Advanced method for determination of omeprazole in plasma by HPLC

Kang, Wonku; Kim, Dong-Sup; Kwon, Kwang‐il

doi:10.1007/bf02976443

Cited by 21 publications

(29 citation statements)

References 5 publications

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“…To obtain chromatograms with good separation various columns, mixtures of mobile phase and different flow rates were investigated. For the assay of PPIs in human plasma a ODS-A C 18 was found to be better than Nova-Pak C 18 or inertsil ODS-3V C 18 . Various mixtures of phosphate buffer/acetonitrile were used as mobile phase and results indicated that phosphate buffer (10 mM)/acetonitrile (53/47, adjusted pH to 7.3 with triethylamine) gives the best resolution.…”

Section: Resultsmentioning

confidence: 99%

“…Chromatographic separation of PPIs was achieved using a TRACER EXCEL 120 ODS-A (5 µm 250 mm×4.6 mm i.d, Teknokroma) reverse phase analytical column with a µBondapak™ C 18 Guard column (5 μm, 120 Aº, Ireland). The mobile phase consisting of a mixture of phosphate buffer (10 mM)/acetonitrile (53/47, v/v adjusted pH to 7.3 with triethylamine).…”

Section: Apparatus and Chromatographic Conditionsmentioning

confidence: 99%

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Improved HPLC Method for Determination of Four PPIs, Omeprazole, Pantoprazole, Lansoprazole and Rabeprazole in Human Plasma

et al. 2010

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-PURP OSE:To develop a simple and rapid HPLC method for measuring of four proton-pump inhibitors (PPIs), omeprazole (OPZ), pantoprazole (PPZ), lansoprazole (LPZ) and rabeprazole (RPZ) concentrations in human plasma. METHODS: Following a single step liquid-liquid extraction analytes along with an internal standard (IS) were separated using an isocratic mobile phase of phosphate buffer (10 mM)/acetonitrile (53/47, v/v adjusted pH to 7.3 with triethylamine) at flow rate of 1 mL/min on reverse phase TRACER EXCEL 120 ODS-A column at room temperature. RESULTS: Total analytical run time for selected PPIs was 10 min. The assays exhibited good linearity (r 2 >0.99) over the studied range of 20 to 2500 ng/mL for OPZ, 20 to 4000 ng/mL for PPZ, 20 to 3000 ng/mL for LPZ and 20 to 1500 ng/mL for RPZ. The recovery of method was equal or greater than 80% and lower limit of quantification (LLOQ) was 20 ng/mL for four PPIs. Coefficient of variation and error at all of the intra-day and inter-day assessment were less than 9.2% for all compounds. CONCLUSIONS: The results indicated that this method is a simple, rapid, precise and accurate assay for determination of four PPIs concentrations in human plasma. This validated method is sensitive and reproducible enough to be used in pharmacokinetic studies and also is time-and cost-benefit when selected PPIs are desired to be analyzed.

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Section: Resultsmentioning

confidence: 99%

Section: Apparatus and Chromatographic Conditionsmentioning

confidence: 99%

Improved HPLC Method for Determination of Four PPIs, Omeprazole, Pantoprazole, Lansoprazole and Rabeprazole in Human Plasma

et al. 2010

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“…For clinical pharmacology studies, a simple and rapid extraction step is necessary, which is why liquid-liquid extraction was chosen. This extraction method was also used by Kang et al, 1999;Wang et al, 2004;Desmet et al, 2010; but with higher limit of quantification by Bharathi et al, 2009A solid phase extraction of 90% efficiency was proposed by Woolf et al, 1998;Kanazawa et al, 2004 but it is more expensive than liquid-liquid extraction. The simple protein precipitation extraction procedure was investigated but proved to be unacceptable due to interference and low sensitivity.…”

Section: Application Of the Methods In Human Subjectsmentioning

confidence: 99%

“…Assay of omeprazole and omeprazole sulfone has been reported using semi-micro column liquid chromatography with mixed-function precolumn (Yim et al, 2001). Several HPLC methods have been published for quantitatively determining omeprazole levels in human plasma (Vittal et al, 2009;DeSmet et al, 2010;TsingHua 2004;Bharathi et al, 2009;Kanazawa et al, 2004;Wang et al, 2004 andKang et al, 1999). The methods are isocratic and gradient HPLC (or) HPLC with column switching liquid chromatography mass spectrometry (LC-MS/MS).…”

Section: Introductionmentioning

confidence: 99%

An Lc- MS/MS Method for the Determination of Omeprazole on Proton Pump Inhibitor in Human Plasma

Sudha¹,

Reddy²,

Hemalatha³

et al. 2016

Indonesian J. Pharm.

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A sensitive and selective liquid chromatographic method coupled with tandem mass spectroscopy (LC-MS/MS) was developed for the quantification of omeprazole in human plasma. Lansoprazole was used as internal standard with plasma samples, extracted using 10mM ammonium acetate. A centrifuged upper layer was then evaporated and reconstituted with Acetonitrile: mobile phase buffer 70:30%v/v. The reconstructed samples were injected into a C18 column purospher star 5µ. The mobile phase was composed of ACN: mobile phase buffer (5mm ammonium bicarbonate buffer) in the ratio of 70:30%v/v with flow rate 1.0mL/min. The mass spectrometer was operated using positive ion mode and turbo electro spray ionisation. Nitrogen was used as the nebulizer, curtain, collision and auxiliary gases. Using MS/MS with multiple reactions monitoring (MRM) mode, omeprazole was detected without severe interferences from plasma matrix. Detection of omeprazole in human plasma was accurate and precision. This method has been successfully applied to the study of omeprazole in human specimens

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“…The reaction was terminated by addition of 1 ml of diethyl ether after 5 min incubation. Omeprazole was measured by a reported HPLC method [14]. The kinetic constants (K m and V max ) for the disappearance of omeprazole were calculated using a non-linear regression method [15].…”

Section: Preparation Of Hepatic and Intestinal Microsomesmentioning

confidence: 99%

Faster clearance of omeprazole in mutant Nagase analbuminemic rats: possible roles of increased protein expression of hepatic CYP1A2 and lower plasma protein binding

Lee

Jung

Kim

et al. 2009

Biopharm & Drug Disp

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It is well known that there are various changes in the expression of hepatic and intestinal CYPs in mutant Nagase analbuminemic rats (NARs). It has been reported that the protein expression of hepatic CYP1A2 was increased, whereas that of hepatic CYP3A1 was not altered, and it was also found that the protein expression of the intestinal CYP1A subfamily significantly increased in NARs from our other study. In addition, in this study additional information about CYP changes in NARs was obtained; the protein expression of the hepatic CYP2D subfamily was not altered, but that of the intestinal CYP3A subfamily increased in NARs. Because omeprazole is metabolized via hepatic CYP1A1/2, 2D1, 3A1/2 in rats, it could be expected that the pharmacokinetics of omeprazole would be altered in NARs. After intravenous administration of omeprazole to NARs, the Cl(nr) was significantly faster than in the controls (110 versus 46.6 ml/min/kg), and this could be due to an increase in hepatic metabolism caused by a greater hepatic CYP1A2 level in addition to greater free fractions of the drug in NARs. After oral administration of omeprazole to NARs, the AUC was also significantly smaller (80.1% decrease) and F was decreased in NARs. This could be primarily due to increased hepatic and intestinal metabolism caused by greater hepatic CYP1A2 and intestinal CYP1A and 3A levels. In particular, the smaller F could mainly result from greater hepatic and intestinal first-pass effect in NARs than in the controls.

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Advanced method for determination of omeprazole in plasma by HPLC

Cited by 21 publications

References 5 publications

Improved HPLC Method for Determination of Four PPIs, Omeprazole, Pantoprazole, Lansoprazole and Rabeprazole in Human Plasma

Improved HPLC Method for Determination of Four PPIs, Omeprazole, Pantoprazole, Lansoprazole and Rabeprazole in Human Plasma

An Lc- MS/MS Method for the Determination of Omeprazole on Proton Pump Inhibitor in Human Plasma

Faster clearance of omeprazole in mutant Nagase analbuminemic rats: possible roles of increased protein expression of hepatic CYP1A2 and lower plasma protein binding

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