Advances in articular cartilage repair

Beris, Alexandros E.; Lykissas, Marios G.; Papageorgiou, Christos D.; Georgoulis, Anastasios D.

doi:10.1016/j.injury.2005.10.007

Cited by 120 publications

(80 citation statements)

References 79 publications

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“…Currently, the source of the odontoblasts has been an obstacle to study tooth repair and tooth regeneration. In addition, large clinical defects of bone or cartilage have often limited potential for repair, and hence, large lesions never heal spontaneously in orthopedic patients [Beris et al, 2005]. Stem cell therapy is one of the most promising methods for tooth tissue engineering because the transplantation of materials containing odontogenic stem cells provides an excellent inductive means to regenerate new tooth tissues [Smith and Leaver, 1979;Begue-Kirn et al, 1992;Young et al, 2002;Shi and Gronthos, 2003;Ohazama et al, 2004].…”

Section: Discussionmentioning

confidence: 99%

Characterization of Ectomesenchymal Cells Isolated from the First Branchial Arch during Multilineage Differentiation

Yan

Lin²,

Jiao³

et al. 2006

Cells Tissues Organs

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Ectomesenchymal cells isolated from the first branchial arch have the potential to differentiate into a variety of cell lineages both in vitro and in vivo. This study was aimed to confirm the plasticity of multilineage differentiation with molecular and cellular characterization. Monolayer cultures of ectomesenchymal cells harvested from the first branchial arch primordia in embryonic day 9.5 BALB/c mice were passaged 3 times before analysis. Staining with antibodies against S-100, p75 and vimentin suggested that the population of stem cells originated from ectomesenchyme, with few contaminating cells stained for cytokeratin. Then, cells were transferred to adipogenic, osteogenic, chondrogenic and odontogenic media. The initiation of controlled differentiation was determined with histological assays, and the expression of tissue-specific genes was detected using immunocytochemical staining and reverse transcription polymerase chain reaction. The adipogenic ectomesenchymal cells showed accumulation of lipid vacuoles and expression of lipoprotein lipase and peroxisome proliferator-activated receptor γ₂. Following osteoinduction, the fibroblast-like cells became cuboidal and formed mineralized nodules. In addition, the expression of mRNA encoding osteocalcin and osteopontin proved osteogenesis at the molecular level. Chondrogenic lineage expressed collagen type II, aggrecan and Sox9 with a low level of collagen type I in monolayer culture. Odontogenesis was determined by dentin sialophosphoprotein, collagen type I and dentin matrix protein 1 expression. Therefore, we have demonstrated that ectomesenchymal cells from the first branchial arch are capable of extensive multilineage differentiation in vitro, controllable by the culture environment. This makes them a relevant and valuable source of stem cells for research of craniofacial development and tissue engineering of restoration.

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Section: Discussionmentioning

confidence: 99%

Characterization of Ectomesenchymal Cells Isolated from the First Branchial Arch during Multilineage Differentiation

Yan

Lin²,

Jiao³

et al. 2006

Cells Tissues Organs

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“…An ideal osteochondral graft substitute must (i) be biocompatible, (ii) provide adequate mechanical properties to withstand the implantation procedure and the subsequent mechanical and hydrodynamic loading within the joint, (iii) possess sufficiently high levels of porosity to allow ingrowth of host tissue and/or seeded cells, (iv) be retained at the site of implantation (Beris, 2005), (v) support and direct regeneration of the two predominant tissue types, namely bone and cartilage (Hutmacher, 2000) to ensure overlying repair cartilage is adequately supported, (vi) be optimised for cell attachment to favour colonisation by native cells (Coutts et al, 2001), (vii) promote integration with the existing tissues (Beris, 2005). Composition is elementary in creating biologically active materials that can induce synthesis of new tissue in vitro and in vivo.…”

Section: Wwwintechopencommentioning

confidence: 99%

Composite Scaffolds for Orthopaedic Regenerative Medicine

Gleeson¹,

O’Brien²

2011

Advances in Composite Materials for Medicine and Nanotechnology

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“…Lesions in articular cartilage evolving from traumatic or pathological processes represent a very common clinical condition in both developed and developing countries, and show increasing prevalence due to the steady prolongation of life expectancy (Beris et al, 2005). During the last decades biological repair of cartilage has been attempted by both the direct implantation of autologous cells, (Brittberg, 2008) and by the ex-vivo generation of tissue engineering-based implants (Filardo et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Mesenchymal stromal cells from human umbilical cords display poor chondrogenic potential in scaffold-free three dimensional cultures

Islam

Hansen²,

Mennan³

et al. 2016

eCM

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Many researchers world over are currently investigating the suitability of stromal cells harvested from foetal tissues for allogeneic cell transplantation therapies or for tissue engineering purposes. In this study, we have investigated the chondrogenic potential of mesenchymal stromal cells (MSCs) isolated from whole sections of human umbilical cord or mixed cord (UCSCs-MC), and compared them with cells isolated from synovial membrane (SMSCs), Hoffa's fat pad (HFPSCs) and cartilage. All MSCs were positive for surface markers including CD73, CD90, CD105, CD44, CD146 and CD166, but negative for CD11b, CD19, CD34, CD45 and HLA-DR in addition to CD106 and CD271. Chondrogenic potential of all cell sources was studied using 3D pellet cultures incubated in the presence of different combinations of anabolic substances such as dexamethasone, IGF-1, TGF-β1, TGF-β3, BMP-2 and BMP-7. BMP-2 and dexamethasone in combination with TGF-β1 or TGF-β3 excelled at inducing chondrogenesis on SMSCs, HFPSCs and chondrocytes, as measured by glycosaminoglycans and collagen type II staining of pellets, quantitative glycosaminoglycan expression, quantitative PCR of cartilage signature genes and electron microscopy. In contrast, none of the tested growth factor combinations was sufficient to induce chondrogenesis on UCSCs-MC. Moreover, incubation of UCSCs-MC spheroids in the presence of cartilage pieces or synovial cells in co-cultures did not aid chondrogenic induction. In summary, we show that in comparison with MSCs harvested from adult joint tissues, UCSCs-MC display poor chondrogenic abilities. This observation should alert researchers at the time of considering UCSCs-MC as cartilage forming cells in tissue engineering or repair strategies.

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Advances in articular cartilage repair

Cited by 120 publications

References 79 publications

Characterization of Ectomesenchymal Cells Isolated from the First Branchial Arch during Multilineage Differentiation

Characterization of Ectomesenchymal Cells Isolated from the First Branchial Arch during Multilineage Differentiation

Composite Scaffolds for Orthopaedic Regenerative Medicine

Mesenchymal stromal cells from human umbilical cords display poor chondrogenic potential in scaffold-free three dimensional cultures

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