Advances in electron microscopy: A qualitative view of instrumentation development for macromolecular imaging and tomography

Cryo electron microscopy to determine the structure of macromolecular complexes

“…The recent, spectacular advance in resolution of single particle cryo EM is mainly due to the development of direct electron detectors [29], [30], [31], [32]. The effect on the EM field is similar to the effect on macromolecular crystallography of the replacement of photographic film with electronic detectors for X-rays.…”

Electron microscopy: the coming of age of a structural biology technique

“…Rasmus Schroeder discusses the importance of improved instrumentation for successful EM; the incorporation of energy filters, aberration correctors and phase plates, among other advances, has led to higher quality data [3]. Together with automation procedures and (most important) new recording devices, this awakens expectations for considerable advances in the near future.…”

Calculation of Small-Angle Scattering Patterns

“…Imaging techniques, when applicable, are very useful since they can provide a direct indication of the shape and size of the investigated system. Electron microscopy (EM) methods like transmission electron microscopy (TEM) and scanning electron microscopy (SEM) give important information on the structures in high resolution [4][5][6]. However, these methods demand the use of special experimental conditions like measurements in vacuum and the use of coating agents.…”