Advances and constraints in somatic embryogenesis of Araucaria angustifolia, Acca sellowiana, and Bactris gasipaes

Stefenon, Valdir Marcos; Ree, Joseph Francis; Pinheiro, Marcos Vinícius Marques; Goeten, Daniela; Steiner, Neusa; Guerra, Miguel Pedro

doi:10.1007/s11240-020-01928-w

Cited by 11 publications

(4 citation statements)

References 85 publications

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“…Their fruits are typically consumed fresh, or manufactured as pulp, juice, ice cream, soft drinks, jellies, liqueurs, and wines (Bezerra et al 2004, de Almeida et al 2012). Due to its high adaptability to grow under full sunlight or complete shade, E. uni ora is suitable for the reforestation of degraded areas (Stefenon et al 2020b) and is also important for cosmetics companies due to the presence of essential oils in the leaves. This species also produces secondary metabolites that have been used in traditional medicine to treat gastrointestinal disorders (Costella et al 2013;Teixeira et al 2016) and has potential use in the pharmaceutical industry with antiparasitic, anti-rheumatic, anti-in ammatory, and anti-kinetoplastid activities ( Despite the clear suitability for economic and ecological uses, E. uni ora has been cultivated mainly for domestic purposes, without commercial aspirations (da Silva et al 2014).…”

Section: )mentioning

confidence: 99%

A four steps protocol for in vitro propagation of Eugenia uniflora L. (Myrtaceae)

Franco,

Hermann,

Fritsche

et al. 2023

Genet Resour Crop Evol

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The maintenance of forest formations under a scenario of climatic changes and unsustainable anthropic use of natural resources is vital for the future of mankind. In this context, there is a rising agreement that underutilized crops may be important for the future production of food, as they tend to be more adapted to stressful conditions than domesticated crops. Eugenia uni ora L. (Myrtaceae) is a key species in different forest formations with ecological, economical, and medicinal importance. The present work aimed at establishing an in vitro culture procedure for the mass micropropagation of E. uni ora genotypes through the organogenesis route. Nodal segments from in vitro germinated seeds were introduced in a semi-solid medium for organogenesis establishment and posteriorly cultivated in immersion systems for shoot development and rooting. Different culture media, plant growth regulator concentrations, and immersion systems were tested. The protocol provided in this study may be helpful for the mass production of seedlings for degraded areas recovery or commercial orchard implementation, as well as in genetic improvement programs for E. uni ora.

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Section: )mentioning

confidence: 99%

A four steps protocol for in vitro propagation of Eugenia uniflora L. (Myrtaceae)

Franco,

Hermann,

Fritsche

et al. 2023

Genet Resour Crop Evol

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“…This will help this palm species to not become extinct from natural habitats and meanwhile this technique can be used to preserve germplasm in vitro. In vitro propagation of palm trees through somatic embryogenesis has been achieved (Gomes et al, 2017;Mazri et al, 2017;Heedchim et al, 2020Heedchim et al, , 2021Stefenon et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Development of somatic embryogenesis of mangrove fan palm (Licuala spinosa Thunb.) from culturing immature zygotic embryo

Heedchim

Niha

Yenchon

et al. 2023

Plant Genet. Resour.

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Mangrove fan palm is an endemic plant that grows well and has for a long period been related to traditional ceremonies in southern Thailand. It is an endangered plant with a slow growth and is difficult to propagate in mass production. Tissue culture technique can be used to produce a large number of plants within a short time and to preserve in vitro germplasm resulting in the plant not becoming extinct from natural habitats. Thus, the objectives of this research were to study effects of auxins and embryo preparation on somatic embryogenesis. The sterile immature zygotic embryo was cultured on MS medium with different types and concentrations of auxins. The results showed that 1 mg/l dicamba gave the best results in callus induction at 100% and callus diameter at 4.67 mm after 8 weeks of culture. For callus proliferation, callus chopped at 100 times and transferred to 0.1 mg/l dicamba containing oil palm culture medium gave the highest callus diameter at 10.33 mm after 4 weeks of culture and somatic embryos were also produced. Upon transferring somatic embryos to MS medium with 0.2 M sorbitol, a large number of various developmental stages of secondary somatic embryos were induced within 4 weeks. Therefore, the finding of this study could be highlighted that dicamba and chopping callus have an important role to induce callus and somatic embryo. This is the first report achieved on in vitro propagation of this plant and it will be beneficial for genetic conservation and biotechnological applications in the future.

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“…Therefore, vegetative propagation has been considered for establishing commercial plantations with selected genotypes. However, the use of offshoots (tillers) has given only partially positive results and at relatively low rates ( Flores et al, 2012 ), while micropropagation has been considered a possibility worth further exploration ( Stefenon et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

“…Somatic embryogenesis has been described as the most efficient pathway to obtaining plants in this species. While different explants have been tested, e.g., shoot tips, adventitious buds, leaf primordia, zygotic embryos, inflorescences, and transverse thin cell layers (TCLs) (explants consisting of a few cell layers, usually 0.5–1.0 mm thick, TCLs) (reviewed by Stefenon et al, 2020 ), it is with the latter that most success and highest reproducibility have been obtained. Effective induction of somatic embryogenesis by using TCL has been reported in many species.…”

Section: Introductionmentioning

confidence: 99%

Somatic embryogenesis and plant regeneration from transverse thin cell layers of adult peach palm (Bactris gasipaes) lateral offshoots

et al. 2022

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In this work, we report a successful protocol to obtain in vitro peach palm (Bactris gasipaes Kunth) “Diamantes 10” plants through somatic embryogenesis from transverse thin cell layer (TCL) explants, dissected from three sections (basal, medial, and apical) of lateral offshoots of adult plants cultured on different concentrations of 4-amino-3,5,6-trichloropicolonic acid (picloram). After swelling and development of primary callus in all treatments, without any strong effect of explant origin or picloram concentration, it was possible to observe the formation of embryogenic structures and the exact point from where they developed. Browning was also observed and correlated to the induction treatments, although it was not an impairment for the production of embryogenic structures. Subsequent maturation and conversion of somatic embryos into plantlets allowed their acclimatization 17 months after culture initiation (ACI), which was quicker than previous reports with juvenile tissues (from embryos or seed-germinated plantlets). To the best of our knowledge, this is the first report on peach palm regeneration through somatic embryogenesis from TCL explants from adult plants and could constitute, after fine-tuning the acclimatization stage, a tool for mass clonal propagation of elite genotypes of this open-pollinated crop, as well as for the establishment of conservation strategies of in situ gene bank plant accessions endangered due to aging and other threats.

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Advances and constraints in somatic embryogenesis of Araucaria angustifolia, Acca sellowiana, and Bactris gasipaes

Cited by 11 publications

References 85 publications

A four steps protocol for in vitro propagation of Eugenia uniflora L. (Myrtaceae)

A four steps protocol for in vitro propagation of Eugenia uniflora L. (Myrtaceae)

Development of somatic embryogenesis of mangrove fan palm (Licuala spinosa Thunb.) from culturing immature zygotic embryo

Somatic embryogenesis and plant regeneration from transverse thin cell layers of adult peach palm (Bactris gasipaes) lateral offshoots

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