Advanced Short Tandem Repeat Genotyping for Forensic Human Identification

Kim, Yong T.; Heo, Hyun Young; Seo, Tae Seok

doi:10.1002/9783527803293.ch29

Cited by 2 publications

(2 citation statements)

References 91 publications

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“…Nowadays, short tandem repeats are considered the standard test for quantitative chimerism analysis, 20 genotyping in parentage testing 21 and forensic human identity testing. 22 Many studies compared the precision and efficacy of VNTR in comparison with STR. Mossallam et al 12 concluded that there is concordance in chimerism results when they compared both techniques results together.…”

Section: Discussionmentioning

confidence: 99%

Study of whole blood and T cell chimerism in monitoring HLA identical allogeneic hematopoietic transplantation

Deghady¹,

Elsorady²,

Lewis³

et al. 2018

HTIJ

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Chimerism analysis is an important method for monitoring outcome of allogenic hematopoietic stem cell transplantation. Variable number tandem (VNTR) analysis is considered an informative technique to follow up chimerism state. The aim of the work is to study certain number of VNTRs to identify their potential value in the detection of chimerism in transplanted patients in conventional ablative transplants. Also to demonstrate the value of T cells subset analysis in detecting transplantation outcomes. This study included 17 pairs undergoing HSCT. Informative loci pre-transplantation using five VNTR loci and two gene loci were identified. After transplantation the informative loci were used to detect chimerism status. After DNA extraction from blood samples, amplification of VNTR loci was performed using a conventional PCR protocol. Extra sample post transplantation was collected and pre-treated with resetting technique in order to separate T cells, its product was subjected to DNA extraction then amplification of informative loci was done. Amplified product of DNA samples was run on 2% agarose gel stained using ethidium bromide. Fourteen recipients showed full done chimerism in both whole blood and T cells separated cells, one recipient died after HSCT, one recipient showed split chimerism and one pair failed to detect informative locus. VNTR analysis using a panel of five loci is suitable to detect state of chimerism after HSCT.

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Section: Discussionmentioning

confidence: 99%

Study of whole blood and T cell chimerism in monitoring HLA identical allogeneic hematopoietic transplantation

Deghady¹,

Elsorady²,

Lewis³

et al. 2018

HTIJ

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show abstract

“…Pada umumnya, variasi atau keragaman manusia dapat diteliti berdasarkan penanda molekuler maupun penanda non molekuler. Analisis keragaman manusia berdasarkan penanda molekuler dapat dilakukan dengan menggunakan penanda DNA Mikrosatelit (Kim et al, 2018). Adapun analisis keragaman manusia berdasarkan penanda non molekuler dapat dilakukan melalui proses somatologi, biotipologi dan androgini (Molnar, 2006).…”

Section: Pendahuluanunclassified

Cephalometry Variation of Bataknese

Gaol¹

2022

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Indonesia merupakan negara dengan penduduk yang terdiri dari berbagai macam etnis dan suku bangsa, salah satu suku bangsa terbesar di Indonesia adalah suku Batak. Suku Batak terbagi menjadi beberapa subsuku, dengan subsuku terbesar yaitu Batak Toba, Batak Karo, dan Batak Simalungun. Pada penelitian ini dilakukan analisis variasi kefalometri pada suku Batak. Adanya peningkatan jumlah populasi dari suku bangsa di berbagai wilayah, diikuti dengan meningkatnya aktivitas lalu lintas dan kuantitas tindak kejahatan dapat menyebabkan peningkatan peluang terjadinya kecelakaan lalu lintas dan korban kejahatan. Oleh karena itu, penelitian ini perlu dilakukan untuk mengetahui variasi kefalometri dan indeks kefalometri pada masyarakat suku Batak yang dapat digunakan dalam proses pengungkapan identitas korban kejahatan maupun kecelakaan lalu lintas. Penelitian ini melibatkan 60 subjek suku Batak yang terdiri dari 20 orang subsuku Batak Toba, 20 orang subsuku Batak Karo, dan 20 orang subsuku Batak Simalungun. Pada penelitian ini dilakukan tahapan pengajuan kelayakan etik (ethical clearance), pendataan subjek, pengukuran subjek, perhitungan indeks kefalometri, dan analisis data. Hasil yang diperoleh pada penelitian ini menunjukkan bahwa subsuku Batak Toba, Karo, dan Simalungun memiliki tipe kepala brachycephalic, tipe dahi lebar, dan tipe hidung platyrrhine. Adapun pada subsuku Batak Toba dan Karo diketahui memiliki tipe wajah leptoprosopic sedangkan pada subsuku Batak Simalungun memiliki tipe wajah mesoprosopic. Pada penelitian ini juga diketahui bahwa antara subsuku Batak Toba dengan Batak Karo tidak terdapat perbedaan yang signifikan pada seluruh data ukuran tubuh dan kefalometri, namun antara subsuku Batak Toba dengan Batak Simalungun terdapat perbedaan yang signifikan pada lebar minimum frontal, lebar hidung, dan frontoparietal index, sedangkan antara subsuku Batak Simalungun dengan Batak Karo terdapat perbedaan yang signifikan pada lebar minimum frontal dan lebar wajah.

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Advanced Short Tandem Repeat Genotyping for Forensic Human Identification

Cited by 2 publications

References 91 publications

Study of whole blood and T cell chimerism in monitoring HLA identical allogeneic hematopoietic transplantation

Study of whole blood and T cell chimerism in monitoring HLA identical allogeneic hematopoietic transplantation

Cephalometry Variation of Bataknese

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