacteria. Dinoflagellates are the second most abundant group of phytoplankton in coastal marine ecosystems, and they are notorious for causing harmful algal blooms (HABs). In this study, we used high-throughput amplicon sequencing to capture global snapshots of specific fungal assemblages associated with laboratory-cultured marine dinoflagellate. We investigated a total of 13 clonal cultures of the dinoflagellate Karlodinium veneficum that were previously isolated from 5 geographic origins and have been maintained in our laboratory from several months to more than 14 years. The total recovered fungal microbiome, which consisted of 349 ASVs (amplicon sequencing variants, sequences clustered at a 100% sequence identity), could be assigned to 4 phyla, 18 classes, 37 orders, 65 families, 97 genera, and 131 species. The fungal consortium displayed high diversity and was dominated by filamentous fungi and ascomycetous and basidiomycetous yeasts. A core set of three genera among all the detected fungi was constitutively present in the K. veneficum strains isolated from geographically distant regions, with the top two most abundant genera, Thyridium and Pseudeurotium, capable of using hydrocarbons as the sole or major source of carbon and energy. In addition, fungal taxa previously documented as endophytes in other hosts were also found in all tested strains of K. veneficum. Because host–endophyte interactions are highly variable and strongly case-dependent, these fungal taxa were not necessarily genuine endosymbionts of K. veneficum; instead, it raised the possibility that dinoflagellates could potentially serve as an alternative ecological niche for the colonization of fungal endophytes. Our findings lay the foundation for further investigations into the potential roles or functions of fungi in the regulation of the growth dynamics and HABs of marine dinoflagellates in the field.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Different Geographic Strains of Dinoflagellate Karlodinium veneficum Host Highly Diverse Fungal Community and Potentially Serve as Possible Niche for Colonization of Fungal Endophytes

Deng

Wang

et al. 2023

IJMS

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“…[17][18][19] Research on beneficial microorganisms with the aim of unlocking potential applications for enhancing crop productivity and protection has increased in recent years. 20,21 Despite the growing number of commercially available microbial biofertilizers and biostimulants, the number of microbial biocontrol products is still small compared to the conventional synthetic product market. [22][23][24][25] Van Lenteren et al 26 identified just over 200 registered microbial biocontrol products, representing 57 different genera, used for augmentative biocontrol in different parts of the world.…”

Section: Introductionmentioning

confidence: 99%

Endophytic Beauveria bassiana induces biosynthesis of flavonoids in oilseed rape following both seed inoculation and natural colonization

Muola,

Birge,

Helander

et al. 2023

Pest Management Science

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BackgroundCultivation of oilseed rape Brassica napus is pesticide‐intensive, and alternative plant protection strategies are needed because both pesticide resistance and legislation narrow the range of effective chemical pesticides. The entomopathogenic fungus Beauveria bassiana is used as a biocontrol agent against various insect pests, but little is known about its endophytic potential and role in plant protection for oilseed rape. First, we studied whether B. bassiana can establish as an endophyte in oilseed rape, following seed inoculation. To evaluate the plant protection potential of endophytic B. bassiana on oilseed rape, we next examined its ability to induce plant metabolite biosynthesis. In another experiment, we tested the effect of seed inoculation on seedling survival in a semi‐field experiment.ResultsBeauveria bassiana endophytically colonized oilseed rape following seed inoculation, and, in addition, natural colonization was also recorded. Maximum colonization rate was 40%, and generally increased with inoculation time. Seed inoculation did not affect the germination probability or growth of oilseed rape, but B. bassiana inoculated seeds germinated more slowly compared to controls. Endophytic colonization of B. bassiana induced biosynthesis of several flavonoids in oilseed rape leaves under controlled conditions. In the experiment conducted in semi‐field conditions, inoculated seedlings had slightly higher mortality compared to control seedlings.ConclusionBeauveria bassiana showed endophytic potential on oilseed rape via both natural colonization and seed inoculation, and it induced the biosynthesis of flavonoids. However, its use as an endophyte for plant protection against pests or pathogens for oilseed rape remains unclear. © 2023 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

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“…They may enter the tissue of their hosts, colonize them, and offer affirmative impacts ( Ray et al, 2017 ). Fungal endophytic colonization induces physiological changes and modifies gene expression in the plants, thereby uplifting plant productivity via higher photosynthesis rate, promoting the shoots and roots growth, enhancing uptake and nutrient use efficiency conferring abiotic (drought, salinity, high temperature, high CO 2 , and metal toxicity) and biotic stress (pathogens and pests) tolerance ( Harman and Uphoff, 2019 ; Grabka et al, 2022 ; Liu-Xu et al, 2022 ). Many fungal endophytes have been reported and extracted from healthy plants potent enough to be incorporated artificially as biofertilizers and biocontrol agents in crops ( Busby et al, 2016 ; Bastami et al, 2021 ; Grabka et al, 2022 ).…”

Section: Introductionmentioning

confidence: 99%

Fungal Endophytes to Combat Biotic and Abiotic Stresses for Climate-Smart and Sustainable Agriculture

Verma

Shameem²,

Jatav

et al. 2022

Front. Plant Sci.

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The agricultural sustainability concept considers higher food production combating biotic and abiotic stresses, socio-economic well-being, and environmental conservation. On the contrary, global warming-led climatic changes have appalling consequences on agriculture, generating shifting rainfall patterns, high temperature, CO2, drought, etc., prompting abiotic stress conditions for plants. Such stresses abandon the plants to thrive, demoting food productivity and ultimately hampering food security. Though environmental issues are natural and cannot be regulated, plants can still be enabled to endure these abnormal abiotic conditions, reinforcing the stress resilience in an eco-friendly fashion by incorporating fungal endophytes. Endophytic fungi are a group of subtle, non-pathogenic microorganisms establishing a mutualistic association with diverse plant species. Their varied association with the host plant under dynamic environments boosts the endogenic tolerance mechanism of the host plant against various stresses via overall modulations of local and systemic mechanisms accompanied by higher antioxidants secretion, ample enough to scavenge Reactive Oxygen Species (ROS) hence, coping over-expression of defensive redox regulatory system of host plant as an aversion to stressed condition. They are also reported to ameliorate plants toward biotic stress mitigation and elevate phytohormone levels forging them worthy enough to be used as biocontrol agents and as biofertilizers against various pathogens, promoting crop improvement and soil improvement, respectively. This review summarizes the present-day conception of the endophytic fungi, their diversity in various crops, and the molecular mechanism behind abiotic and biotic resistance prompting climate-resilient aided sustainable agriculture.

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Advances in endophytic fungi research: a data analysis of 25 years of achievements and challenges

Cited by 17 publications

References 417 publications

Different Geographic Strains of Dinoflagellate Karlodinium veneficum Host Highly Diverse Fungal Community and Potentially Serve as Possible Niche for Colonization of Fungal Endophytes

Different Geographic Strains of Dinoflagellate Karlodinium veneficum Host Highly Diverse Fungal Community and Potentially Serve as Possible Niche for Colonization of Fungal Endophytes

Endophytic Beauveria bassiana induces biosynthesis of flavonoids in oilseed rape following both seed inoculation and natural colonization

Fungal Endophytes to Combat Biotic and Abiotic Stresses for Climate-Smart and Sustainable Agriculture

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