Advanced glycated albumin impairs HDL anti-inflammatory activity and primes macrophages for inflammatory response that reduces reverse cholesterol transport

Age-related accumulation of advanced glycation end-products-albumin, S100β, and the expressions of advanced glycation end product receptor differ in visceral and subcutaneous fat

“…AGE-albumin is serum albumin modified by AGEs [4], and its interaction with RAGE primes macrophage for inflammatory response and aggravates inflammation-related diseases [4].…”

N-Acetyl Cysteine Attenuated the Deleterious Effects of Advanced Glycation End-Products on the Kidney of Non-Diabetic Rats

“…Body weight (BW; g) was monitored weekly and percentual body weight gain was calculated using the following equation: (initial BW -final BW)/initial BW. Advanced glycated albumin preparation Rat serum albumin modified by advanced glycation (AlbAGE) was prepared as previously described [22]. Briefly, fatty acid and endotoxin free rat serum albumin (Sigma-Aldrich, Taufkirche, Germany) was incubated in vitro, for 4 days, under nitrogen atmosphere, at 37 °C, in the dark, under sterile conditions in a water bath shaker, with 10 mM glycoaldehyde (Sigma) dissolved in phosphate buffered saline (PBS) and EDTA (pH 7.4).…”

Efeito das gorduras interesterificadas sobre o desenvolvimento da lesão aterosclerótica em camundongos knockout para o receptor de LDL

“…Considerando que as alterações inflamatórias em macrófagos não necessariamente refletem a resposta inflamatória na parede arterial, já que outros componentes celulares, tais como linfócitos, neutrófilos, células musculares lisas e endoteliais, também estão envolvidos nesse processo, foram determinados a expressão e o conteúdo proteico de citocinas na aorta de camundongos, conforme protocolo validado em outros estudos (Dinarello, 2009;Hansson e Hermansson, 2011 (Okuda et al, 2012;Zhao et al, 2013). Adicionalmente, observou-se que a atividade dos receptores ABCA-1 e ABCG-1 foi prejudicada, pois o grupo PALM INTER apresentou redução no efluxo de colesterol para apo-AI e HDL 2 (Figura 15).…”