Advancement of milk protein analysis: From determination of total proteins to their identification and quantification by proteomic approaches

Warakaulle, Santhoshani; Mohamed, Huda; Ranasinghe, Meththa; Shah, Iltaf; Yanyang, Xu; Chen, Gang; Ayyash, Mutamed M.; Vincent, Delphine; Kamal-Eldin, Afaf

doi:10.1016/j.jfca.2023.105854

Cited by 3 publications

(1 citation statement)

References 182 publications

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“…Due to the complexity of the matrix of milk and dairy products and the demands of legislation with respect to their manufacture, storage, and marketing, all categories of analytical methods are applied-often in combinations-in dairy research, product development, and quality control (e.g., [40][41][42][43][44]). The study of Zobkova, Yurova, Semipyatniy, et al proposes an innovative application of a biotesting procedure based on the assessment of protozoa metabolism.…”

Section: Emerging Technologies In the Dairy Sector: Some Research Exa...mentioning

confidence: 99%

Emerging Technologies for Improving Properties, Shelf Life, and Analysis of Dairy Products

Moatsou

2024

Foods

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Section: Emerging Technologies In the Dairy Sector: Some Research Exa...mentioning

confidence: 99%

Emerging Technologies for Improving Properties, Shelf Life, and Analysis of Dairy Products

Moatsou

2024

Foods

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Phosphopeptides of casein: potential nutraceuticals

Li,

Zhang,

Xie

et al. 2024

Casein

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Changes in Protein Levels during the Storage and Warming of Breast Milk in a Domestic Environment

Siviroj,

Ruangsuriya,

Ongprasert

2024

Children

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Background/Objectives: Storage and warming can impact the protein concentration of breast milk (BM). This study aimed to explore the effects of routine BM handling, from storage to warming, on BM protein concentration. Methods: Forty BM samples were collected from 40 mothers with full-term infants. Fresh BM samples were analyzed within 24 h of expression as a baseline. Additional samples were stored in a refrigerator for up to 4 days and in either a freezer compartment of a refrigerator with a separate door (refrigerator freezers) or a deep freezer for up to 6 months. We compared four warming techniques: rapid thawing in water at 25 °C and 37 °C immediately after freezer removal, and slow thawing in a refrigerator for 12 h, followed by warming in water at 25 °C and 37 °C. The protein concentration was measured using the Lowry method. Results: No significant decrease in BM protein concentration was observed across all storage conditions compared to baseline. BM with a higher protein content benefited more from storage in deep freezers than in refrigerator freezers. Thawing immediately after removal from the freezer at 25 °C preserved significantly higher total protein levels compared to alternative heating techniques. Conclusions: Our findings support the recommendation for mothers to store BM in either type of freezer for up to 6 months. Our results suggest that rapid thawing at 25 °C is the most effective method for preserving protein levels compared to other thawing techniques used in our study.

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Advancement of milk protein analysis: From determination of total proteins to their identification and quantification by proteomic approaches

Cited by 3 publications

References 182 publications

Emerging Technologies for Improving Properties, Shelf Life, and Analysis of Dairy Products

Emerging Technologies for Improving Properties, Shelf Life, and Analysis of Dairy Products

Phosphopeptides of casein: potential nutraceuticals

Changes in Protein Levels during the Storage and Warming of Breast Milk in a Domestic Environment

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