Advanced diagnostic methods for identification of bacterial foodborne pathogens: contemporary and upcoming challenges

Panwar, Surbhi; Duggirala, K. Siddaardha; Yadav, Pooja; Debnath, Nabendu; Yadav, Ashok Kumar; Kumar, Ashwani

doi:10.1080/07388551.2022.2095253

Cited by 27 publications

(21 citation statements)

References 129 publications

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“…These include, inter alia, diagnostics, 1,2 biotechnology, 3,4 and quality control for food. 5,6 Traditional culture tests for the identification of bacteria are time-consuming and require highly trained personnel, thus producing immense costs and temporal delays. 7 In addition, the differentiation between very similar bacteria of one genus typically requires additional analyses, such as microscopy or antibody tests.…”

Section: Introductionmentioning

confidence: 99%

“…The detection and quantification of pathogens such as bacteria is one of the most important tasks in health care, as bacterial infections are a major cause of illness and death all over the world. These include, inter alia, diagnostics, , biotechnology, , and quality control for food. , Traditional culture tests for the identification of bacteria are time-consuming and require highly trained personnel, thus producing immense costs and temporal delays . In addition, the differentiation between very similar bacteria of one genus typically requires additional analyses, such as microscopy or antibody tests .…”

Section: Introductionmentioning

confidence: 99%

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Selective Quantification of Bacteria in Mixtures by Using Glycosylated Polypyrrole/Hydrogel Nanolayers

Balser,

Röhrl,

Spormann

et al. 2024

ACS Appl. Mater. Interfaces

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Here, we present a covalent nanolayer system that consists of a conductive and biorepulsive base layer topped by a layer carrying biorecognition sites. The layers are built up by electropolymerization of pyrrole derivatives that either carry polyglycerol brushes (for biorepulsivity) or glycoside moieties (as biorecognition sites). The polypyrrole backbone makes the resulting nanolayer systems conductive, opening the opportunity for constructing an electrochemistry-based sensor system. The basic concept of the sensor exploits the highly selective binding of carbohydrates by certain harmful bacteria, as bacterial adhesion and infection are a major threat to human health, and thus, a sensitive and selective detection of the respective bacteria by portable devices is highly desirable. To demonstrate the selectivity, two strains of Escherichia coli were selected. The first strain carries type 1 fimbriae, terminated by a lectin called FimH, which recognizes α-d-mannopyranosides, which is a carbohydrate that is commonly found on endothelial cells. The otherE. coli strain was of a strain that lacked this particular lectin. It could be demonstrated that hybrid nanolayer systems containing a very thin carbohydrate top layer (2 nm) show the highest discrimination (factor 80) between the different strains. Using electrochemical impedance spectroscopy, it was possible to quantify in vivo the type 1-fimbriated E. coli down to an optical density of OD600 = 0.0004 with a theoretical limit of 0.00005. Surprisingly, the selectivity and sensitivity of the sensing remained the same even in the presence of a large excess of nonbinding bacteria, making the system useful for the rapid and selective detection of pathogens in complex matrices. As the presented covalent nanolayer system is modularly built, it opens the opportunity to develop a broad band of mobile sensing devices suitable for various field applications such as bedside diagnostics or monitoring for bacterial contamination, e.g., in bioreactors.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Selective Quantification of Bacteria in Mixtures by Using Glycosylated Polypyrrole/Hydrogel Nanolayers

Balser,

Röhrl,

Spormann

et al. 2024

ACS Appl. Mater. Interfaces

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“…The growth of undesirable microorganisms in fruit and vegetables is an important issue, since they reduce the food shelf-life and increase the risk to consumers of foodborne illness. To confront this problem, the food industry has developed various technologies such as thermal processing, drying, freezing, refrigeration, irradiation and modified atmosphere [ 1 ]. However, these technologies cannot be applied in certain products.…”

Section: Introductionmentioning

confidence: 99%

Development and Evaluation of the Properties of Active Films for High-Fat Fruit and Vegetable Packaging

et al. 2023

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β-cyclodextrin and allyl isothiocyanate inclusion complexes (β-CD:AITC) have been proposed for developing fresh fruit and vegetable packaging materials. Therefore, the aim of this research was to develop active materials based on poly(lactic acid) (PLA) loaded with β-CD:AITC and to assess changes in the material properties during the release of AITC to food simulants. PLA films with 0, 5 and 10 wt.% β-CD:AITC were developed by extrusion. Surface properties were determined from contact angle measurements. Films were immersed in water, aqueous and fatty simulants to assess the absorption capacity and the change in the thermal properties. Moreover, the release of AITC in both simulants was evaluated by UV-spectroscopy and kinetic parameters were determined by data modeling. Results showed that a higher concentration of β-CD:AITC increased the absorption of aqueous simulant of films, favoring the plasticization of PLA. However, the incorporation of β-CD:AITC also avoided the swelling of PLA in fatty simulant. These effects and complex relationships between the polymer, inclusion complexes and food simulant explained the non-systematic behavior in the diffusion coefficient. However, the lower partition coefficient and higher percentage of released AITC to the fatty simulant suggested the potential of these materials for high-fat fruit and vegetable active packaging applications.

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“…PCR methods are typically quick (3–6 h) and sensitive but demand intensive nucleic acid extraction techniques along with relatively costly equipment, while immunological assays maintain a significantly lower sensitivity (10 3 –10 5 colony-forming unit or CFU/ml; Wang et al, 2017 ). Traditional methods are not suited for high-throughput screening of large food samples for the presence of one or multiple foodborne pathogens ( Jasson et al, 2010 ; Hegde et al, 2012 ; Panwar et al, 2022 ). As a result, a robust, inexpensive, and rapid detection system, like on-site and portable biosensors, is needed to guarantee consumer food safety.…”

Section: Introductionmentioning

confidence: 99%

Advances, applications, and limitations of portable and rapid detection technologies for routinely encountered foodborne pathogens

et al. 2022

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Traditional foodborne pathogen detection methods are highly dependent on pre-treatment of samples and selective microbiological plating to reliably screen target microorganisms. Inherent limitations of conventional methods include longer turnaround time and high costs, use of bulky equipment, and the need for trained staff in centralized laboratory settings. Researchers have developed stable, reliable, sensitive, and selective, rapid foodborne pathogens detection assays to work around these limitations. Recent advances in rapid diagnostic technologies have shifted to on-site testing, which offers flexibility and ease-of-use, a significant improvement from traditional methods’ rigid and cumbersome steps. This comprehensive review aims to thoroughly discuss the recent advances, applications, and limitations of portable and rapid biosensors for routinely encountered foodborne pathogens. It discusses the major differences between biosensing systems based on the molecular interactions of target analytes and biorecognition agents. Though detection limits and costs still need further improvement, reviewed technologies have high potential to assist the food industry in the on-site detection of biological hazards such as foodborne pathogens and toxins to maintain safe and healthy foods. Finally, this review offers targeted recommendations for future development and commercialization of diagnostic technologies specifically for emerging and re-emerging foodborne pathogens.

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Advanced diagnostic methods for identification of bacterial foodborne pathogens: contemporary and upcoming challenges

Cited by 27 publications

References 129 publications

Selective Quantification of Bacteria in Mixtures by Using Glycosylated Polypyrrole/Hydrogel Nanolayers

Selective Quantification of Bacteria in Mixtures by Using Glycosylated Polypyrrole/Hydrogel Nanolayers

Development and Evaluation of the Properties of Active Films for High-Fat Fruit and Vegetable Packaging

Advances, applications, and limitations of portable and rapid detection technologies for routinely encountered foodborne pathogens

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