“…Calculation of bacteria using the calculation of the CFU value. While the TPH test is carried out by gravimetric analysis based on the weight of a substance [20]. TPH was extracted using 5 ml of hexane solution for 3 replications.…”
Section: Total Petroleum Hydrocarbon (Tph) Test and Environmental Par...mentioning
One of the mitigations in dealing with the problem of waste oil is by using bioremediation techniques. Bioremediation techniques use biological agents that are safe for the environment and more cost-effective. The method commonly developed in bioremediation techniques is the immobilization of bacteria using plant fibers. The plant fiber used is ketapang leaf (Terminalia catappa) because it has physico-chemical properties that can be used in bioremediation. This study aims to analyze the ability of T. catappa as a living medium for bacteria degrading oil waste and analyze the effect of storage of live media for bacteria degrading oil waste. Five bacterial isolates used in this study were Bacillus aquimaris, B. megaterium, B. pumilus, H. trueperi, and R. bacterium. The live media for waste oil-degrading bacteria was made from T. catappa powder. Tests were carried out on storage days 0, 7, and 28. The results showed that T. catappa leaves could be used as a living medium for bacteria that degrade waste oil (bio carrier) in the amount of 19.9%-55.8%.
“…Calculation of bacteria using the calculation of the CFU value. While the TPH test is carried out by gravimetric analysis based on the weight of a substance [20]. TPH was extracted using 5 ml of hexane solution for 3 replications.…”
Section: Total Petroleum Hydrocarbon (Tph) Test and Environmental Par...mentioning
One of the mitigations in dealing with the problem of waste oil is by using bioremediation techniques. Bioremediation techniques use biological agents that are safe for the environment and more cost-effective. The method commonly developed in bioremediation techniques is the immobilization of bacteria using plant fibers. The plant fiber used is ketapang leaf (Terminalia catappa) because it has physico-chemical properties that can be used in bioremediation. This study aims to analyze the ability of T. catappa as a living medium for bacteria degrading oil waste and analyze the effect of storage of live media for bacteria degrading oil waste. Five bacterial isolates used in this study were Bacillus aquimaris, B. megaterium, B. pumilus, H. trueperi, and R. bacterium. The live media for waste oil-degrading bacteria was made from T. catappa powder. Tests were carried out on storage days 0, 7, and 28. The results showed that T. catappa leaves could be used as a living medium for bacteria that degrade waste oil (bio carrier) in the amount of 19.9%-55.8%.
“…The efficacy of the immobilized moiety will largely depend on properties of the substrate on which it is immobilized. [35,39,40] Immobilization substrates either inorganic (e. g., zeolites, glass, activated charcoal, clay) or organic [e. g., agarose, natural, or synthetic polymers (chitosan, cellulose, gelatin, alginate)] must contain the above properties to enable proper functioning of a bioreactor. Organic substrates are abundant in nature and can be easily produced with desirable properties.…”
Section: Properties Of a Suitable Immobilization Carriermentioning
confidence: 99%
“…For a substrate to be suitable for immobilization, it should have high stability (chemical, biological, and physical), low toxicity, low cost of production, high regeneration capacity, and high loading capacity. The efficacy of the immobilized moiety will largely depend on properties of the substrate on which it is immobilized [35,39,40] . Immobilization substrates either inorganic (e. g., zeolites, glass, activated charcoal, clay) or organic [e. g., agarose, natural, or synthetic polymers (chitosan, cellulose, gelatin, alginate)] must contain the above properties to enable proper functioning of a bioreactor.…”
Section: Bacteria In Bioremediation Of Crude Oil Spillsmentioning
Immobilization of biological molecules and cells on nanofibers is widely used in many applications ranging from medical to environmental applications. Immobilization materials provide cells with protection and surface for adhesion which in turn increases their efficiency for a particular application and stability over a longer period. Bioremediation of oil spills has recently become popular since scientists have developed processes that rely on cost-effectiveness and efficacy in crude oil treatments. For improved and sustainable performance of bioremediation, the system requires the development of cost-effective carrier substrates which undergo slow biodegradability and present a limited negative impact on the environment. Immobilization of bacteria on electrospun polymeric fibers is a recent research development aided by advances in nanotechnology. This could revolutionize bioremediation, treating the problem of crude oil spill pollution. In this review, we discuss the use of electrospinning to manufacture nanofibers entrapping and encapsulating bacterial cells for effective crude oil spill bioremediation. We go further to explain the recent developments in nanofiber technology with special emphasis on the correlation between method of electrospinning and relevant morphology of the formed fibers.
“…15 Le et al use of cinder beats (CB), coconut fiber (CF), and polyurethane foam (PUF) as carriers for bacteria immobilization to degrade crude oil, the results showed the capacity and enhancement of the biofilm-forming PPB inserted in these biocarriers to biodegrade PAHs and crude oil. 16 Molecularly imprinted nanofibrous membranes prepared using environmentally friendly materials as the base solution have the advantages of being nontoxic, nonhazardous, high mechanical strength, good stability, and reusable, making them an ideal carrier for microbial immobilization. 17 In the process of microbial degradation of oily wastewater, microorganisms use petroleum hydrocarbons as a carbon source to provide energy for their growth and reproduction, and after a series of their own metabolic reactions.…”
Combining molecular imprinting technology with electrostatic spinning technology, a molecularly imprinted nanofiber membrane material with memory and recognition of specific imprinted molecules is thus produced. It has the advantages of high efficiency, reusability, easy amplification, and simple operation. In this study, alkane molecularly imprinted nanofibrous membranes are prepared as a carrier for immobilize Bacillus cereus LY‐1 for the degradation of diesel in oil–water system. The adsorption capacity of the molecularly imprinted material for diesel oil with n‐decyl alcohol as the template molecule reach 120.25 mg g−1, the adsorption capacity of alkane molecularly imprint nanofiber membrane prepared using polyvinyl alcohol (PVA) as the membrane substrate material is 41.37 mg g−1. After 4 days, the immobilized LY‐1 cells eliminate roughly 80.7% of the diesel at a starting concentration of 3 g L−1. The results show that imprinted structures in MINM enhance degradation by loaded microorganisms.
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