Advanced Optical Imaging Requiring No Contrast Agents—A New Armamentarium for Medicine and Surgery

Chung, Alice; Wachsmann‐Hogiu, Sebastian; Zhao, Tong; Xiong, Yizhi; Joseph, A.; Farkas, Daniel L.

doi:10.1016/j.cursur.2004.12.011

Cited by 11 publications

(8 citation statements)

References 18 publications

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“…The resulting data can be exploited using the spectral content only, or with novel tools, both spectral and spatial information can be combined in the analytical process, using machine-learning approaches for optimization. The next step is to perform hyperspectral fluorescence imaging for in vivo detection of malignant tissues and cells (Chung et al 2005(Chung et al , 2006Zavattini et al 2006) and a combination of these with additional in vivo imaging modalities (Ntziachristos et al 2005). These technology combinations may further help to unravel processes in tumour development and tumour cell migration in vivo (Wolf et al 2003;Wolf & Friedl 2006).…”

Section: Quality Control and Standardization In Systemic Analysismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Cytomics – importance of multimodal analysis of cell function and proliferation in oncology

2006

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Cancer is a highly complex and heterogeneous disease involving a succession of genetic changes (frequently caused or accompanied by exogenous trauma), and resulting in a molecular phenotype that in turn results in a malignant specification. The development of malignancy has been described as a multistep process involving self-sufficiency in growth signals, insensitivity to antigrowth signals, evasion of apoptosis, limitless replicative potential, sustained angiogenesis, and finally tissue invasion and metastasis. The quantitative analysis of networking molecules within the cells might be applied to understand native-state tissue signalling biology, complex drug actions and dysfunctional signalling in transformed cells, that is, in cancer cells. High-content and high-throughput single-cell analysis can lead to systems biology and cytomics. The application of cytomics in cancer research and diagnostics is very broad, ranging from the better understanding of the tumour cell biology to the identification of residual tumour cells after treatment, to drug discovery. The ultimate goal is to pinpoint in detail these processes on the molecular, cellular and tissue level. A comprehensive knowledge of these will require tissue analysis, which is multiplex and functional; thus, vast amounts of data are being collected from current genomic and proteomic platforms for integration and interpretation as well as for new varieties of updated cytomics technology. This overview will briefly highlight the most important aspects of this continuously developing field.

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Section: Quality Control and Standardization In Systemic Analysismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Cytomics – importance of multimodal analysis of cell function and proliferation in oncology

2006

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“…New horizons can be opened by the new SBC techniques of structural and functional high‐resolution analysis from intracellular and membrane components (confocal microscopy, L1, nanoscopy, L18) (17), total internal fluorescence microscopy (TIRFM, L32) (18), and tissue level (tissomics, P10, L21) (19–22), to organ and organism level (in vivo optical imaging, L22) (23).…”

Section: Progress In Cell Analyticmentioning

confidence: 99%

Systems biology and clinical cytomics: The 10th Leipziger workshop and the 3rd international workshop on slide‐based cytometry

2005

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Despite very significant technical and software improvements in flow cytometry (FCM) since the 1980's, the demand for a cytometric technology combining both quantitative cell analysis and morphological documentation in Cytomics became evident. Improvements in microtechnology and computing permit nowadays similar quantitative and stoichiometric single cell‐based high‐throughput analyses by microscopic instruments, like Slide‐Based Cytometry (SBC). SBC and related techniques offer unique tools to perform complex immunophenotyping, thereby enabling diagnostic procedures during early disease stages. Multicolor or polychromatic analysis of cells by SBC is of special importance not only as a cytomics technology platform but also because of low quantities of required reagents and biological material. The exact knowledge of the location of each cell on the slide permits repetitive restaining and reanalysis of specimens. Various separate measurements of the same specimen can be ultimately fused to one database increasing the information obtained per cell. Relocation and optical evaluation of cells as typical SBC feature, can be of integral importance for cytometric analysis, since artifacts can be excluded and morphology of measured cells can be documented. Progress in cell analytic: In the SBC, new horizons can be opened by the new techniques of structural and functional analysis with the high resolution from intracellular and membrane (confocal microscopy, nanoscopy, total internal fluorescence microscopy (TIRFM), and tissue level (tissomics), to organ and organism level (in vivo cytometry, optical whole body imaging). Predictive medicine aims at the detection of changes in patient's state prior to the manifestation of the disease or the complication. Such instances concern immune consequences of surgeries or noninfectious posttraumatic shock in intensive care patients or the pretherapeutic identification of high risk patients in cancer cytostatic therapy. Preventive anti‐infectious or anti‐shock therapy as well as curative chemotherapy in combination with stem cell transplantation may provide better survival chances for patient at concomitant cost containment. Predictive medicine‐guided optimization of therapy could lead to individualized medicine that gives significant therapeutic effect and may lower or abrogate potential therapeutic side effects. The 10th Leipziger Workshop combined with the 3rd International Workshop on SBC aimed to offer new methods in Image‐ and Slide‐Based Cytometry for solutions in clinical research. It moved towards practical applications in the clinics and the clinical laboratory. This development will be continued in 2006 at the upcoming Leipziger Workshop and the International Workshop on Slide‐Based Cytometry. © 2005 International Society for Analytical Cytology

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“…We believe that the plethora of available contrast mechanisms in the interaction of light and tissue [1] will ultimately allow for the rapid and non-invasive detection of cancerous and other disease states in vivo, by imaging without the use of contrast agents [2] , thus enabling better clinical intervention [3] . We have previously reported on preliminary work addressing the concept, implementation and clinical deployment of a spectral imaging endoscope focusing on detection of elastic (Mie) scattering from tissue, for diagnostic purposes [4] .…”

Section: Introductionmentioning

confidence: 99%

Quantitative endoscopic imaging elastic scattering spectroscopy: model system/tissue phantom validation

Lindsley

Farkas

2008

SPIE Proceedings

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We have designed and built an imaging elastic scattering spectroscopy endoscopic instrument for the purpose of detecting cancer in vivo. As part of our testing and validation of the system, known targets representing potential disease states of interest were constructed using polystyrene beads of known average diameter and TiO 2 crystals embedded in a two-layer agarose gel. Final construction geometry was verified using a dissection microscope. The phantoms were then imaged using the endoscopic probe at a known incident angle, and the results compared to model predictions. The mathematical model that was used combines classic ray-tracing optics with Mie scattering to predict the images that would be observed by the probe at a given physical distance from a Mie-regime scattering media. This model was used generate the expected observed response for a broad range of parameter values, and these results were then used as a library to fit the observed data from the phantoms. Compared against the theoretical library, the best matching signal correlated well with known phantom material dimensions. These results lead us to believe that imaging elastic scattering can be useful in detection/diagnosis, but further refinement of the device will be necessary to detect the weak signals in a real clinical setting.

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Advanced Optical Imaging Requiring No Contrast Agents—A New Armamentarium for Medicine and Surgery

Cited by 11 publications

References 18 publications

Cytomics – importance of multimodal analysis of cell function and proliferation in oncology

Cytomics – importance of multimodal analysis of cell function and proliferation in oncology

Systems biology and clinical cytomics: The 10th Leipziger workshop and the 3rd international workshop on slide‐based cytometry

Quantitative endoscopic imaging elastic scattering spectroscopy: model system/tissue phantom validation

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