Advancements in fluorescence lifetime imaging microscopy Instrumentation: Towards high speed and 3D

Park, Jongchan; Gao, Liang

doi:10.1016/j.cossms.2024.101147

Cited by 1 publication

(1 citation statement)

References 74 publications

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“…Future directions could explore strategies to minimize fluorescence quenching and toxicity effects, aiming to improve the overall reliability of fluorescence measurements. Additionally, transitioning to 3D-FLIM imaging could offer deeper insights into vesicle dynamics within complex membrane environments [18,63]. However, it also brings challenges, such as longer acquisition time, photon budget considerations and the complexity of data processing.…”

Section: Interaction Of Fluorescently Labelled Luvs and Guvs Of Varyi...mentioning

confidence: 99%

Benefits of Combined Fluorescence Lifetime Imaging Microscopy and Fluorescence Correlation Spectroscopy for Biomedical Studies Demonstrated by Using a Liposome Model System

Bruun,

Löhmannsröben,

Hille

2024

Biophysica

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Drug delivery systems play a pivotal role in targeted pharmaceutical transport and controlled release at specific sites. Liposomes, commonly used as drug carriers, constitute a fundamental part of these systems. Moreover, the drug–liposome model serves as a robust platform for investigating interaction processes at both cellular and molecular levels. To advance our understanding of drug carrier uptake mechanisms, we employed fluorescence lifetime imaging microscopy (FLIM) and fluorescence correlation spectroscopy (FCS), leveraging the unique benefits of two-photon (2P) excitation. Our approach utilized giant unilamellar vesicles (GUVs) as a simplified model system for cell membranes, labelled with the amphiphilic fluorescent dye 3,3′-dioctadecyloxa-carbocyanine (DiOC18(3)). Additionally, large unilamellar vesicles (LUVs) functioned as a drug carrier system, incorporating the spectrally distinct fluorescent sulforhodamine 101 (SRh101) as a surrogate drug. The investigation emphasized the diverse interactions between GUVs and LUVs based on the charged lipids employed. We examined the exchange kinetics and structural alterations of liposome carriers during the uptake process. Our study underscores the significance of employing 2P excitation in conjunction with FLIM and FCS. This powerful combination offers a valuable methodological approach for studying liposome interactions, positioning them as an exceptionally versatile model system with a distinct technical advantage.

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Section: Interaction Of Fluorescently Labelled Luvs and Guvs Of Varyi...mentioning

confidence: 99%

Benefits of Combined Fluorescence Lifetime Imaging Microscopy and Fluorescence Correlation Spectroscopy for Biomedical Studies Demonstrated by Using a Liposome Model System

Bruun,

Löhmannsröben,

Hille

2024

Biophysica

View full text Add to dashboard Cite

show abstract

Advancements in fluorescence lifetime imaging microscopy Instrumentation: Towards high speed and 3D

Cited by 1 publication

References 74 publications

Benefits of Combined Fluorescence Lifetime Imaging Microscopy and Fluorescence Correlation Spectroscopy for Biomedical Studies Demonstrated by Using a Liposome Model System

Benefits of Combined Fluorescence Lifetime Imaging Microscopy and Fluorescence Correlation Spectroscopy for Biomedical Studies Demonstrated by Using a Liposome Model System

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