Advanced gelatin-based vascularization bioinks for extrusion-based bioprinting of vascularized bone equivalents

Leucht, A.; Volz, Ann‐Cathrin; Rogal, Julia; Borchers, Kirsten; Kluger, Petra J.

doi:10.1038/s41598-020-62166-w

Cited by 94 publications

(82 citation statements)

References 58 publications

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“…Gelatin alone is usually used as a sacrificial material that liquifies at 37 °C, but GelMA is a stronger material crosslinked irreversibly by UV. [119] Within MPSs, it may be a promising material for lymphangiogenesis-or angiogenesis-based vascularization methods, but encapsulating ECs within the material would require careful tuning of UV dosage to ensure that the encapsulated cells can still perform vasculogenesis following exposure. Interestingly, many reports detail the tuning of Gel-MA's stiffness via addition of other polymers [120,121] or adjustment of the material handling, [122][123][124] which shows potential translation to tissue-specific MPSs such as vascularized bone or tumor models, or soft-tissue MPSs replicating fat, lymphatic, or nerve tissues.…”

Section: Other Hydrogel Materials In Vascularized Mpss: a Brief Perspmentioning

confidence: 99%

Engineering New Microvascular Networks On‐Chip: Ingredients, Assembly, and Best Practices

Tronolone

Jain

2021

Adv Funct Materials

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Tissue engineered grafts show great potential as regenerative implants for diseased or injured tissues within the human body. However, these grafts suffer from poor nutrient perfusion and waste transport, thus decreasing their viability post-transplantation. Graft vascularization is therefore a major area of focus within tissue engineering because biologically relevant conduits for nutrient and oxygen perfusion can improve viability post-implantation. Many researchers used microphysiological systems as testing platforms for potential grafts owing to an ability to integrate vascular networks as well as biological characteristics such as fluid perfusion, 3D architecture, compartmentalization of tissue-specific materials, and biophysical and biochemical cues. Although many methods of vascularizing these systems exist, microvascular self-assembly has great potential for bench-to-clinic translation as it relies on naturally occurring physiological events. In this review, the past decade of literature is highlighted, and the most important and tunable components yielding a self-assembled vascular network on chip are critically discussed: endothelial cell source, tissue-specific supporting cells, biomaterial scaffolds, biochemical cues, and biophysical forces. This paper discusses the bioengineered systems of angiogenesis, vasculogenesis, and lymphangiogenesis and includes a brief overview of multicellular systems. It concludes with future avenues of research to guide the next generation of vascularized microfluidic models.

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Section: Other Hydrogel Materials In Vascularized Mpss: a Brief Perspmentioning

confidence: 99%

Engineering New Microvascular Networks On‐Chip: Ingredients, Assembly, and Best Practices

Tronolone

Jain

2021

Adv Funct Materials

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“…Takei et al have shown that the bFGF releasing PLA‐gelatin scaffold results in significant enhancement of angiogenesis and further remarkable hepatocyte survival in comparison with bFGF‐free scaffolds. In this study, bFGF‐containing scaffolds showed no adverse effect on engrafted hepatocytes because the glycogen storage of cells remained unchanged (Leucht, Volz, Rogal, Borchers, & Kluger, 2020).…”

Section: Signaling Moleculesmentioning

confidence: 78%

“…Banfi et al (2012), Chiu and Radisic (2010), Kant and Coulombe (2018), Langenfeld and Langenfeld (2004), Leucht et al (2020), Lovett et al (2009), Milkiewicz et al (2006), Rouwkema and Khademhosseini (2016), Rouwkema et al (2008), Saik et al (2011), Salybekov et al (2018), Vijayan et al (2019), and…”

Section: Signaling Moleculesmentioning

confidence: 99%

Signaling molecules orchestrating liver regenerative medicine

Zakeri

Mirdamadi

Kalhori

et al. 2020

J Tissue Eng Regen Med

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The liver is in charge of more than 500 functions in the human body, which any damage and failure to the liver can significantly compromise human life. Numerous studies are being carried out in regenerative medicine, as a potential driving force, toward alleviating the need for liver donors and fabrication of a 3D‐engineered transplantable hepatic tissue. Liver tissue engineering brings three main factors of cells, extracellular matrix (ECM), and signaling molecules together, while each of these three factors tries to mimic the physiological state of the tissue to direct tissue regeneration. Signaling molecules play a crucial role in directing tissue fabrication in liver tissue engineering. When mimicking the natural in vivo process of regeneration, it is tightly associated with three main phases of differentiation, proliferation (progression), and tissue maturation through vascularization while directing each of these phases is highly regulated by the specific signaling molecules. The understanding of how these signaling molecules guide the dynamic behavior of regeneration would be a tool for further tailoring of bioengineered systems to help the liver regeneration with many cellular, molecular, and tissue‐level functions. Hence, the signaling molecules come to aid all these phases for further improvements toward the clinical use of liver tissue engineering as the goal.

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“…The addition of other component of the ECM such as fibronectin, collagens, or glycosaminoglycans in the hydrogels could enable the production of even more biomimetic platforms. Moreover, gelatin has shown its potential in the creation of vascularized tissues through bioprinting technologies [ 229 ] that might help in the production of vascularized tumors. Also, in the last years new efforts have been made to study the role of the immune system in the tumor progression.…”

Section: Engineering the Tme Using Protein‐based Hydrogelsmentioning

confidence: 99%

Proteinaceous Hydrogels for Bioengineering Advanced 3D Tumor Models

et al. 2021

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The establishment of tumor microenvironment using biomimetic in vitro models that recapitulate key tumor hallmarks including the tumor supporting extracellular matrix (ECM) is in high demand for accelerating the discovery and preclinical validation of more effective anticancer therapeutics. To date, ECM‐mimetic hydrogels have been widely explored for 3D in vitro disease modeling owing to their bioactive properties that can be further adapted to the biochemical and biophysical properties of native tumors. Gathering on this momentum, herein the current landscape of intrinsically bioactive protein and peptide hydrogels that have been employed for 3D tumor modeling are discussed. Initially, the importance of recreating such microenvironment and the main considerations for generating ECM‐mimetic 3D hydrogel in vitro tumor models are showcased. A comprehensive discussion focusing protein, peptide, or hybrid ECM‐mimetic platforms employed for modeling cancer cells/stroma cross‐talk and for the preclinical evaluation of candidate anticancer therapies is also provided. Further development of tumor‐tunable, proteinaceous or peptide 3D microtesting platforms with microenvironment‐specific biophysical and biomolecular cues will contribute to better mimic the in vivo scenario, and improve the predictability of preclinical screening of generalized or personalized therapeutics.

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Advanced gelatin-based vascularization bioinks for extrusion-based bioprinting of vascularized bone equivalents

Cited by 94 publications

References 58 publications

Engineering New Microvascular Networks On‐Chip: Ingredients, Assembly, and Best Practices

Engineering New Microvascular Networks On‐Chip: Ingredients, Assembly, and Best Practices

Signaling molecules orchestrating liver regenerative medicine

Proteinaceous Hydrogels for Bioengineering Advanced 3D Tumor Models

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