Advanced methods in the evaluation of premalignant lesions and carcinomas of the oral mucosa

Burkhardt, A

doi:10.1111/j.1600-0714.1985.tb00467.x

Cited by 79 publications

(32 citation statements)

References 167 publications

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“…23 It is important to quantify these dysplastic changes in oral epithelium as the degree of dysplasia is limited to the degree of probability to the development of malignancy. 24 Damage to DNA is suggested to be a common mechanism for cancer initiation. Deoxyribonucleic acid damage especially that which is radiation induced has been assessed by comet assay, 20 but the use of this technique in cancer has been limited.…”

Section: Discussionmentioning

confidence: 99%

“…23 Careful study and analysis of the same may be useful to predict the chances of malignant transformation. 24 The stage at which oral cancer is diagnosed plays an important role in determining the mortality and morbidity following treatment. 29 For instance, for the early stage of the disease (Stage 1), there is an estimated 80% 5-year survival rate whereas for the advanced stage of the disease (Stage 4), the 5 years survival rate can be as low as 20%.…”

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confidence: 99%

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Assessment of DNA Damage in Leukoplakia Patients with Different Degrees of Dysplasia

Vellappally

Binmgren

Huraib

et al. 2015

The Journal of Contemporary Dental Practice

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Background: Single cell gel electrophoresis (SCGE) assay also known as comet assay is a rapid and highly sensitive fluorescent molecular technique for detecting various forms of deoxyribonucleic acid (DNA) damage at individual cellular level. Materials and methods:The present study was done to detect the extent of DNA damage in oral leukoplakia (OL) and compare with normal individuals. The sample population was obtained from an outpatient clinic of a tertiary teaching dental institute. A total of 36 consecutive patients with leukoplakia and 10 healthy normal volunteers were recruited for the study and assessed for the extent of DNA damage using SCGE following clinical diagnosis and histological grading. Peripheral blood was obtained by venipuncture and SCGE assay was performed. Mean comet tail length was recorded and analyzed statistically to compare the extent of damage in each group. Results:The mean comet tail length seen in leukoplakia patients with moderate to severe dysplasia was 1.25 ± 0.14 mm while for the control subjects, it was 0.31 ± 0.10 mm. The difference was statistically significant (p = 0.000). On comparing within the grades of leukoplakia, a progressive trend of increasing tail length was observed with increasing grades of dysplasia. Assessment of DNA Damage in Leukoplakia Patients Conclusion:Deoxyribonucleic acid damage as measured by SCGE is seen in leukoplakia. A stepwise increase in DNA damage levels from healthy controls, through patients with non-dysplastic epithelium to varying grades of dysplasia has been observed indicating the extent of DNA damage in this high risk group.

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Assessment of DNA Damage in Leukoplakia Patients with Different Degrees of Dysplasia

Vellappally

Binmgren

Huraib

et al. 2015

The Journal of Contemporary Dental Practice

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“…The cells of certain types of tumours may be more readily detached from each other than the cells of normal tissues (Collins, 1990;Burkhardt, 1980Burkhardt, , 1985. Therefore altered cell-cell adhesion may make an important contribution to the initiation of both invasive and metastatic spread of tumours.…”

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confidence: 99%

Immunohistochemical staining of desmosomal components in oral squamous cell carcinomas and its association with tumour behaviour

Hiraki

Shinohara²,

Ikebe³

et al. 1996

Br J Cancer

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Sumary Desmosomes are intercellular junctions that have been shown to be down-regulated in certain types of carcinomas and that may play a role in suppression of invasion and metastasis. We have shown previously that immunohistochemical staining for the major desmosomal glycoprotein, desmoglein (Dsg), is reduced in some cases of squamous cell carcinoma (SCC) of the head and neck, and that reduced staining correlates with lymph node involvement. Desmosomes are multicomponent organelles. We therefore sought to determine whether another major desmosomal mokcule, desmoplakin (Dp), showed similar reduced expression to that shown by desmoglein. We have stained 65 specimens of primary SCC of the oral cavity (37 non-metastatic and 28 metatastic) with monoclonal antibodies to both desmoglein and desmoplak-in. We show that reduction of Dp staining correlates with loss of differentiation of the primary tumour, degree of invasion and presence of lymph node metastases. Similar correlations were found with Dsg staining. There was also correlation between reduction in Dp staining and reduction in Dsg staining. It is concluded that down-regulation of desmosomal expression occurs in some cases of SCC of the oral cavity and is associated with invasion and metastasis. Desmosomes may have an invasion and metastasis suppressor function.

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“…These pre-neoplastic lesions are known to have the potential to progress to squamous cell tumors 20,26) . Maltoni et al 8) and Minardi et al 9) reported that the occurrence of squamous cell dysplasias in the esophagus and forestomach was closely associated with squamous cell carcinomas.…”

Section: Discussionmentioning

confidence: 99%

Carcinogenicity and Chronic Toxicity in Mice and Rats Administered Vinyl Acetate Monomer in Drinking Water

Umeda

Matsumoto

Yamazaki

et al. 2004

Journal of Occupational Health

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Carcinogenicity and Chronic Toxicity in Mice and Rats Administered Vinyl Acetate Monomer in Drinking Water: Yumi Umeda, et al. Japan Bioassay Research Center, Japan Industrial Safety and Health Association—Carcinogenicity and chronic toxicity of vinyl acetate monomer (VA) were examined in male and female Crj:BDF1 mice and F344/DuCrj Rats. Groups of 50 mice and 50 rats of each sex were orally administered VA in drinking water containing 0, 400, 2,000 or 10,000 ppm (g/g) VA for 104 wk. Squamous cell tumors were clearly evident in the upper digestive tract of treated mice and rats, and in the larynx of treated mice of both sexes. In mice, squamous cell carcinomas and papillomas were observed in the oral cavity, esophagus, forestomach and larynx of the 10,000 ppm group, together with basal cell hyperplasia, squamous cell hyperplasia and epithelial dysplasia. In rats, incidences of squamous cell carcinomas and papillomas were increased in the oral cavity of the 10,000 ppm group of both sexes, and an esophagus squamous cell carcinoma was observed in a 10,000 ppm female. Pre‐neoplastic hyperplasias were also noted. Mapping of the neoplastic and pre‐neoplastic lesions in the oral cavity of the 10,000 ppm group revealed that both the lesions occurred predominantly at Level V in mice and at Level VI in rats. A lower confidence limit of a benchmark dose (BMDL10) of 477 mg/kg/d was obtained from a dose‐response relationship between combined incidence of squamous cell carcinomas and papillomas in the oral cavity of mice and rats and the estimated daily VA intakes per body weight, and compared with literature values.

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Advanced methods in the evaluation of premalignant lesions and carcinomas of the oral mucosa

Cited by 79 publications

References 167 publications

Assessment of DNA Damage in Leukoplakia Patients with Different Degrees of Dysplasia

Assessment of DNA Damage in Leukoplakia Patients with Different Degrees of Dysplasia

Immunohistochemical staining of desmosomal components in oral squamous cell carcinomas and its association with tumour behaviour

Carcinogenicity and Chronic Toxicity in Mice and Rats Administered Vinyl Acetate Monomer in Drinking Water

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