Advanced non-small cell lung cancer: Rapid evaluation of EGFR status on fine-needle cytology samples using Idylla

D'Ardia, Angela; Caputo, Alessandro; Fumo, Rosalba; Ciaparrone, Chiara; Gaeta, Sara; Picariello, Caterina; Zeppa, Pio; D’Antonio, Antonio

doi:10.1016/j.prp.2021.153547

Cited by 15 publications

(16 citation statements)

References 18 publications

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“…Depending on the availability of material, scraped smears or CNB sections were utilized. Massively parallel sequencing was used for wide gene panel assessments (Cancer Panel, MiSeq, Illumina) while Idylla cartridges (Biocartis) were employed for single or limited mutations when appropriate 15,16,38–40 …”

Section: Methodsmentioning

confidence: 99%

“…The incorporation of rapid on‐site evaluation (ROSE) further enhances its utility by gauging sample adequacy, leading to a notable decrease of approximately 20% in inadequate rates 4,13 . The precision of FNAC's diagnostic outcomes is augmented through immunocytochemistry (ICC) on diverse substrates such as cell block (CB), liquid‐based cytology (LBC) or smears, along with molecular testing, 14–17 and flow cytometry (FC) for lymphoproliferative processes 6,10,18–24 …”

Section: Introductionmentioning

confidence: 99%

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Combined fine needle aspiration cytology and core needle biopsy in the same setting: A two‐years’ experience

Ciliberti,

Maffei,

D'Ardia

et al. 2023

Cytopathology

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IntroductionFine needle aspiration cytology (FNAC) combined with rapid on‐site evaluation (ROSE) and ancillary techniques is an accurate diagnostic tool for many pathologies. However, in some cases, it may not be sufficient for actionable diagnoses or molecular testing, especially for cases that require large immunohistochemical panels or cases in which histological features are mandatory for the diagnosis. Core needle biopsy (CNB), on the contrary, provides samples that are suitable for histological features and sufficient for all ancillary studies. However, CNB is often performed by radiologists or clinicians without the direct participation of cytopathologists, which can lead to missed or delayed diagnoses. This study reports on the experience of combining FNAC and CNB performed in one setting by cytopathologists. The aim was to evaluate the impact of CNB on FNAC and the diagnostic efficiency of the combined procedures.Materials and MethodsOne hundred forty‐two FNAC and CNB procedures performed in the same setting over a period of 2 years were analysed. The FNAC diagnoses were compared and integrated with the subsequent CNB diagnoses. The impact of CNB was categorized as follows: non‐contributory, in cases of inadequate samples; confirmed, when the CNB and FNAC diagnoses were the same; improved, when the CNB diagnosis was consistent with the FNAC diagnosis and further specified the corresponding entity; allowed, when CNB produced a diagnosis that could not be reached by FNAC; changed, when the CNB changed the previous FNAC diagnosis.ResultsCNB confirmed the FNAC diagnosis in 40.1% of cases (n = 57/142). CNB improved the FNAC diagnosis in 47.2% of cases (n = 67/142). CNB allowed a diagnosis that could not be performed on FNAC in 2.1% of cases (n = 3/142). CNB changed a previous FNAC diagnosis in 2.1% of cases (n = 3/142). CNB was non‐contributory in 8.4% of cases (n = 12/142). CNB produced a positive impact on the whole diagnostic procedure in 51.4% of total cases (n = 73/142). The combined FNAC and CNB resulted in actionable diagnoses in 91.5% of all cases (n = 130/142). A complete molecular assessment was successfully performed in 14.7% of cases (n = 21/142) utilizing either FNAC or CNB material.ConclusionsThe combined use of FNAC and CNB in one setting improves the diagnostic accuracy of both procedures. This approach exploits the advantages of each procedure, enhancing the accuracy of the final diagnosis.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Combined fine needle aspiration cytology and core needle biopsy in the same setting: A two‐years’ experience

Ciliberti,

Maffei,

D'Ardia

et al. 2023

Cytopathology

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“…Variations of this type of analysis make it possible to monitor things like tumor burden and minimal residual disease of a fusion using RNA transcripts. 47 These types of analysis have also been adopted into semi and fully‐automated platforms like Biocartis Idylla™ that can go from FFPE sample to result in approximately 2 h. 48 , 49 This is covered in detail in other reviews. 50 , 51 …”

Section: Analytical Methods Commonly Used In Cytopathologymentioning

confidence: 99%

The basics of commonly used molecular techniques for diagnosis, and application of molecular testing in cytology

Turner

Shaar

Yang

2022

Diagnostic Cytopathology

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Molecular diagnostics has expanded to become the standard of care for a variety of solid tumor types. With limited diagnostic material, it is often desirable to use cytological preparations to provide rapid and accurate molecular results. This review covers important pre‐analytic considerations and limitations, and a description of common techniques that the modern cytopathologist should understand when ordering and interpreting molecular tests in practice.

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“…FNAC is a safe, rapid, and relatively inexpensive method: it allows obtaining an amount of material sufficient to make a diagnosis with lower costs and distress for the patients, and is routinely used to solve diagnostic conundrums in the head and neck district [6,7,[15][16][17][18]. FNAC can also be used to obtain material for bacterial cultures, histochemistry, immunohistochemistry, and molecular tests [12,[19][20][21][22][23][24][25]. Ancillary histochemical stains may be helpful to distinguish Actinomyces from its bacterial or non-bacterial mimics: by using Gomori silver and Gram stains, Actinomyces (positive) can be distinguished from its fungal mimickers, while a positive acid-fast stain will point towards a Nocardia infection [12].…”

Section: N Case Reportsmentioning

confidence: 99%

Actinomycosis mimicking malignancy: a report of three cases diagnosed with fine-needle aspiration cytology

et al. 2022

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We describe three cases of actinomycosis of the head and neck area, clinically suspected to be malignancies, diagnosed by fine-needle aspiration (FNAC). The patients presented with painless, slowly growing masses in the cervicofacial area. Ultrasonography identified the masses as enlarged lymph nodes which were subsequently biopsied by FNAC. Cytological features were similar in all cases, with a background of granulocytes and scattered lymphocytes and histiocytes. At high magnification colonies of branching, filamentous and beaded bacteria were detected. In the Diff-Quikstained smears, these filamentous colonies showed an evident yellowish color with the typical feature of the "sulfur granules" consistent with the Splendore-Hoep-SUMMARY pli phenomenon. A diagnosis of actinomycosis was made and confirmed in all cases by the subsequent microbiological tests. The patients were treated with high-dose penicillin, which caused the masses to progressively shrink. The lymph nodal localization of cervico-facial actinomycosis may be a diagnostic challenge, because in that area, lymphadenopathies may occur both in benign and malignant conditions. FNAC is a safe, fast, and reliable method to perform an accurate diagnosis of actinomycosis avoiding the surgical excision for histological evaluation.

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Advanced non-small cell lung cancer: Rapid evaluation of EGFR status on fine-needle cytology samples using Idylla

Cited by 15 publications

References 18 publications

Combined fine needle aspiration cytology and core needle biopsy in the same setting: A two‐years’ experience

Combined fine needle aspiration cytology and core needle biopsy in the same setting: A two‐years’ experience

The basics of commonly used molecular techniques for diagnosis, and application of molecular testing in cytology

Actinomycosis mimicking malignancy: a report of three cases diagnosed with fine-needle aspiration cytology

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