Advances in Aptamer-Based Biosensors and Cell-Internalizing SELEX Technology for Diagnostic and Therapeutic Application

Gan, Zixuen; Roslan, Muhamad Aidilfitri Mohamad; Shukor, Mohd Yunus; Halim, Murni; Yasid, Nur Adeela; Abdullah, Jaafar; Yasin, Ina Salwany Md; Wasoh, Helmi

doi:10.3390/bios12110922

Cited by 18 publications

(20 citation statements)

References 102 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Aptamer-based biosensors.-Innovative analytical tools known as "aptamer-based biosensors" use the special binding abilities of aptamers to identify and measure target molecules. 65 An observable signal is produced by the aptamer's conformational change upon binding to the target molecule in a sample . 10 After that, this signal is converted into a quantifiable output, which may involve modifications to its electrical, optical, or chemical characteristics.…”

Section: Innovations In Biosensing Technologiesmentioning

confidence: 99%

Review—Aptamers and Biomimetic Receptors in Biosensing: Innovations and Applications

Tripathi,

Pandey,

Mishra

et al. 2024

J. Electrochem. Soc.

View full text Add to dashboard Cite

The fields of biosensing have been transformed by the discovery of extraordinary molecular recognition components, such as aptamers and biomimetic receptors. Systematic Evolution of Ligands by Exponential Enrichment (SELEX) is a method used to select aptamers, or short sequences of single-stranded DNA (ssDNA) or RNA (ssRNA), based on their unique binding affinity to target molecules. Molecularly imprinted polymers (MIPs) are a type of biomimetic receptor that mimics the selectivity of natural receptors inside a synthetic matrix. They make it possible to identify pathogens, and illness biomarkers with accuracy. Aptamers and biomimetic receptors play crucial roles in various fields including diagnostics, therapeutics, and biosensing. Their high specificity, versatility, and adaptability enable targeted detection, drug delivery, and biomolecule manipulation, thereby contributing to advancements in personalized medicine, biotechnology, and disease diagnosis. Aptamers and biomimetic receptors have been combined with cutting-edge technologies, like nanotechnology and lab-on-a-chip systems, to create biosensors that are quick, portable, and extremely sensitive. These recognition features are anticipated to become more important as technology develops, helping to address global issues, advance biosensing capabilities, and raise people's standard of living everywhere. Recent advancements and innovation on Aptamers and Biomimetic Receptors in Biosensing have been discussed in this review article.

show abstract

Section: Innovations In Biosensing Technologiesmentioning

confidence: 99%

Review—Aptamers and Biomimetic Receptors in Biosensing: Innovations and Applications

Tripathi,

Pandey,

Mishra

et al. 2024

J. Electrochem. Soc.

View full text Add to dashboard Cite

show abstract

“…The first step is the incubation of the target molecule with the library. In this step, the target is incubated into a large, random pool of about 10 15 single-stranded nucleic acid sequences where there is an interaction between the target and nucleic acids [41]. The next step is the segregation of the nucleic acid-target complexes from the unbound sequences and the discarding of the unbound sequences [42].…”

Section: Aptamersmentioning

confidence: 99%

Section: Overview Of Bioaffinity Nanoprobesmentioning

confidence: 99%

Bioaffinity Nanoprobes for Foodborne Pathogen Sensing

Bruce-Tagoe,

Danquah

2023

Micromachines

View full text Add to dashboard Cite

Bioaffinity nanoprobes are a type of biosensor that utilize the specific binding properties of biological molecules, such as antibodies, enzymes, and nucleic acids, for the detection of foodborne pathogens. These probes serve as nanosensors and can provide highly specific and sensitive detection of pathogens in food samples, making them an attractive option for food safety testing. The advantages of bioaffinity nanoprobes include their ability to detect low levels of pathogens, rapid analysis time, and cost-effectiveness. However, limitations include the need for specialized equipment and the potential for cross-reactivity with other biological molecules. Current research efforts focus on optimizing the performance of bioaffinity probes and expanding their application in the food industry. This article discusses relevant analytical methods, such as surface plasmon resonance (SPR) analysis, Fluorescence Resonance Energy Transfer (FRET) measurements, circular dichroism, and flow cytometry, that are used to evaluate the efficacy of bioaffinity nanoprobes. Additionally, it discusses advances in the development and application of biosensors in monitoring foodborne pathogens.

show abstract

“…Since its initial application in 1990, SELEX technology has evolved. Significant advances have been achieved in the design, construction, and use of aptamers, such as making the screening process more efficient, less costly, and less time-consuming [ 35 , 36 , 37 ]. SELEX is an iterative process in which the screening procedure includes the binding of the aptamers, the isolation of target-bound molecules, and subsequent PCR amplification.…”

Section: Introductionmentioning

confidence: 99%

Enriched Aptamer Libraries in Fluorescence-Based Assays for Rikenella microfusus-Specific Gut Microbiome Analyses

Zhang,

Xing,

Bolotnikov

et al. 2023

Microorganisms

View full text Add to dashboard Cite

Rikenella microfusus is an essential intestinal probiotic with great potential. The latest research shows that imbalance in the intestinal flora are related to the occurrence of various diseases, such as intestinal diseases, immune diseases, and metabolic diseases. Rikenella may be a target or biomarker for some diseases, providing a new possibility for preventing and treating these diseases by monitoring and optimizing the abundance of Rikenella in the intestine. However, the current monitoring methods have disadvantages, such as long detection times, complicated operations, and high costs, which seriously limit the possibility of clinical application of microbiome-based treatment options. Therefore, the intention of this study was to evolve an enriched aptamer library to be used for specific labeling of R. microfusus, allowing rapid and low-cost detection methods and, ultimately the construction of aptamer-based biosensors. In this study, we used Rikenella as the target bacterium for an in vitro whole Cell-SELEX (Systematic Evolution of Ligands by EXponential Enrichment) to evolve and enrich specific DNA oligonucleotide aptamers. Five other prominent anaerobic gut bacteria were included in this process for counterselection and served as control cells. The aptamer library R.m-R13 was evolved with high specificity and strong affinity (Kd = 9.597 nM after 13 rounds of selection). With this enriched aptamer library, R. microfusus could efficiently be discriminated from the control bacteria in complex mixtures using different analysis techniques, including fluorescence microscopy or fluorometric suspension assays, and even in human stool samples. These preliminary results open new avenues toward the development of aptamer-based microbiome bio-sensing applications for fast and reliable monitoring of R. microfusus.

show abstract

Advances in Aptamer-Based Biosensors and Cell-Internalizing SELEX Technology for Diagnostic and Therapeutic Application

Cited by 18 publications

References 102 publications

Review—Aptamers and Biomimetic Receptors in Biosensing: Innovations and Applications

Review—Aptamers and Biomimetic Receptors in Biosensing: Innovations and Applications

Bioaffinity Nanoprobes for Foodborne Pathogen Sensing

Enriched Aptamer Libraries in Fluorescence-Based Assays for Rikenella microfusus-Specific Gut Microbiome Analyses

Contact Info

Product

Resources

About