Advancement of Metabolic Engineering Assisted by Synthetic Biology

Lee, Hyang-Mi; Vo, Phuong; Na, Dokyun

doi:10.3390/catal8120619

Cited by 19 publications

(11 citation statements)

References 124 publications

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“…It can even be used to obtain flavoured compounds that allow the product to be labelled as a "natural product". The use of solid-state fermentation can provide higher incomes or more suitable product characteristics than submerged fermentation with inferior economic values [78,79].…”

Section: Functional Characterisation and Metabolic Engineering Of Flamentioning

confidence: 99%

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Beverage and Food Fragrance Biotechnology, Novel Applications, Sensory and Sensor Techniques: An Overview

Vilela

Bacelar

Pinto

et al. 2019

Foods

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Flavours and fragrances are especially important for the beverage and food industries. Biosynthesis or extraction are the two main ways to obtain these important compounds that have many different chemical structures. Consequently, the search for new compounds is challenging for academic and industrial investigation. This overview aims to present the current state of art of beverage fragrance biotechnology, including recent advances in sensory and sensor methodologies and statistical techniques for data analysis. An overview of all the recent findings in beverage and food fragrance biotechnology, including those obtained from natural sources by extraction processes (natural plants as an important source of flavours) or using enzymatic precursor (hydrolytic enzymes), and those obtained by de novo synthesis (microorganisms’ respiration/fermentation of simple substrates such as glucose and sucrose), are reviewed. Recent advances have been made in what concerns “beverage fragrances construction” as also in their application products. Moreover, novel sensory and sensor methodologies, primarily used for fragrances quality evaluation, have been developed, as have statistical techniques for sensory and sensors data treatments, allowing a rapid and objective analysis.

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Section: Functional Characterisation and Metabolic Engineering Of Flamentioning

confidence: 99%

“…incomes or more suitable product characteristics than submerged fermentation with inferior economic values [78,79]. Figure 1.…”

Section: Functional Characterisation and Metabolic Engineering Of Flamentioning

confidence: 99%

Beverage and Food Fragrance Biotechnology, Novel Applications, Sensory and Sensor Techniques: An Overview

Vilela

Bacelar

Pinto

et al. 2019

Foods

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“…Processes 2020, 8, 502 2 of 13 A Gram-positive, spore-forming obligate aerobe, A. acidocaldarius has been shown to grow on a variety of carbon sources, including glucose, fructose, mannose, ribose, arabinose, xylose, galactose, rhamnose, glycerol, mannitol, methylglucoside, arbutin, aesculin, salicin, cellobiose, maltose, lactose, melibiose, sucrose, trehalose, raffinose, starch, and glycogen [11]. This organism has the ability to degrade hemicellulose and cellulose [12,13], making it very useful for biomass processing applications. In particular, energy-limited chemostat growth on glucose has been characterized under different temperatures and pH [14,15].…”

Section: Introductionmentioning

confidence: 99%

Metabolic Efficiency of Sugar Co-Metabolism and Phenol Degradation in Alicyclobacillus acidocaldarius for Improved Lignocellulose Processing

Beck

2020

Processes

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Substrate availability plays a key role in dictating metabolic strategies. Most microorganisms consume carbon/energy sources in a sequential, preferential order. The presented study investigates metabolic strategies of Alicyclobacillus acidocaldarius, a thermoacidophilic bacterium that has been shown to co-utilize glucose and xylose, as well as degrade phenolic compounds. An existing metabolic model was expanded to include phenol degradation and was analyzed with both metabolic pathway and constraint-based analysis methods. Elementary flux mode analysis was used in conjunction with resource allocation theory to investigate ecologically optimal metabolic pathways for different carbon substrate combinations. Additionally, a dynamic version of flux balance analysis was used to generate time-resolved simulations of growth on phenol and xylose. Results showed that availability of xylose along with glucose did not predict enhanced growth efficiency beyond that of glucose alone, but did predict some differences in pathway utilization and byproduct profiles. In contrast, addition of phenol as a co-substrate with xylose predicted lower growth efficiency. Dynamic simulations predicted co-consumption of xylose and phenol in a similar pattern as previously reported experiments. Altogether, this work serves as a case study for combining both elementary flux mode and flux balance analyses to probe unique metabolic features, and also demonstrates the versatility of A. acidocaldarius for lignocellulosic biomass processing applications.

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“…From combinatorial optimization to efficient production Synthetic biology tools 41 and design principles (Fig. 2, black arrow) are being used to accelerate development of combinatorial optimization methods.…”

mentioning

confidence: 99%

Application of combinatorial optimization strategies in synthetic biology

Naseri¹,

Koffas²

2020

Nat Commun

105

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In the first wave of synthetic biology, genetic elements, combined into simple circuits, are used to control individual cellular functions. In the second wave of synthetic biology, the simple circuits, combined into complex circuits, form systems-level functions. However, efforts to construct complex circuits are often impeded by our limited knowledge of the optimal combination of individual circuits. For example, a fundamental question in most metabolic engineering projects is the optimal level of enzymes for maximizing the output. To address this point, combinatorial optimization approaches have been established, allowing automatic optimization without prior knowledge of the best combination of expression levels of individual genes. This review focuses on current combinatorial optimization methods and emerging technologies facilitating their applications.

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Advancement of Metabolic Engineering Assisted by Synthetic Biology

Cited by 19 publications

References 124 publications

Beverage and Food Fragrance Biotechnology, Novel Applications, Sensory and Sensor Techniques: An Overview

Beverage and Food Fragrance Biotechnology, Novel Applications, Sensory and Sensor Techniques: An Overview

Metabolic Efficiency of Sugar Co-Metabolism and Phenol Degradation in Alicyclobacillus acidocaldarius for Improved Lignocellulose Processing

Application of combinatorial optimization strategies in synthetic biology

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