Advances in Buffalo Bull Fertility Prediction

Kumaresan, A.; Paul, Nilendu; Aslam, Muhammad; Nag, Pranab Kumar

doi:10.1007/978-981-16-7531-7_9

Cited by 1 publication

(2 citation statements)

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“…Like in case of cattle, several thousands of buffaloes are artificially inseminated using semen from a single bull; therefore, bull fertility is an important determinant of successful reproduction. On the other hand, buffalo bull fertility has not been understood properly; only very few studies are available on sperm molecular health and bull fertility in this species 7 . In the present study, we carried out an in-depth proteome profiling of buffalo bull spermatozoa and identified potential fertility associated sperm proteins that could serve as marker for selection of high-fertile buffalo bulls for artificial breeding.…”

Section: Discussionmentioning

confidence: 99%

“…Both male and female contribute to conception, however, the role of males is amplified in artificial insemination because one bull is used to artificially bred several thousands of females. Buffalo spermatozoa are unique in terms of structural, compositional and functional attributes, and thus the fertility prediction methods/tools used in cattle may not hold good for this species 6 , 7 . Insemination of females using semen from low-fertile bulls causes significant loss to the dairy industry 8 , 9 .…”

Section: Introductionmentioning

confidence: 99%

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Establishment of a repertoire of fertility associated sperm proteins and their differential abundance in buffalo bulls (Bubalus bubalis) with contrasting fertility

Kumaresan

Sinha²,

Paul³

et al. 2023

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Sperm harbours a wide range of proteins regulating their functions and fertility. In the present study, we made an effort to characterize and quantify the proteome of buffalo bull spermatozoa, and to identify fertility associated sperm proteins through comparative proteomics. Using high-throughput mass spectrometry platform, we identified 1305 proteins from buffalo spermatozoa and found that these proteins were mostly enriched in glycolytic process, mitochondrial respiratory chain, tricarboxylic acid cycle, protein folding, spermatogenesis, sperm motility and sperm binding to zona pellucida (p < 7.74E−08) besides metabolic (p = 4.42E−31) and reactive oxygen species (p = 1.81E−30) pathways. Differential proteomic analysis revealed that 844 proteins were commonly expressed in spermatozoa from both the groups while 77 and 52 proteins were exclusively expressed in high- and low-fertile bulls, respectively. In low-fertile bulls, 75 proteins were significantly (p < 0.05) upregulated and 176 proteins were significantly (p < 0.05) downregulated; these proteins were highly enriched in mitochondrial respiratory chain complex I assembly (p = 2.63E−07) and flagellated sperm motility (p = 7.02E−05) processes besides oxidative phosphorylation pathway (p = 6.61E−15). The down regulated proteins in low-fertile bulls were involved in sperm motility, metabolism, sperm-egg recognition and fertilization. These variations in the sperm proteome could be used as potential markers for the selection of buffalo bulls for fertility.

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Section: Discussionmentioning

confidence: 99%