Advances in Cadaverine Bacterial Production and Its Applications

Ma, Wei; Chen, Kequan; Li, Yan; Hao, Ning; Wang, Xin; Ouyang, Pingkai

doi:10.1016/j.eng.2017.03.012

Cited by 91 publications

(57 citation statements)

References 105 publications

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“…The design of such drugs will now be facilitated by the knowledge that the physiological substrates are short-chain polyamines. In addition to functions in human health, diamines, including cadaverine and putrescine examined in this study, have a range of industrial uses, especially as precursors of polymers related to nylon (20). The discovery that AceI is a secondary transport system for these compounds adds the potential for developing new biological platforms for their largescale biotechnological production based around AceI-mediated efflux, which would provide much needed "green" alternatives to the petroleum-based precursors currently produced (20).…”

Section: Discussionmentioning

confidence: 98%

Short-chain diamines are the physiological substrates of PACE family efflux pumps

Hassan

Naidu

Edgerton

et al. 2019

Proc. Natl. Acad. Sci. U.S.A.

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Acinetobacter baumannii has rapidly emerged as a major cause of gram-negative hospital infections worldwide. A. baumannii encodes for the transport protein AceI, which confers resistance to chlorhexidine, a widely used antiseptic. AceI is also the prototype for the recently discovered proteobacterial antimicrobial compound efflux (PACE) family of transport proteins that confer resistance to a range of antibiotics and antiseptics in many gram-negative bacteria, including pathogens. The gene encoding AceI is conserved in the core genome of A. baumannii, suggesting that it has an important primordial function. This is incongruous with the sole characterized substrate of AceI, chlorhexidine, an entirely synthetic biocide produced only during the last century. Here we investigated a potential primordial function of AceI and other members of the PACE family in the transport of naturally occurring polyamines. Polyamines are abundant in living cells, where they have physiologically important functions and play multifaceted roles in bacterial infection. Gene expression studies revealed that the aceI gene is induced in A. baumannii by the short-chain diamines cadaverine and putrescine. Membrane transport experiments conducted in whole cells of A. baumannii and Escherichia coli and also in proteoliposomes showed that AceI mediates the efflux of these short-chain diamines when energized by an electrochemical gradient. Assays conducted using 8 additional diverse PACE family proteins identified 3 that also catalyze cadaverine transport. Taken together, these results demonstrate that short-chain diamines are common substrates for the PACE family of transport proteins, adding to their broad significance as a novel family of efflux pumps.

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Section: Discussionmentioning

confidence: 98%

Short-chain diamines are the physiological substrates of PACE family efflux pumps

Hassan

Naidu

Edgerton

et al. 2019

Proc. Natl. Acad. Sci. U.S.A.

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“…Because of the spontaneous fermentation which is likely occurring in the feed troughs, bacterial degradation of AA can potentially occur with the resultant formation of biogenic amines, high levels of which are toxic 27,28,36,46 . Cadaverine and putrescine are biogenic amines formed by the decarboxylation of lysine 47,48 . In this study, only the cereal fraction of the diet was soaked in order to minimise the time that synthetic AA (included in the balancer fraction) were in contact with water prior to feeding.…”

Section: Discussionmentioning

confidence: 99%

Effect of cereal soaking and carbohydrase supplementation on growth, nutrient digestibility and intestinal microbiota in liquid-fed grow-finishing pigs

Torres-Pitarch

Gardiner

Cormican

et al. 2020

Sci Rep

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Soaking the cereal fraction of a liquid diet prior to feeding (C soak), and/or carbohydrase enzyme supplementation (ENZ) are likely to modulate both feed and intestinal microbial populations and improve feed efficiency (FE) in pigs. To test this hypothesis, a total of 392 grow-finisher pigs (~33.4 kg, 7 pigs/pen) were randomly allocated to 4 treatments in a 2 × 2 factorial arrangement for 70 days as follows: (1) fresh liquid feed (Fresh); (2) Cereal soaked liquid feed (Soak); (3) Fresh + ENZ and (4) Soak + ENZ. An interaction between ENZ and C soak was found for average daily gain (ADG) during the growing phase (day 0 to 21; P < 0.05) where pigs fed the Soak + ENZ diet had higher ADG than pigs fed the Fresh + ENZ diet. No treatment effect was found for ADG thereafter. Enzyme supplementation increased total tract nutrient digestibility (P < 0.05) and reduced caecal VFA concentrations (P < 0.05) but did not improve pig growth or FE. Both C soak and ENZ modulated intestinal microbiota composition; increasing abundance of bacterial taxa that were negatively correlated with pig growth and reducing abundance of taxa positively correlated with pig growth and caecal butyrate concentration. In conclusion, both strategies (C soak and ENZ) improved nutrient digestibility in pigs and modulated intestinal microbiota composition.

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“…In a number of bacteria, including many associated with the microbiota (Table S5), cadaverine is encoded by both the constitutive ldc gene cluster as well as the cad gene cluster, which is induced at low pH (pH <6.8). [29] High level production of cadaverine by the CadA lysine decarboxylase plays a role in protecting against acid stress. [30].…”

Section: Known and Novel Ligands For Neurotransmitter Receptorsmentioning

confidence: 99%

Mapping interactions of microbial metabolites and human receptors

et al. 2019

Preprint

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Despite evidence linking the human microbiome to health and disease, the mechanistic details of how the microbiota affects human physiology remain largely unknown. Metabolites encoded by bacteria are expected to play an integral role in the microbiota’s effect on its human host. Assigning function to these metabolites is therefore critical to determining the molecular underpinnings of the host-microbe relationship and ultimately developing microbiota inspired therapies. Here we use large-scale functional screening of small molecules produced by individual members of a simplified human microbiota to identify bacterial metabolites that agonize G-protein coupled receptors (GPCR). This analysis revealed a complex network of metabolite host receptor interactions and guided our identification of multiple microbiota derived agonists of GPCRs associated with diverse biological functions within the nervous and immune systems, among others. Collectively, the metabolite-receptor pairs we uncovered indicate that diverse aspects of human health are potentially modulated by structurally simple metabolites arising from primary bacterial metabolism.Statement of SignificanceBacteria residing within the human body have been shown to influence human health. It is likely that physiological responses to the human microbiota are mediated by the collection of small molecules encoded within these bacteria. In this study we use direct functional screening of small molecules produced by individual members of a simplified human microbiota to identify new G protein coupled receptor-metabolite interactions that seek to explain the molecular underpinnings of the microbiota’s influence on its human host.

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Advances in Cadaverine Bacterial Production and Its Applications

Cited by 91 publications

References 105 publications

Short-chain diamines are the physiological substrates of PACE family efflux pumps

Short-chain diamines are the physiological substrates of PACE family efflux pumps

Effect of cereal soaking and carbohydrase supplementation on growth, nutrient digestibility and intestinal microbiota in liquid-fed grow-finishing pigs

Mapping interactions of microbial metabolites and human receptors

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