Advances in electrochemiluminescence for single-cell analysis

Abstract

In this review, we summarise the device design strategies and the recent development in single-cell ECL analysis systems (sensing and imaging systems), as well as the modification of ECL probes through different functionalization strategies.

A bimodal time-gated luminescence–magnetic resonance imaging nanoprobe based on a europium(iii) complex anchored on BSA-coated MnO2 nanosheets for highly selective detection of H2O2

“…Due to the high reactivity of GSH towards MnO 2 nanosheets, the H 2 O 2 nanoprobes based on MnO 2 nanosheets may suffer from interference from GSH. 27 To prevent the decomposition of MnO 2 nanostructures caused by GSH, Tan et al employed a protective approach involving the coating of the nanostructures with mesoporous silica and the subsequent capping with a gelatin nanolayer. 34 Inspirited by the above strategy, we hypothesize that MnO 2 nanosheets coated with biomacromolecules can resist the corrosion caused by GSH and maintain their reactivity towards H 2 O 2 .…”

“…39 In addition, in contrast to most luminescent lanthanide complexes that require excitation by ultraviolet light, the visible-light-excitable [Eu(BTD) 3 (DPBT)] offers the advantage of avoiding potential damage to biological samples caused by ultraviolet light. 27 Initially, transmission electron microscopy (TEM) and atomic force microscopy (AFM) were applied to study the structure and morphology of the as-prepared BSA@MnO 2 nanosheets. As shown in Fig.…”

“…25,26 In our previous report, H 2 O 2 and GSH exhibited almost identical reactivity towards MnO 2 nanosheets. 27 Given that the GSH content in the cell and body is much higher than that of H 2 O 2 , these H 2 O 2 probes would suffer from the intense interference from GSH when they are used for imaging H 2 O 2 in live cells and tissues. However, there is a lack of experimental data supporting the ability of these H 2 O 2 probes to withstand interference from GSH, and their application for bimodal FI-MRI of H 2 O 2 in vivo was not reported.…”

“…32,33 In previous work, a GSH/H 2 O 2 -responsive nanoprobe based on nanoarchitectures of Eu 3+ complexes anchored on MnO 2 nanosheets, denoted as [Eu(BTD) 3 (DPBT)]@MnO 2 , was developed for TGLI-MRI detection of GSH and H 2 O 2 . 27 However, [Eu(BTD) 3 (DPBT)]@MnO 2 lacks the ability to differentiate between GSH and H 2 O 2 . In this work, to improve the selectivity of this probe for detecting H 2 O 2 , we have synthesized bovine serum albumin (BSA)-coated MnO 2 nanosheets (BSA@MnO 2 ) which were further modified with visible-light-excitable Eu 3+ complexes.…”

Highly sensitive detection of circulating tumour cells based on an ASV/CV dual-signal electrochemical strategy

“…[29][30][31] Depending on the excellent electrochemical and photophysical characteristics of QDs, the introduction of QDs can provide an increase in sensitivity and response speed in electrochemical sensors. 31,32 Among them, cadmium sulde (CdS) has found applications in various elds with a bulk band gap value of ∼2.42 eV. CdS QDs have also been widely reported to prepare CdS QD/polystyrene (PS) composites for electrochemical detection due to PS's low specic weight, biocompatibility, exibility, and high chemical resistance.…”