Advances in capillary electrophoresis

Deyl, Z.; Mikšı́k, Ivan; Tagliaro, Franco

doi:10.1016/s0379-0738(98)00011-5

Cited by 32 publications

(11 citation statements)

References 193 publications

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“…Liquid chromatography techniques (e.g., ion exchange, size exclusion, affinity, and reversed-phase), as well as electrophoretic separation in liquid-phase techniques (capillary isoelectrofocusing (IEF), capillary zone and free-flow electrophoresis (FFE)) are well known methods of protein separation and extensively described in the literature [18][19][20][21][22][23][24]. Each type of LC has already undergone numerous developments and improvements.…”

Section: Liquid Chromatography As a Methods Of Protein Separationmentioning

confidence: 99%

Role of chromatographic techniques in proteomic analysis

Neverova

Eyk

2005

Journal of Chromatography B

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Section: Liquid Chromatography As a Methods Of Protein Separationmentioning

confidence: 99%

Role of chromatographic techniques in proteomic analysis

Neverova

Eyk

2005

Journal of Chromatography B

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“…Both fast and efficient separation can be achieved by using short separation channels, high field strength, and a small volume sample plug. The efficiency of micro CE is comparable with that of the conventional CE but the analysis time is cut down to tens of seconds as compared to half an hour with the standard device [44] . By properly designing the separation channels or coupling CE with another separation technique, like chromatography, a number of 2D CE chips have been reported [45,46] , which can substantially increase the separation resolution.…”

Section: Separationmentioning

confidence: 99%

Micro/Nano Fluidics Mechanics and Transducers

Tai

2012

Microsystems and Nanotechnology

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In biochemical analyses, sample fluid volume in the nano or pico liter range is commonly encountered in various processes. For example, the preparation, mixing, separation, and concentration of cells and biomolecules in such small amount of fluids are very often needed in most analyses. From the science and technology point of view, efficient momentum and energy transfers of the desired fluid and particle motions in such small scales need sufficient comprehension of mechanics and MEMS (micro-electro-mechanical systems) transducers of similar length scales. Interestingly, MEMS/NEMS technologies do enable us to match the device and the fluid length scale for handling fluids in extremely small volume. This opens up a tremendous opportunity for research and development. In traditional fluid dynamics, the flow length scale is much larger than the molecular length scale. Continuum mechanics is the most common hypothesis used for flow research. In the case of micro/nano engineering systems, however, one may encounter regimes from continuum all the way down to molecule-dominated conditions. Therefore, in micro/nano fluidics, there are many new challenges that are very different from those in traditional fluidic systems. In this chapter, we discuss these special issues related to micro/nano fluidics and MEMS transducers in handling extremely small amount of fluids, including embedded particles.Keywords Micro-electro-mechanical-systems (MEMS), nano sciences and technologies, sensors and actuators, microfluidics and nanofluidics, micro/Nano systems et al., Microsystems and Nanotechnology

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“…The effect made the detection limit of HT decrease by about 10-fold. However, the stacking efficiency is poor for neutral or anion substances since their retention factors are near zero [30].…”

Section: Effect Of Injection Mode and Injection Timementioning

confidence: 99%

Micellar electrokinetic capillary chromatography for fast separation and sensitive determination of melatonin and related indoleamines using end‐column amperometric detection

Zhang

et al. 2006

Electrophoresis

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MEKC was used in conjunction with end-column amperometric detection (AD) at a carbon disc electrode (0.3 mm diameter) for the selective and sensitive determination of melatonin and its five related indoleamines including its precursors and metabolites in the pineal gland. The introduction of a sample stacking technique in injection and the buffer additive SDS in the buffer solution system provided the rapid and sensitive analysis. Optimal buffer conditions (10 mmol/L phosphate containing 20 mmol/L SDS, pH 7.2), detection potential (+1.0 V vs. Ag/AgCl), and electrokinetic injection 10 s with the separation voltage of 24 kV were employed to achieve the baseline separation of six pineal hormones within 15 min. The peak currents and the analyte concentrations have a good linear relationship over the range of 6.0 x 10(-8) 6.0 x 10(-5 )mol/L. The detection limits for six pineal hormones by AD are 9.7 to 41.8 nmol/L (equal to 2.0 to 9.7 ng/mL) (S/N = 3), respectively. It is proved to provide about 30- to 250-fold improvement over UV, and be comparable with the sensitive fluorescence detection, which needs pre-column derivatization. The proposed method has been applied for analysis of melatonin and related indoleamines in rat pineal glands. A very simple sample pretreatment procedure, merely involving the homogenization step in perchloric acid, was enough to achieve recoveries in the range of 71 to 127% for all the analytes in the pineal gland.

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Advances in capillary electrophoresis

Cited by 32 publications

References 193 publications

Role of chromatographic techniques in proteomic analysis

Role of chromatographic techniques in proteomic analysis

Micro/Nano Fluidics Mechanics and Transducers

Micellar electrokinetic capillary chromatography for fast separation and sensitive determination of melatonin and related indoleamines using end‐column amperometric detection

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