Advances in biofabrication techniques towards functional bioprinted heterogeneous engineered tissues: A comprehensive review

Harley, William S.; Li, Chi Chung; Toombs, Joseph; O’Connell, Cathal; Taylor, Hayden; Heath, Daniel E.; Collins, Dave

doi:10.1016/j.bprint.2021.e00147

Cited by 46 publications

(40 citation statements)

References 320 publications

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“…However, extrusion-based printing generally dispenses the cell-laden inks as fibers with a relatively low resolution of ~100 µm, which makes the method unsuitable for single-cell printing [47]. Contrarily, laser-based cell printing has long been utilized in single-cell printing because laser printing can precisely select and position live cells on a predefined location from a cell suspension with high density [71][72][73][74][75]. In laser-based cell printing, cells are pre-loaded on a laser-sensitive surface, and then the laser is applied to transfer the target cells onto an underlying substrate [47].…”

Section: Noncontact Printingmentioning

confidence: 99%

“…However, the time requirement and limited throughput are the major drawbacks of laser-based single-cell printing. Furthermore, the concern of the laser radiation-induced damage on the printed cells, expensive printer components, and complicated setup and operation limit the applications of laser-based cell printing [74][75][76].…”

Section: Noncontact Printingmentioning

confidence: 99%

“…Third, inkjet-like printing is easily scalable for high throughput production with multiple printheads. Finally, inkjet-like printing is environmentally friendly with less raw material waste [47,68,74,[77][78][79].…”

Section: Noncontact Printingmentioning

confidence: 99%

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Advances in Single-Cell Printing

Zhou¹,

Wu²,

Wen³

et al. 2022

Micromachines

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Single-cell analysis is becoming an indispensable tool in modern biological and medical research. Single-cell isolation is the key step for single-cell analysis. Single-cell printing shows several distinct advantages among the single-cell isolation techniques, such as precise deposition, high encapsulation efficiency, and easy recovery. Therefore, recent developments in single-cell printing have attracted extensive attention. We review herein the recently developed bioprinting strategies with single-cell resolution, with a special focus on inkjet-like single-cell printing. First, we discuss the common cell printing strategies and introduce several typical and advanced printing strategies. Then, we introduce several typical applications based on single-cell printing, from single-cell array screening and mass spectrometry-based single-cell analysis to three-dimensional tissue formation. In the last part, we discuss the pros and cons of the single-cell strategies and provide a brief outlook for single-cell printing.

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Section: Noncontact Printingmentioning

confidence: 99%

Section: Noncontact Printingmentioning

confidence: 99%

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Advances in Single-Cell Printing

Zhou¹,

Wu²,

Wen³

et al. 2022

Micromachines

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“…Cartilage and bone tissue engineering has been pursued since the 1990s, with most developed approaches based on highly simplistic, monolithic representations of each tissue type. In recent years, additive fabrication technologies have emerged, providing new tools for engineering living tissues, including bone and cartilage [22,23]. This adoption of additive fabrication techniques for the regeneration of living tissues has created the new subfield termed 'biofabrication' [24].…”

Section: Introductionmentioning

confidence: 99%

3D Printed Multiphasic Scaffolds for Osteochondral Repair: Challenges and Opportunities

Doyle

Snow

Duchi

et al. 2021

IJMS

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Osteochondral (OC) defects are debilitating joint injuries characterized by the loss of full thickness articular cartilage along with the underlying calcified cartilage through to the subchondral bone. While current surgical treatments can provide some relief from pain, none can fully repair all the components of the OC unit and restore its native function. Engineering OC tissue is challenging due to the presence of the three distinct tissue regions. Recent advances in additive manufacturing provide unprecedented control over the internal microstructure of bioscaffolds, the patterning of growth factors and the encapsulation of potentially regenerative cells. These developments are ushering in a new paradigm of ‘multiphasic’ scaffold designs in which the optimal micro-environment for each tissue region is individually crafted. Although the adoption of these techniques provides new opportunities in OC research, it also introduces challenges, such as creating tissue interfaces, integrating multiple fabrication techniques and co-culturing different cells within the same construct. This review captures the considerations and capabilities in developing 3D printed OC scaffolds, including materials, fabrication techniques, mechanical function, biological components and design.

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“…Biofabrication combines cells, biomaterials and biological factors-collectively known as bioinks-and delivers them in a precise pattern to recapitulate elements of heterogeneous tissues [22][23][24]. Although biofabrication has made a remarkable impact in areas of tissue engineering and drug delivery, its application and rapid progress into the clinic has been limited, firstly, by the lack of suitable biomaterials and bioinks [25,26].…”

Section: Introductionmentioning

confidence: 99%

Biofabrication Strategies for Musculoskeletal Disorders: Evolution towards Clinical Applications

et al. 2021

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Biofabrication has emerged as an attractive strategy to personalise medical care and provide new treatments for common organ damage or diseases. While it has made impactful headway in e.g., skin grafting, drug testing and cancer research purposes, its application to treat musculoskeletal tissue disorders in a clinical setting remains scarce. Albeit with several in vitro breakthroughs over the past decade, standard musculoskeletal treatments are still limited to palliative care or surgical interventions with limited long-term effects and biological functionality. To better understand this lack of translation, it is important to study connections between basic science challenges and developments with translational hurdles and evolving frameworks for this fully disruptive technology that is biofabrication. This review paper thus looks closely at the processing stage of biofabrication, specifically at the bioinks suitable for musculoskeletal tissue fabrication and their trends of usage. This includes underlying composite bioink strategies to address the shortfalls of sole biomaterials. We also review recent advances made to overcome long-standing challenges in the field of biofabrication, namely bioprinting of low-viscosity bioinks, controlled delivery of growth factors, and the fabrication of spatially graded biological and structural scaffolds to help biofabricate more clinically relevant constructs. We further explore the clinical application of biofabricated musculoskeletal structures, regulatory pathways, and challenges for clinical translation, while identifying the opportunities that currently lie closest to clinical translation. In this article, we consider the next era of biofabrication and the overarching challenges that need to be addressed to reach clinical relevance.

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Advances in biofabrication techniques towards functional bioprinted heterogeneous engineered tissues: A comprehensive review

Cited by 46 publications

References 320 publications

Advances in Single-Cell Printing

Advances in Single-Cell Printing

3D Printed Multiphasic Scaffolds for Osteochondral Repair: Challenges and Opportunities

Biofabrication Strategies for Musculoskeletal Disorders: Evolution towards Clinical Applications

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