Advanced human developmental toxicity and teratogenicity assessment using human organoid models

Li, Minghui; Gong, Jing; Gao, Lixiong; Zou, Ting; Kang, Jiahui; Xu, Haiwei

doi:10.1016/j.ecoenv.2022.113429

Cited by 39 publications

(21 citation statements)

References 252 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These are constituted by multiple tissues fabricated using two or more tissue chips or by incorporating multiple interconnected chambers representative of tissues/organs on one chip to recapitulate communications among different tissues [ 153 ]. Integrated multiorganoid models have been proposed in an effort to mimic complex procedures of metabolism and responses at the multiorganoid level, where flow rates resemble blood circulation in the human body [ 176 ]. Therefore, MOoCs can be exploited to evaluate the systemic toxicity of nanomaterials from the dynamic process of distribution, absorption, metabolism, and excretion features; nonetheless, systemic predictions are still challenging [ 157 , 158 ].…”

Section: Advanced Models For In Vitro Testingmentioning

confidence: 99%

Nanosafety: An Evolving Concept to Bring the Safest Possible Nanomaterials to Society and Environment

et al. 2022

View full text Add to dashboard Cite

The use of nanomaterials has been increasing in recent times, and they are widely used in industries such as cosmetics, drugs, food, water treatment, and agriculture. The rapid development of new nanomaterials demands a set of approaches to evaluate the potential toxicity and risks related to them. In this regard, nanosafety has been using and adapting already existing methods (toxicological approach), but the unique characteristics of nanomaterials demand new approaches (nanotoxicology) to fully understand the potential toxicity, immunotoxicity, and (epi)genotoxicity. In addition, new technologies, such as organs-on-chips and sophisticated sensors, are under development and/or adaptation. All the information generated is used to develop new in silico approaches trying to predict the potential effects of newly developed materials. The overall evaluation of nanomaterials from their production to their final disposal chain is completed using the life cycle assessment (LCA), which is becoming an important element of nanosafety considering sustainability and environmental impact. In this review, we give an overview of all these elements of nanosafety.

show abstract

Section: Advanced Models For In Vitro Testingmentioning

confidence: 99%

Nanosafety: An Evolving Concept to Bring the Safest Possible Nanomaterials to Society and Environment

et al. 2022

View full text Add to dashboard Cite

show abstract

“…Conventional twodimensional (2D) cell cultures can not recapitulate cell-cell interactions, animal models, and fully reflect human physiological processes due to species differences (Liu, Lin et al, 2020). Recently, with the rapid development of stem cell technology, stem cell-derived human organoids have been used for drug discovery, chemical toxicity, and safety in vitro assessment (Li M et al, 2021;Zeng, Li et al, 2021;Li et al, 2022a;Li et al, 2022b). Compared with 2D models, 3D organoid models are more suitable for comprehensively representing in vivo physiological microenvironments and cellular interactions (Azhdarzadeh, Saei et al, 2015;Prasad, Kumar et al, 2021).…”

Section: The Human 3d Modelmentioning

confidence: 99%

Nanoparticles in ocular applications and their potential toxicity

Cao

Yang

et al. 2022

Front. Mol. Biosci.

Self Cite

View full text Add to dashboard Cite

Nanotechnology has been developed rapidly in recent decades and widely applied in ocular disease therapy. Nano-drug delivery systems overcome the bottlenecks of current ophthalmic drug delivery and are characterized with strong biocompatibility, stability, efficiency, sustainability, controllability, and few side effects. Nanoparticles have been identified as a promising and generally safe ophthalmic drug-delivery system based on the toxicity assessment in animals. Previous studies have found that common nanoparticles can be toxic to the cornea, conjunctiva, and retina under certain conditions. Because of the species differences between humans and animals, advanced in vitro cell culture techniques, such as human organoids, can mimic the human organism to a certain extent, bringing nanoparticle toxicity assessment to a new stage. This review summarizes the advanced application of nanoparticles in ocular drug delivery and the potential toxicity, as well as some of the current challenges and future opportunities in nanotoxicological evaluation.

show abstract

“…[102][103][104] However, the lack of essential cell types (e.g., microglia and "vascular" cells), interphotoreceptor matrix, and retinal pigment epithelium (RPE)-PRC interaction limits the maturation of retinal organoids, which impedes predictive research on retinal development, function, and drug response. [105][106][107][108] Recently, the retinal organoids-on-chips have emerged as a new tool to mimic ocular microenvironmentrelated events and thereby enhance organoid growth and differentiation with a well-defined morphology.…”

Section: Retinal Organoids-on-a-chipmentioning

confidence: 99%

Microfluidic Techniques for Next‐Generation Organoid Systems

Gong

Kang

et al. 2022

Adv Materials Inter

Self Cite

View full text Add to dashboard Cite

PSCs (iPSCs) and embryonic stem cells (ESCs), or adult stem cells (ASCs). Pioneered in the Yoshiki Sasai lab, where cortical tissues and optic-cuplike structures were firstly induced from ESCs in vitro, [1,2] organoid technology is regarded as a milestone in the stem cell field because it cast light upon the path to stably growing self-aggregating and selfrenewing stem cells into native tissues by mimicking developmental processes. [3] Subsequently, the rapidly developing field of organoid technology is advancing the maturity of technology for developing 3D macrostructures of organs such as the retina, [4] heart, [5] liver, [6] kidney, [7] and intestines. [8] In marked contrast to traditional 2D monocultures of cell lines losing cell-cell and cell-matrix interactions, organoids maintain and define in situ phenotypes fairly well. Despite organoids having shown great progress in tissue morphogenesis and organogenesis, the lack of organ-specific biochemical and physical microenvironments and nonphysiological shape and size have limited the function and real-life applications of organoids. Therefore, innovative strategies are urgently needed to precisely control perfusion conditions, nutrient supply, and mechanical cues in order to establish an optimal microenvironment for organoid cultures.The recently developed organs-on-a-chip (OOC) technology provides an in vivo-like microenvironment, showing great potential to enhance our understanding of organ development, physiology, and pathology. [9] Besides, the application of dynamic flow conditions or mechanical stimuli provided by OOCs can improve the terminal maturation of cells. [10,11] OOCs usually are comprised of specialized cell types and tissue elements (biochemical and biophysical cues of in vivo microenvironment), aiming to capture key functions of a specific organ. [12] Organoids rely on the self-organization of growing stem cell aggregates, whereas OOC is based on a reductionist engineering approach. In addition, organoids are multicellular 3D tissue with an architecture resembling some aspects of original tissues, which are not classified as OOC because of their production through stochastic self-organization from PSCs or ASCs and lack of microenvironment elements. [13] However, organoid and OOC techniques each have their own advantages, and they can be appropriately combined to enable highfidelity human modeling from stem cells. [14] Such synergistic Organoids are 3D multicellular structures derived from pluripotent stem cells (PSCs) or adult stem cells (ASCs), which have attracted increasing interest in the fields of drug screening, cell therapy, and regenerative medicine. Despite considerable success in culturing organoids with native microanatomy, challenges to achieving a physiologically relevant microenvironment remain. Complex dynamic feedback between cells and the extracellular matrix and uncontrollable mechano-physiological cues hamper the further study of organoid systems. Innovative engineering approaches are needed to produce, control, and analy...

show abstract

Advanced human developmental toxicity and teratogenicity assessment using human organoid models

Cited by 39 publications

References 252 publications

Nanosafety: An Evolving Concept to Bring the Safest Possible Nanomaterials to Society and Environment

Nanosafety: An Evolving Concept to Bring the Safest Possible Nanomaterials to Society and Environment

Nanoparticles in ocular applications and their potential toxicity

Microfluidic Techniques for Next‐Generation Organoid Systems

Contact Info

Product

Resources

About