Advances in breeding and biotechnology of legume crops

Jacob, Cristián; Carrasco, Basilio; Schwember, Andrés R.

doi:10.1007/s11240-016-1106-2

Cited by 38 publications

(18 citation statements)

References 243 publications

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“…As covered in this review, legumes produce a diverse array of secondary metabolites including a large subset of compounds with biopharmaceutical/nutraceutical properties. The production of these phytochemicals can be increase through crop improvement using classical breeding to genetic approaches (Jacob et al, 2016). Legumes with increased health-beneficial secondary metabolites are potential raw materials for producing pharmaceutical products.…”

Section: Molecular Breeding and Secondary Metabolite Contentmentioning

confidence: 99%

“…The genetic variability of legume species is fundamental to identify parental lines to be used in breeding programs and exploit legume secondary metabolites. Modern targeted breeding programs use tools, such as quantitative trait loci, marked-assisted selection, and genomics applications (Collard and Mackill, 2008;Jacob et al, 2016). DNA-based molecular markers are used to characterize genomic regions (insertions, deletions, mutations) controlling a particular trait or gene to differentiate individuals for germplasm identification and characterization (Nadeem et al, 2018).…”

Section: Molecular Breeding and Secondary Metabolite Contentmentioning

confidence: 99%

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The Effects of Domestication on Secondary Metabolite Composition in Legumes

Contador

et al. 2020

Front. Genet.

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Legumes are rich in secondary metabolites, such as polyphenols, alkaloids, and saponins, which are important defense compounds to protect the plant against herbivores and pathogens, and act as signaling molecules between the plant and its biotic environment. Legume-sourced secondary metabolites are well known for their potential benefits to human health as pharmaceuticals and nutraceuticals. During domestication, the color, smell, and taste of crop plants have been the focus of artificial selection by breeders. Since these agronomic traits are regulated by secondary metabolites, the basis behind the genomic evolution was the selection of the secondary metabolite composition. In this review, we will discuss the classification, occurrence, and health benefits of secondary metabolites in legumes. The differences in their profiles between wild legumes and their cultivated counterparts will be investigated to trace the possible effects of domestication on secondary metabolite compositions, and the advantages and drawbacks of such modifications. The changes in secondary metabolite contents will also be discussed at the genetic level to examine the genes responsible for determining the secondary metabolite composition that might have been lost due to domestication. Understanding these genes would enable breeding programs and metabolic engineering to produce legume varieties with favorable secondary metabolite profiles for facilitating adaptations to a changing climate, promoting beneficial interactions with biotic factors, and enhancing health-beneficial secondary metabolite contents for human consumption.

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Section: Molecular Breeding and Secondary Metabolite Contentmentioning

confidence: 99%

Section: Molecular Breeding and Secondary Metabolite Contentmentioning

confidence: 99%

The Effects of Domestication on Secondary Metabolite Composition in Legumes

Contador

et al. 2020

Front. Genet.

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“…Faba bean contains certain harmful compounds that have not been reduced notably via plant breeding [3,4].…”

Section: Introductionmentioning

confidence: 99%

Possibilities of reducing amounts of vicine and convicine in faba bean suspensions and sourdoughs

Pulkkinen

Coda

Lampi

et al. 2019

Eur Food Res Technol

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Vicine and convicine may be removed from faba bean by hydrolysis to the corresponding aglycones, divicine and isouramil. For total elimination of their toxicity, further degradation of the aglycones should be shown. The aim of the study was to investigate hydrolysis of vicine and convicine using the enzymatic activity in faba bean in flour suspensions and selected lactic acid bacteria used as starters for faba bean fermentation. In addition, the effect of acidity on the stability of vicine and convicine was investigated. Sourdoughs were used in a baking process to obtain breads as final products. Vicine, convicine, and their aglycones were analyzed using reversed phase high pressure liquid chromatography with UV detection (RP-HPLC-UV). Incubation of the suspensions showed rather small vicine and convicine losses. Acidity itself did not cause losses under the conditions studied, apart from that of convicine at low pH. In sourdough fermentation with strains of Lactobacillus plantarum and Pediococcus pentosaceus, losses of vicine and convicine were dependent on the fermentation temperature and the β-glucosidase activity of the starter. Compared to fermentation at 20 °C, more intense acidification at 25 °C resulted in decrease of vicine up to 85% and convicine up to 47%. Levels of vicine and convicine in breads were comparable to levels in sourdoughs. Furthermore, the aglycones were not detected from breads.

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“…As many legumes are grown (or have their center of origin) in regions with an arid to semi-arid climate ( Smýkal et al, 2015 ), a number of studies have identified genes, QTLs, ESTs, and SNPs that are responsive to drought stress in several species ( Jacob et al, 2016 ). However, the molecular basis of water stress tolerance is not fully understood in M. truncatula ( Araújo et al, 2015 ).…”

Section: Introductionmentioning

confidence: 99%

PEG Induces High Expression of the Cell Cycle Checkpoint Gene WEE1 in Embryogenic Callus of Medicago truncatula: Potential Link between Cell Cycle Checkpoint Regulation and Osmotic Stress

et al. 2017

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Polyethylene glycol (PEG) can be used to mimic osmotic stress in plant tissue cultures to study mechanisms of tolerance. The aim of this experiment was to investigate the effects of PEG (M.W. 6000) on embryogenic callus of Medicago truncatula. Leaf explants were cultured on MS medium with 2 mg L-1 NAA and 0.5 mg L-1 BAP for 5 months. Then, calli were transferred to the same medium further supplemented with 10% (w/v) 6000 PEG for 6 months in order to study physiological and putative molecular markers of water stress. There were no significant differences in growth rate of callus or mitotic index ± PEG although embryogenic potential of PEG treated callus was morphologically enhanced. Cells were rounder on PEG medium and cell size, nuclear size and endoreduplication increased in response to the PEG treatment. Significant increases in soluble sugar and proline accumulation occurred under PEG treatment compared with the control. Significantly, high MtWEE1 and MtCCS52 expression resulted from 6 months of PEG treatment with no significant differences in MtSERK1 or MtP5CS expression but down regulation of MtSOS expression. The results are consistent in showing elevated expression of a cell cycle checkpoint gene, WEE1. It is likely that the cell cycle checkpoint surveillance machinery, that would include WEE1 expression, is ameliorating the effects of the stress imposed by PEG.

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Advances in breeding and biotechnology of legume crops

Cited by 38 publications

References 243 publications

The Effects of Domestication on Secondary Metabolite Composition in Legumes

The Effects of Domestication on Secondary Metabolite Composition in Legumes

Possibilities of reducing amounts of vicine and convicine in faba bean suspensions and sourdoughs

PEG Induces High Expression of the Cell Cycle Checkpoint Gene WEE1 in Embryogenic Callus of Medicago truncatula: Potential Link between Cell Cycle Checkpoint Regulation and Osmotic Stress

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