Advanced oxidation protein product level in children with chronic otitis media with effusion

Balikci, Hasan Huseyin; Karakaş, Mustafa Serkan; Gurdal, M. Mustafa; Özkul, Murat Haluk; Bayram, Özlem; Bayram, Ali̇; Yigit, Servet

doi:10.1016/j.ijporl.2014.01.007

Cited by 8 publications

(6 citation statements)

References 15 publications

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“…Alternatively, loss of adaptation to ER stress (Guan et al, 2017) can also increase proinflammatory gene expression via activation of NF-kB (Schmitz et al, 2018). Both of these mechanisms can contribute to the inflammatory process in OM (Aydogan et al, 2013;Balikci et al, 2014). This is consistent with previous research, which found that ER stress, inflammation, and oxidative stress are tightly integrated and each one can influence the other (Zhang et al, 2017;Chen et al, 2019;Diaz-Bulnes et al, 2019).…”

Section: Figure 4 |supporting

confidence: 89%

Role of Endoplasmic Reticulum Stress in Otitis Media

Zhao

Wang

et al. 2020

Front. Genet.

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Endoplasmic reticulum (ER) stress occurs in many inflammatory responses. Here, we investigated the role of ER stress and its associated apoptosis in otitis media (OM) to elucidate the mechanisms of OM and the signaling crosstalk between ER stress and other cell damage pathways, including inflammatory cytokines and apoptosis. We examined the expression of inflammatory cytokine-and ER stress-related genes by qRT-PCR, Western blotting, and immunohistochemistry (IHC) in the middle ear of C57BL/6J mice after challenge with peptidoglycan polysaccharide (PGPS), an agent inducing OM. We also evaluated the effect of the suppression of ER stress with tauroursodeoxycholic acid (TUDCA), an ER stress inhibitor. The study revealed the upregulation of ER stressand apoptosis-related gene expression after the PGPS treatment, specifically ATF6, CHOP, BIP, caspase-12, and caspase-3. TUDCA treatment of PGPS-treated mice decreased OM; reduced the expression of CHOP, BIP, and caspase 3; and significantly decreased the proinflammatory gene expression of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6). These results suggest that PGPS triggers ER stress and downstream proinflammatory gene expression in OM and that inhibition of ER stress alleviates OM. We propose that ER stress plays a critical role in inflammation and cell death, leading to the development of OM and points to ER stress inhibition as a potential therapeutic approach for the prevention of OM.

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Section: Figure 4 |supporting

confidence: 89%

Role of Endoplasmic Reticulum Stress in Otitis Media

Zhao

Wang

et al. 2020

Front. Genet.

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“…Balikci et al measured oxidative protein product levels in middle ear fluid and plasma obtained from 25 children with COM with effusion (COME) and 30 healthy control children. 29 While plasma oxidative protein product levels did not differ between COME and control children, MEE fluid oxidative protein levels were significantly higher than plasma oxidative protein levels in the COME group. The role of protein oxidation imbalance in the pathogenesis of COME warrants further investigation.…”

Section: Oxidative Stress In Middle Ear Inflammatory Responsementioning

confidence: 72%

“…They found a significant imbalance in oxidative stress and antioxidant enzyme levels in COM patients with cholesteatoma. Balikci et al measured oxidative protein product levels in middle ear fluid and plasma obtained from 25 children with COM with effusion (COME) and 30 healthy control children 29 . While plasma oxidative protein product levels did not differ between COME and control children, MEE fluid oxidative protein levels were significantly higher than plasma oxidative protein levels in the COME group.…”

Section: Discussionmentioning

confidence: 99%

Panel 8: Report on Recent Advances in Molecular and Cellular Biochemistry

Preciado

Granath

Lin

et al. 2017

Otolaryngol.--head neck surg.

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Objectives. To update the medical literature on recent cellular and molecular advances in otitis media disease models with a principal focus on developments in the past 5 years. We also aim to explain recent translational advances in cellular and molecular biology that have influenced our understanding and management of otitis media.Data Sources. PubMed-indexed peer-reviewed articles.Review Methods. A comprehensive review of the literature was conducted with the term otitis media and the following search terms: molecular biology, cell biology, innate immunity, oxidative stress, mucins, molecular diagnostics. Included articles were published in the English language from January 1, 2010, to July 31, 2015.Implications for Practice. The molecular understanding of otitis media disease progression has rapidly advanced over the last 5 years. The roles of inflammation, mucins, and cell signaling mechanisms have been elucidated and defined. Advances in the field provide a plethora of opportunities for innovative molecular targeting in the development of novel therapeutic strategies for otitis media. I nfections in the middle ear trigger a series of cellular responses in middle ear epithelium. The subsequent pathogenesis of otitis media (OM) from acute to chronic, however, is complex and multifactorial. Mechanisms of propensity to OM have been shown to involve, among many other factors, mutations or polymorphisms in genes that mediate innate immunity and cellular signaling pathways that promote healing and recovery after inflammation. According to recent reports, the following cellular signaling pathways are altered following OM infection: Toll-like receptor (TLR) cascade, the inflammasome, NF-kB signaling, and the TGF-b pathway. During disease progression, mucous cell metaplasia or hyperplasia is frequently observed in animal models and human tissue specimens. In turn, the occurrence of mucous cell metaplasia or hyperplasia promotes the chronicity of OM as well as relapses of acute infections due to the abundant production of mucin glycoproteins. This State of the Art Review updates recent advances in innate immune and cell signaling responses, genetic predispositions that affect molecular pathways, mucin responses, oxidative stress, and novel molecular diagnostics. MethodsA comprehensive review of the medical literature was conducted by the Panel 8 members of the Post-Conference

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“…Balikci et al 18 investigated AOPP levels in children with chronic otitis media with effusion; they found that AOPP levels were increased in the effusion fluid, but not in the plasma, of these patients. The authors, therefore, concluded that chronic otitis media with effusion was associated with protein oxidation abnormalities.…”

Section: Discussionmentioning

confidence: 99%

Acta Otorhinolaryngologica Italica

Özbay

Kucur

Koçak

et al. 2016

Acta Otorhinolaryngol Ital

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Lobiettivo del presente studio è stato determinare se i livelli plasmatici dei prodotti avanzati di ossidazione proteica (AOPP) rappresentino dei marker di stress ossidativo nei pazienti pediatrici affetti da tonsillite cronica. Per lo studio sono stati arruolati, presso i Dipartimenti di Otorinolaringoiatria e Chirurgia pediatrica dellOspedale Universitario di Dumlupinar, trenta bambini sani e trenta affetti da tonsillite cronica. Il gruppo dei pazienti affetti da malattia è stato sottoposto a un prelievo ematico preoperatorio e ad una biopsia intraoperatoria del tessuto tonsillare. Il gruppo dei pazienti sani è stato sottoposto unicamente al prelievo ematico. I livelli plasmatici e tissutali degli AOPP sono quindi stati misurati mediante spettrofotometria. I livelli sierici degli AOPP sono risultati essere più elevati nel gruppo dei pazienti affetti da tonsillite cronica (13,1 ± 3,3 ng/ml) rispetto al gruppo di controllo (11,6 ± 2,3 ng/ml; P < 0,05). Il livello tissutale medio degli AOPP nei pazienti malati è risultato essere superiore a quello plasmatico medio sia nel gruppo dei pazienti sani che in quello dei pazienti malati (41,9 ± 13,5 ng/mg; P < 0,05). I livelli plasmatici e tissutali degli AOPP sono risultati quindi essere più elevati nei pazienti malati rispetto al gruppo di controllo. Gli AOPP potrebbero quindi rappresentare una nuova classe di molecole pro-infiammatorie coinvolte nello stress ossidativo nella tonsillite cronica e potrebbero avere un ruolo come marker di stress ossidativo nei pazienti pediatrici affetti da tale patologia.

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Advanced oxidation protein product level in children with chronic otitis media with effusion

Cited by 8 publications

References 15 publications

Role of Endoplasmic Reticulum Stress in Otitis Media

Role of Endoplasmic Reticulum Stress in Otitis Media

Panel 8: Report on Recent Advances in Molecular and Cellular Biochemistry

Acta Otorhinolaryngologica Italica

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