Advanced backcross quantitative trait locus analysis in winter wheat: Dissection of stripe rust seedling resistance and identification of favorable exotic alleles originated from a primary hexaploid wheat (Triticum turgidum ssp. dicoccoides × Aegilops tauschii)

Naz, Ali Ahmad; Kunert, A.; Flath, Kerstin; Pillen, Klaus; Léon, Jens

doi:10.1007/s11032-012-9710-2

Cited by 13 publications

(7 citation statements)

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“…QTL analysis was performed according to Naz et al. () using a multiple QTL model iteratively by forward selection/backward elimination using proc mixed procedure in sas software (SAS (2004) The SAS system for windows, release 9.2, SAS Institute, Cary, N.C. U.S.A). In this multiple QTL model, a following iteration was started when more QTL were detected then iteration rounds were performed.…”

Section: Methodsmentioning

confidence: 99%

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Genetic analysis and detection of new QTL alleles for Septoria tritici blotch resistance using two advanced backcross wheat populations

et al. 2015

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Septoria tritici blotch (STB) is the most aggressive disease that plagues wheat production across the world. In this study, we utilized two advanced backcross populations designated as B22 and Z86 to quest new genetic resources for STB field resistance. The populations B22 and Z86 were derived by crossing winter wheat cultivars 'Batis' and 'Zentos' with unique primary (exotic) hexaploid wheat accessions Syn022L and Syn086L, respectively. QTL analysis revealed ten QTL in the population B22 of which at five loci the introgression of exotic alleles resulted in an increase in STB resistance. The strongest exotic QTL allele was detected on chromosome 2D which decreases disease severity by 20.8%. In population Z86, five QTL were identified of which three resulted in decreasing STB severity due to the introgression of exotic alleles. Here, the strongest QTL QStb.Z86.3A was found on chromosome 3A which decreases disease severity by 19.7%. In both populations, 53% of the exotic alleles were associated to decrease STB severity suggesting the utility of primary wheat gene pool in questing new resistance resources in wheat.Key words: Septoria tritici blotch -QTL analysis -synthetic hexaploid wheat accessions Bread wheat (Triticum aestivum L.) is the major grain crop that feeds billions of humans and used as staple food for 35% of the world population. Fungal diseases are the major threats to its productivity and food security. Among fungal disease, Septoria tritici blotch (STB) is one of the most destructive diseases that affects vegetative and reproductive growth of wheat plant (Eyal and Levy 1987, Ghaffary et al. 2011). The severe epidemics of this disease can cause serious yield losses up to 60% especially in the area of frequent rain and lower night temperature in a growing season (Cornish et al. 1990). STB is caused by ascomycete fungus Zymoseptoria tritici (Desm.) Quaedvlieg & Crous, comb. nov. [syn. Septoria tritici Desm.; teleomorph Mycosphaerella graminicola (Fuckel) J. Schr€ ot.] which has been considered as one of the most aggressive and long-lasting pathogen of wheat (Stukenbrock et al. 2010, Miedaner et al. 2014). The pathogen spreads through airborne ascospores, infested crop debris and through volunteer wheat and related susceptible grass species. It has been reported that STB pathogens showed ability to develop resistance against some fungicides like azole (Cools and Fraaije 2008). Therefore, development of resistant cultivars is the most reliable and economical solution for sustainable wheat production as well as for long-term biosafety of our agriculture system.Modern wheat cultivars are established through negative selection and intensive breeding among high yielding cultivars. This process has resulted in narrowing the genetic diversity of our breeding material in general and elimination of vital genes/alleles for plant protection against diverse diseases in particular. On the other hand, fungal pathogen populations are multiplying and continuously evolving through frequent virulent changes through the co...

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Section: Methodsmentioning

confidence: 99%

“…The primary wheat progenitors believe to offer natural defence against many fungal diseases (Naz et al. , ). However, it is not easy to utilize these genetic resources in resistance breeding because of the linkage drag of wild alleles on the yield and quality parameters.…”

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Genetic analysis and detection of new QTL alleles for Septoria tritici blotch resistance using two advanced backcross wheat populations

et al. 2015

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“…Another QTL named QYr.cau-1BS, explaining about 10% of the phenotypic variation, was located in the region gwm374 -gwm264 -barc194 near the distal region far away from YrH62 (Quan et al 2013). In the near centromere region, Naz et al (2012) also reported a major effective QTL for stripe rust seedling resistance, QYrs.Z86-1B, originated from T. turgidum subsp. dicoccoides and it was finally considered as Yr15.…”

Section: Discussionmentioning

confidence: 99%

Combining Single Nucleotide Polymorphism Genotyping Array with Bulked Segregant Analysis to Map a Gene Controlling Adult Plant Resistance to Stripe Rust in Wheat Line 03031-1-5 H62

Wang

et al. 2018

Phytopathology®

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Stripe rust, caused by Puccinia striiformis f. sp. tritici, is one of the most devastating diseases of wheat worldwide. Growing resistant cultivars is considered the best approach to manage this disease. In order to identify the resistance gene(s) in wheat line 03031-1-5 H62, which displayed high resistance to stripe rust at adult plant stage, a cross was made between 03031-1-5 H62 and susceptible cultivar Avocet S. The mapping population was tested with Chinese P. striiformis f. sp. tritici race CYR32 through artificial inoculation in a field in Yangling, Shaanxi Province and under natural infection in Tianshui, Gansu Province. The segregation ratios indicated that the resistance was conferred by a single dominant gene, temporarily designated as YrH62. A combination of bulked segregant analysis (BSA) with wheat 90K single nucleotide polymorphism (SNP) array was used to identify molecular markers linked to YrH62. A total of 376 polymorphic SNP loci identified from the BSA analysis were located on chromosome 1B, from which 35 kompetitive allele-specific PCR (KASP) markers selected together with 84 simple sequence repeat (SSR) markers on 1B were used to screen polymorphism and a chromosome region associated with rust resistance was identified. To saturate the chromosomal region covering the YrH62 locus, a 660K SNP array was used to identify more SNP markers. To develop tightly linked markers for marker-assisted selection of YrH62 in wheat breeding, 18 SNPs were converted into KASP markers. A final linkage map consisting of 15 KASP and 3 SSR markers was constructed with KASP markers AX-109352427 and AX-109862469 flanking the YrH62 locus in a 1.0 cM interval. YrH62 explained 63.8 and 69.3% of the phenotypic variation for disease severity and infection type, respectively. YrH62 was located near the centromeric region of chromosome 1BS based on the positions of the SSR markers in 1B deletion bins. Based on the origin, responses to P. striiformis f. sp. tritici races, and marker distances, YrH62 is likely different from the other reported stripe rust resistance genes/quantitative trait loci on 1B. The gene and tightly linked KASP markers will be useful for breeding wheat cultivars with resistance to stripe rust.

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“…SSR primers were used to identify molecular markers flanking Yrxy2 , whereas for Yrxy1 RGAP and SSR markers both were used ( Zhou et al, 2011 ). Naz et al (2012) done QTL analysis by using a genetic map based on 118 SSR markers in 150 back cross lines of German wheat cultivars Zentos and Syn86L. To identify genes for stripe rust resistance in 179 F 2 population of Wuhan 2/Mingxian 169 cross against races CYR30 and CYR31 using RGAP and SSR markers ( Zhou et al, 2014b ).…”

Section: Yellow Rust or Stripe Rustmentioning

confidence: 99%

Recent trends and perspectives of molecular markers against fungal diseases in wheat

et al. 2015

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Wheat accounts for 19% of the total production of major cereal crops in the world. In view of ever increasing population and demand for global food production, there is an imperative need of 40–60% increase in wheat production to meet the requirement of developing world in coming 40 years. However, both biotic and abiotic stresses are major hurdles for attaining the goal. Among the most important diseases in wheat, fungal diseases pose serious threat for widening the gap between actual and attainable yield. Fungal disease management, mainly, depends on the pathogen detection, genetic and pathological variability in population, development of resistant cultivars and deployment of effective resistant genes in different epidemiological regions. Wheat protection and breeding of resistant cultivars using conventional methods are time-consuming, intricate and slow processes. Molecular markers offer an excellent alternative in development of improved disease resistant cultivars that would lead to increase in crop yield. They are employed for tagging the important disease resistance genes and provide valuable assistance in increasing selection efficiency for valuable traits via marker assisted selection (MAS). Plant breeding strategies with known molecular markers for resistance and functional genomics enable a breeder for developing resistant cultivars of wheat against different fungal diseases.

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Advanced backcross quantitative trait locus analysis in winter wheat: Dissection of stripe rust seedling resistance and identification of favorable exotic alleles originated from a primary hexaploid wheat (Triticum turgidum ssp. dicoccoides × Aegilops tauschii)

Cited by 13 publications

References 44 publications

Genetic analysis and detection of new QTL alleles for Septoria tritici blotch resistance using two advanced backcross wheat populations

Genetic analysis and detection of new QTL alleles for Septoria tritici blotch resistance using two advanced backcross wheat populations

Combining Single Nucleotide Polymorphism Genotyping Array with Bulked Segregant Analysis to Map a Gene Controlling Adult Plant Resistance to Stripe Rust in Wheat Line 03031-1-5 H62

Recent trends and perspectives of molecular markers against fungal diseases in wheat

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