Advanced oxidation processes applied for color removal of textile effluent using a home-made peroxidase from rice bran

Klanovicz, Natalia; Camargo, Aline Frumi; Stefanski, Fábio Spitza; Zanivan, Jessica; Scapini, Thamarys; Pollon, Rafaela; Warken, Andressa; Paliga, Letícia; Preczeski, Karina Paula; Ribeiro, Anderson André Genro Alves; Garda‐Buffon, Jaqueline; Fongaro, Gislaine; Treichel, Helen

doi:10.1007/s00449-019-02222-6

Cited by 17 publications

(10 citation statements)

References 40 publications

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“…Also, for the blue dye, two peroxidase sources (Ipomea palmata and Saccharum spontaneum) showed removals of 15% and 70%, respectively, after 1 to 2 hours of treatment, indicating different speci cities of plant peroxidase to the same dye (Sha qu et al, 2002). Enzymatic treatments conducted in other reactional systems also provided different color removal results, as shown by a previous study (Klanovicz et al, 2020), in which rice bran peroxidase removed 39% of the color after 3 hours of reaction in a microwave system, while complete removal was achieved after 24 h on an orbital shaker.…”

Section: Relationship Between Enzymatic Activity and Discoloration Potentialsupporting

confidence: 51%

Complete wastewater discoloration by a novel peroxidase source with promising bioxidative properties

Klanovicz

Stefanski

Camargo

et al. 2021

Preprint

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The occurrence of micropollutants in aqueous matrices has become a global concern and a challenge for wastewater treatment plants. Monitoring their toxicity has shown that these compounds, even at low concentrations, pose risks to human and environmental health. Therefore, our study aimed to prospect immobilization strategies for non-commercial oxidoreductase enzymes and insert them in the context of pollutant remediation. The enzymatic extract was obtained by submerged fermentation of the fungus Trichoderma koningiopsis in an alternative substrate, consisting of fresh microalgal biomass from the phycoremediation process. The immobilization efficiency of peroxidase (POD) was evaluated by monitoring the residual activity (RA) and the discoloration potential (DP) of a synthetic dye solution. Concomitantly, the catalytic properties of free POD were explored, and the most promising storage strategy to maintain the enzymatic activity was studied. Guaiacol peroxidase from T. koningiopsis expressed specific activity of up to 7801.1 U mg− 1 in the free form, showing stability when subjected to up 80°C in a pH range between 4.0–8.0. Furthermore, the bioproduct immobilized on magnetic nanoparticles expressed up to 688% RA and 100% DP. An increase in the RA of the enzyme, both in free and immobilized form, was also observe after storage for up to 8 months. The synthesized magnetic nanozymes showed good reusability, maintaining 13546.4 U mg− 1 after ten cycles and removing 93% of color in a second batch. Toxicological evaluation with Allium cepa indicated that the enzymatic process of color removal with immobilized POD, despite maintaining unwanted cytotoxic effects, was essential to eliminate genotoxic effects. In this sense, the immobilization processes of T. koningiopsis peroxidase presented in our work are promising for the enzyme market and for the wastewater treatment sector.

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Section: Relationship Between Enzymatic Activity and Discoloration Potentialsupporting

confidence: 51%

Complete wastewater discoloration by a novel peroxidase source with promising bioxidative properties

Klanovicz

Stefanski

Camargo

et al. 2021

Preprint

Self Cite

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“…Also, for the blue dye, two peroxidase sources (Ipomea palmata and Saccharum spontaneum) showed removals of 15% and 70%, respectively, after 1 to 2 hours of treatment, indicating different speci cities of plant peroxidase to the same dye 30 . Enzymatic treatments conducted in other reactional systems also provided different color removal results, as shown by a previous study 18 , in which rice bran peroxidase removed 39% of the color after 3 hours of reaction in a microwave system, while complete removal was achieved after 24 h in an orbital shaker.…”

Section: Relationship Between Enzymatic Activity and Discoloration Potentialmentioning

confidence: 84%

“…The dyes studied were red (455 nm), blue (595 nm), yellow (414 nm), and brown (530 nm). The tests were conducted according to previous studies 18 . The dye solution, prepared with distilled water at 100 mg L -1 , was treated with 5 or 10 mL of crude enzymatic extract and 40 mg L -1 of hydrogen peroxide 35% v v -1 .…”

Section: Discoloration Studiesmentioning

confidence: 99%

Complete wastewater discoloration by a novel peroxidase source with promising bioxidative properties

Klanovicz

Stefanski

Camargo

et al. 2022

Preprint

Self Cite

View full text Add to dashboard Cite

BACKGROUND Our study aimed to characterize and prospect immobilization strategies for a novel fungal peroxidase - POD (EC 1.11.1.7) and to insert it in the context of pollutant remediation, since these compounds pose risks to human and environmental health. The enzymatic extract was obtained by submerged fermentation of the fungus Trichoderma koningiopsis in an alternative substrate, consisting of fresh microalgal biomass. The immobilization efficiency was evaluated by monitoring the residual activity (RA) and the discoloration potential (DP) of a synthetic dye solution. Concomitantly, the catalytic properties of free POD were explored, and the most promising storage strategy to maintain the enzymatic activity was studied. RESULTS The novel guaiacol peroxidase expressed specific activity of up to 7801 U mg−1 in the free form, showing stability when subjected to up to 80°C in a pH range between 4.0-8.0. Furthermore, the bioproduct immobilized on magnetic nanoparticles expressed up to 689% RA and 100% DP. An increase in the RA of the enzyme, both in free and immobilized form, was also observed after storage for up to 8 months. The synthesized magnetic nanozymes showed good reusability, maintaining 13546 U mg−1 after ten cycles and removing 94% of color in a second batch. Toxicological evaluation with Allium cepa indicated that the enzymatic process of color removal with immobilized POD was essential to eliminate genotoxic effects. CONCLUSION T. koningiopsis peroxidase production and immobilization presented in our work are promising for the enzyme market and for the wastewater treatment technologies, considering its high bioxidative potential.

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“…It is therefore imperative that such colourants be eliminated from regular water bodies and supplies. In this sense, several physico-chemical technologies, such as coagulation/flocculation [ 8 , 9 ], chemical oxidation [ 10 , 11 , 12 , 13 ], ion exchange [ 14 , 15 , 16 ], membrane separation [ 17 , 18 ], electrochemical techniques [ 19 , 20 ], adsorption [ 6 , 21 ] and photo-catalysis [ 22 , 23 , 24 , 25 ], have been explored in the remediation of dye-containing wastewaters. However, drawbacks such as low efficiency, high sludge generation, intensive energy requirements, and most importantly their high installation cost associated with membrane technology [ 18 , 26 ] impede the application of such technologies for the remediation of MO.…”

Section: Introductionmentioning

confidence: 99%

Polyaniline nanofibers, a nanostructured conducting polymer for the remediation of Methyl orange dye from aqueous solutions in fixed-bed column studies

Dlamini¹,

Bhaumik²,

Pillay³

et al. 2021

Heliyon

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Polyaniline nanofibers (PANI NFs) were synthesized and employed as potential adsorbents in a continuous flow fixed-bed column adsorption study for an organic dye, Methyl Orange (MO) removal from water. These nanostructured adsorbents were characterized using ATR-FTIR, FE-SEM, HR-TEM, TGA, BET, XRD, XPS, and the Zetasizer. Morphological representations from SEM and TEM analyses showed that the fibers were nanosized with diameters lower than 80 nm and an interconnected network possessing a smooth surface. The S BET of the PANI NFs was found to be 35.80 m 2 /g. The impact of column design parameters for instance; influent concentration, flow rate, and bed mass was investigated using pH 4 influent MO solutions optimized through batch studies. The best influent concentration, bed length, and flow rate for this study were determined as 25 mg/L, 9 cm (6 g), and 3 mL/min, respectively. The column information was fitted in Thomas, Yoon-Nelson, and Bohart-Adams models. It appeared that the Thomas and Yoon-Nelson models described the data satisfactorily. The PANI NFs were able to treat 29.16 L of 25 mg/L MO solution at 9 cm bed length. A sulfate peak in a de-convoluted sulfur spectrum using XPS verified the successful adsorption of Methyl Orange.

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Advanced oxidation processes applied for color removal of textile effluent using a home-made peroxidase from rice bran

Cited by 17 publications

References 40 publications

Complete wastewater discoloration by a novel peroxidase source with promising bioxidative properties

Complete wastewater discoloration by a novel peroxidase source with promising bioxidative properties

Complete wastewater discoloration by a novel peroxidase source with promising bioxidative properties

Polyaniline nanofibers, a nanostructured conducting polymer for the remediation of Methyl orange dye from aqueous solutions in fixed-bed column studies

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