Advanced genes expression pattern greatly contributes to divergence in Verticillium wilt resistance between Gossypium barbadense and Gossupium hirsutum

Abstract

Verticillium, representing one of the world’s major pathogens, causes Verticillium wilt in important woody species, ornamentals, agricultural, etc., consequently resulting in a serious decline in production and quality, especially in cotton. Gossupium hirutum and Gossypium barbadense are two kinds of widely cultivated cotton species that suffer from Verticillium wilt, while G. barbadense has much higher resistance toward it than G. hirsutum. However, the molecular mechanism regarding their divergence in Verticillium wilt resistance remains largely unknown. In the current study, G. barbadense cv. Hai7124 and G. hirsutum acc. TM-1 were compared at 0, 12, 24, 48, 72, 96, 120, and 144 h post-inoculation (hpi) utilizing high throughput RNA-Sequencing. As a result, a total of 3,549 and 4,725 differentially expressed genes (DEGs) were identified, respectively. In particular, the resistant type Hai7124 displayed an earlier and faster detection and signaling response to the Verticillium dahliae infection and demonstrated higher expression levels of defense-related genes over TM-1 with respect to transcription factors, plant hormone signal transduction, plant-pathogen interaction, and nucleotide-binding leucine-rich repeat (NLR) genes. This study provides new insights into the molecular mechanisms of divergence in Verticillium wilt resistance between G. barbadense and G. hirsutum and important candidate genes for breeding V. dahliae resistant cotton cultivars.

Pan-genome Analysis of GT64 Gene Family and Expression Response to Verticillium wilt in Cotton

“…In G. barbadense, GBGT64_2 and GBGT64_4 may participate in the response to FOV treatment. Furthermore, utilizing transcriptome data [34], we observed that in G. hirsutum, the MEturquoise module was signi cantly positively correlated with post-inoculation at 144 h, while in G. barbadense, the MEturquoise module was signi cantly positively correlated with post-inoculation at 72 h. This may suggest that GHGT64_4 initiated immune response to Verticillium wilt earlier in G. barbadense compared to G. hirsutum.…”

“…Transcription analysis GHGT64_4 between G. hirsutum and G. barbadense Although a series of bioinformatics analyses have been conducted on the GT64 gene family and we have gained a basic understanding, their potential role in resistance to Verticillium wilt in upland and island cotton is still unclear. Based on the transcriptome data of 90 published samples [34] (derived from TM-1 and Hai7124 at time points 0 h, 12 h, 24 h, 48 h, 72 h, 96 h, 120 h, 144 h pre-and post-inoculation). TM-1 is a variety with low susceptibility to the disease, while Hai7124 is disease-resistant.…”

“…By utilizing transcriptome data from two cotton varieties [34], WGCNA analysis was performed with the WGCNA package (version 4.1.1). Upon threshold selection, a power function was applied to the scale-free relationship matrix with β = 5 for G. hirsutum and β = 7 for G. barbadense, resulting in an unscaled adjacency matrix.…”

UDP-glucosyltransferase 71C4 regulates seed development by redistributing phenylpropanoid metabolism in cotton

“…Raw data converted from the Illumina platform was subjected to quality control before subsequent analysis. The transcriptome analysis method was as described previously (He et al, 2022).…”

Transcriptomic datasets of Verticillium wilt resistant and non-resistant Gossypium barbadense varieties during pathogen inoculation

“…This susceptibility leads to a substantial reduction in both fiber quality and yield 14 . Notably, G. barbadense demonstrates high resistance or immunity to cotton V. dahliae , making it a valuable resource for identifying key genes associated with Verticillium wilt resistance and uncovering the corresponding regulatory mechanisms 15 , 16 . However, the lack of V. dahliae -susceptible G. barbadense germplasm resources has limited the research focus to comparing V. dahliae- resistant G. barbadense with either V. dahliae -resistant G. hirsutum or V. dahliae -susceptible G. hirsutum varieties 17 – 22 .…”