Advances and prospects of platelet-rich plasma therapy in veterinary ophthalmology

Sharun, Khan; Chandran, Deepak; Manjusha, K.M.; Mankuzhy, Pratheesh; Kumar, Rohit; Pawde, A. M.; Dhama, Kuldeep; El-Husseiny, Hussein M.; Amarpal,

doi:10.1007/s11259-022-10064-z

Cited by 5 publications

(1 citation statement)

References 93 publications

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“…Recent breakthroughs in biomedicine and tissue regeneration have significantly broadened the scope of healthcare [ 1 , 2 , 3 ]. Over the years, a wide range of therapeutic modalities and materials have been employed [ 4 , 5 , 6 , 7 , 8 ]. Mesenchymal stem cells (MSCs) are among the highly investigated cell sources for regenerative medicine and tissue engineering applications [ 9 , 10 ].…”

Section: Introductionmentioning

confidence: 99%

Impact of Adipose Tissue Depot Harvesting Site on the Multilineage Induction Capacity of Male Rat Adipose-Derived Mesenchymal Stem Cells: An In Vitro Study

El-Husseiny

Kaneda

Mady

et al. 2023

IJMS

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Recently, substantial attention has been paid toward adipose-derived mesenchymal stem cells (AdMSCs) as a potential therapy in tissue engineering and regenerative medicine applications. Rat AdMSCs (r-AdMSCs) are frequently utilized. However, the influence of the adipose depot site on the multilineage differentiation potential of the r-AdMSCs is still ambiguous. Hence, the main objective of this study was to explore the influence of the adipose tissue harvesting location on the ability of r-AdMSCs to express the stem-cell-related markers and pluripotency genes, as well as their differentiation capacity, for the first time. Herein, we have isolated r-AdMSCs from the inguinal, epididymal, peri-renal, and back subcutaneous fats. Cells were compared in terms of their phenotype, immunophenotype, and expression of pluripotency genes using RT-PCR. Additionally, we investigated their potential for multilineage (adipogenic, osteogenic, and chondrogenic) induction using special stains confirmed by the expression of the related genes using RT-qPCR. All cells could positively express stem cell marker CD 90 and CD 105 with no significant in-between differences. However, they did not express the hematopoietic markers as CD 34 and CD 45. All cells could be induced successfully. However, epididymal and inguinal cells presented the highest capacity for adipogenic and osteogenic differentiation (21.36-fold and 11.63-fold for OPN, 29.69-fold and 26.68-fold for BMP2, and 37.67-fold and 22.35-fold for BSP, respectively, in epididymal and inguinal cells (p < 0.0001)). On the contrary, the subcutaneous cells exhibited a superior potential for chondrogenesis over the other sites (8.9-fold for CHM1 and 5.93-fold for ACAN, (p < 0.0001)). In conclusion, the adipose tissue harvesting site could influence the differentiation capacity of the isolated AdMSCs. To enhance the results of their employment in various regenerative cell-based therapies, it is thus vital to take the collection site selection into consideration.

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Section: Introductionmentioning

confidence: 99%

Impact of Adipose Tissue Depot Harvesting Site on the Multilineage Induction Capacity of Male Rat Adipose-Derived Mesenchymal Stem Cells: An In Vitro Study

El-Husseiny

Kaneda

Mady

et al. 2023

IJMS

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Smart/stimuli-responsive chitosan/gelatin and other polymeric macromolecules natural hydrogels vs. synthetic hydrogels systems for brain tissue engineering: A state-of-the-art review

El-Husseiny,

Mady,

Doghish

et al. 2024

International Journal of Biological Macromolecules

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Prospects of Platelet-Rich Plasma in Regenerative Medicine

S¹,

Sharun²

2023

OBM Transplant

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Platelet-rich plasma (PRP) is a promising tool in regenerative medicine because it stimulates tissue repair and regeneration. PRP contains a high concentration of platelets, growth factors, and cytokines involved in the healing process. This editorial paper aims to give an overview of PRP in regenerative medicine and its potential applications in various medical fields such as orthopedics, dentistry, dermatology, and wound healing. PRP can be integrated with other regenerative therapies, such as stem cell and gene therapy, to enhance tissue repair and regeneration. Therefore, PRP holds great promise as a regenerative medicine tool, and further research is needed to optimize its use and develop standardized protocols for its preparation and application.

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Advances and prospects of platelet-rich plasma therapy in veterinary ophthalmology

Cited by 5 publications

References 93 publications

Impact of Adipose Tissue Depot Harvesting Site on the Multilineage Induction Capacity of Male Rat Adipose-Derived Mesenchymal Stem Cells: An In Vitro Study

Impact of Adipose Tissue Depot Harvesting Site on the Multilineage Induction Capacity of Male Rat Adipose-Derived Mesenchymal Stem Cells: An In Vitro Study

Smart/stimuli-responsive chitosan/gelatin and other polymeric macromolecules natural hydrogels vs. synthetic hydrogels systems for brain tissue engineering: A state-of-the-art review

Prospects of Platelet-Rich Plasma in Regenerative Medicine

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