Advances in 3D Vascularized Tumor-on-a-Chip Technology

Jung, Soon-Mo; Jo, Hyeonsu; Hyung, Sujin; Jeon, Noo Li

doi:10.1007/978-3-031-04039-9_9

Cited by 6 publications

(4 citation statements)

References 131 publications

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“…[64] There are numerous MPS that can serve as alternative experimental systems for investigating tumor vascularization. [65] More generally, MPS have been used to model growth factor gradient and interstitial flow field regulation of sprouting angiogenesis. [66,48,67] The MFOC platform used for modeling TNBC angiogenesis is amenable to the formation of an anastomosed and perfusable hierarchical vasculature as we and others have previously reported.…”

Section: Human Microphysiological Systems For Modeling Phytochemical ...mentioning

confidence: 99%

Xenohormetic Phytochemicals Inhibit Neovascularization in Microphysiological Models of Vasculogenesis and Tumor Angiogenesis

Kpeli,

Conrad,

Bralower

et al. 2024

Advanced Biology

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Xenohormesis proposes that phytochemicals produced to combat stressors in the host plant exert biochemical effects in animal cells lacking cognate receptors. Xenohormetic phytochemicals such as flavonoids and phytoalexins modulate a range of human cell signaling mechanisms but functional correlations with human pathophysiology are lacking. Here, potent inhibitory effects of grapefruit‐derived Naringenin (Nar) and soybean‐derived Glyceollins (Gly) in human microphysiological models of bulk tissue vasculogenesis and tumor angiogenesis are reported. Despite this interference of vascular morphogenesis, Nar and Gly are not cytotoxic to endothelial cells and do not prevent cell cycle entry. The anti‐vasculogenic effects of Glyceollin are significantly more potent in sex‐matched female (XX) models. Nar and Gly do not decrease viability or expression of proangiogenic genes in triple negative breast cancer (TNBC) cell spheroids, suggesting that inhibition of sprouting angiogenesis by Nar and Gly in a MPS model of the (TNBC) microenvironment are mediated via direct effects in endothelial cells. The study supports further research of Naringenin and Glyceollin as health‐promoting agents with special attention to mechanisms of action in vascular endothelial cells and the role of biological sex, which can improve the understanding of dietary nutrition and the pharmacology of phytochemical preparations.

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Section: Human Microphysiological Systems For Modeling Phytochemical ...mentioning

confidence: 99%

Xenohormetic Phytochemicals Inhibit Neovascularization in Microphysiological Models of Vasculogenesis and Tumor Angiogenesis

Kpeli,

Conrad,

Bralower

et al. 2024

Advanced Biology

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“…1,2 For instance, 3D tumor models, cell-interaction models, and organ-on-chips have been successfully constructed using microfluidics for elucidating biological mechanisms and drug discovery. [3][4][5] The conventional polydimethylsiloxane (PDMS) chip is usually irreversibly bonded to the substrate and therefore cannot be "opened" unless the device is destroyed. 6 This makes such devices suitable for long-term cell culture, but poses difficulties for efficient extraction of a substantial number of viable cells from the device for downstream manipulation, even with the aid of trypsin.…”

Section: Introductionmentioning

confidence: 99%

Reversibly-bonded microfluidic devices for stable cell culture and rapid, gentle cell extraction

Feng,

Wu,

Cheng

et al. 2024

Lab Chip

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“…[1,2] For instance, 3D tumor models, cell-interaction models, and organ on-chips have been successfully constructed using microfluidics for elucidating biological mechanisms and for drug discovery. [3][4][5] The conventional polydimethylsiloxane (PDMS) chip is usually irreversibly bonded to the substrate and therefore cannot be "opened" unless the device is destroyed. [6] This makes such devices suitable for long-term cell culture, but efficiently extracting a substantial number of viable cells from the device for downstream manipulation is challenging, even with the aid of Trypsin.…”

Section: Introductionmentioning

confidence: 99%

Reversibly-bonded Microfluidic Devices for Stable Cell Culture and Rapid, Gentle Cell Extraction

Feng,

Wai Cheng,

Lin

et al. 2023

Preprint

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Microfluidics chips have emerged as significant tools in cell culture due to their capacity for supporting cells to adopt more physiologically relevant morphology in 3D compared with traditional cell culture in 2D. Currently, irreversible bonding methods commonly used in chip fabrication mean that chips cannot be detached from their substrate without destroying the chip structure, which makes it challenging to do further analysis on cells that have been cultured on-chip. Some reversible bonding techniques exist but are restricted to certain materials, or require complex processing procedures. Here, we demonstrate a simple and reversible polydimethylsiloxane (PDMS)-polystyrene (PS) bonding technique that allows devices to withstand extended operation while pressurized, and supports long-term stable cell cultures. Importantly, it allows rapid and gentle live cell extraction for further downstream manipulation and characterization after long-term on-chip culturing, or even further subculturing. Our new approach could greatly facilitate microfluidic chip-based tissue and cell cultures, overcoming current analytical limitations and opening up new avenues for downstream uses of on-chip cultures, including 3D-engineered tissue structures for biomedical applications.

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Advances in 3D Vascularized Tumor-on-a-Chip Technology

Cited by 6 publications

References 131 publications

Xenohormetic Phytochemicals Inhibit Neovascularization in Microphysiological Models of Vasculogenesis and Tumor Angiogenesis

Xenohormetic Phytochemicals Inhibit Neovascularization in Microphysiological Models of Vasculogenesis and Tumor Angiogenesis

Reversibly-bonded microfluidic devices for stable cell culture and rapid, gentle cell extraction

Reversibly-bonded Microfluidic Devices for Stable Cell Culture and Rapid, Gentle Cell Extraction

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