Advancements made in reproductive research for human uterine transplants

Ramirez, E.R.; Ramírez, Hugo; Ramírez, Diana; Nessetti, M.B.R.; Pillari, V.T.; Khatamee, M.

doi:10.1016/j.fertnstert.2008.07.924

Cited by 1 publication

(2 citation statements)

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“…The group of Brannstron in Gothenburg has already achieved pregnancies in mice uterus transplants (Racho El‐Akouri et al. 2003) and sheep (Brannstrom and Wranning 2007) and Ramirez in goats (Ramirez et al. 2007).…”

Section: Discussionmentioning

confidence: 99%

“…In 2000, a human uterine transplantation was carried out to treat infertility in a woman who had lost her uterus due to post-partum haemorrhage (Fageeh et al 2002). The group of Brannstron in Gothenburg has already achieved pregnancies in mice uterus transplants (Racho El-Akouri et al 2003) and sheep (Brannstrom and Wranning 2007) and Ramirez in goats (Ramirez et al 2007). Xenotransplantation of any organ may, however, be associated with an increased risk of pregnancy complications (McKay and Josephson 2006).…”

Section: Discussionmentioning

confidence: 99%

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Uterus Cryopreservation: Maintenance of Uterine Contractility by the Use of Different Cryoprotocols

Dittrich

Beckmann

Mueller

et al. 2010

Reprod Domestic Animals

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Cryopreservation of cells and even tissue is feasible. New exciting findings arise in the promising field of cryobiology, e.g. the cryopreservation of whole ovaries. Uterus cryopreservation would be advantageous not only for experimental biology, but also for transplantation surgery. The objective of this study was to evaluate various cryopreservation protocols as well as various storage temperatures in cryopreservation of whole swine uteri. The used freezing protocol was slow (0.2 degrees C/min) after arterial perfusion with 1%, 5% or 10% dimethyl sulfoxide (DMSO) solution for 10 min and equilibration in this solution for 30 min. Viability of the organs was tested by histological examination, biochemical parameters and by the capability of rhythmical contractions in a perfusion system. Eighty swine uteri were cryopreserved. All uteri that were frozen with 10% and 5% DMSO were viable after thawing for at least 1 h, whereas only 40% survived with the use of 1% DMSO and 0% with the use of 0.5% DMSO, respectively. There was no difference regarding the survival rates after various cryostorage periods for up to 16 weeks or after cryostorage for 2 weeks in -70 degrees C or -130 degrees C. The cryopreservation of a whole organ such as the swine uterus is a valuable method for the study of cryoprotective agents and freezing protocols. This study demonstrates clearly that the perfusion of the organ with cryoprotectants is the only factor which allows the uterus to contract.

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Section: Discussionmentioning

confidence: 99%