Advanced backcross QTL analysis in progenies derived from a cross between a German elite winter wheat variety and a synthetic wheat (Triticum aestivumL.)

Abstract

We report here the second advanced backcross quantitative trait locus (AB-QTL) analysis carried out in winter wheat. Seven agronomic traits were studied in a BC2F1 population derived from a cross between the German winter wheat variety Flair and the synthetic wheat line XX86 developed in Japan. We selected 111 BC2F1 lines and genotyped these with 197 microsatellite markers. Field data for seven agronomic traits were collected from corresponding BC2F3 families that were grown at up to six locations in Germany. QTL analyses for yield and yield components were performed using single-marker regression and interval mapping. A total of 57 putative QTLs derived from XX86 were detected, of which 24 (42.1%) were found to have a positive effect from the synthetic wheat XX86. These favourable QQTLs were mainly associated with thousand-grain weight and grain weight per ear. Many QTLs for correlated traits were mapped in similar chromosomal regions. The AB-QTL data obtained in the present study are discussed and compared with results from previous QTL analyses.

Molecular genetic analysis of five spike-related traits in wheat using RIL and immortalized F2 populations

“…These results demonstrated that multiple loci with unequal eVects aVect yield traits and the expression of some of these loci has great environmental dependence. Through marker-based genotyping, chromosomal segments governing agronomical traits including yield traits have been identiWed in introgression lines (Huang et al 2004;Liu et al 2006;Narasimhamoorthy et al 2006). Although conventional quantitative genetic analysis has shown that additive gene action predominates in the genetic control of yield traits, dominance and epistasis eVects could not be ignored (Walton 1972;Gill et al 1977;Nanda et al 1981;Mckendry et al 1988;Goldringer et al 1997;Sharma et al 2003), which, however, have rarely been investigated in QTL mapping studies because of the genetic structure limitation of the double haploid populations or recombinant inbred line (RIL) populations used.…”

Verification of QTL controlling root aerenchyma formation in a maize * teosinte "Zea nicaraguensis" advanced backcross population

“…Accepted May 9, 2008 transfer of valuable QTL from donor to elite lines. AB-QTL mapping has been used in studies of tomato , Fulton et al 1997, Frary et al 2004, rice (Xiao et al 1998, Yamamoto et al 1998, wheat (Huang et al 2004) and barley (Pillen et al 2003). This method, using a BC 2 or BC 3 population of an interspecific cross, is a useful breeding strategy because once the QTLs are identified, it will be possible to develop near-isogenic lines or a series of chromosome segment substitution lines with a few additional backcrossing and selfing.…”

QTL Positioning of Thousand Wheat Grain Weight in Qaidam Basin

“…In the aspect of TGW, Borner took use of an ITMI population and obtained 3 stable QTL loci in 3AS, 5AL and 6BS [11], though they failed to be testified in the research of this paper. But in the QTL analysis of genealogy BC2F3 resulting from cross-breeding between female parent W7984 and "Prinz", Huang and the like acquired QTgw.ipk-4D, QTgw.ipk-5B, QTgw.ipk-7A, QTgw.ipk-7B.1, QTgw.ipk-7B.2, QTgw.ipk-7D [13]. Among these loci, chromosomes QTgw.…”

“…On the other hand, two parents tetraploid wheat and diploid Aegilops of artificially synthesized wheat carried disease-resistance gene and gene increasing yield [14]. When gene of artificially synthesized hexaploid wheat was introduced in common wheat, wheat quality and quantity was improved [13] [15]- [18]. In this study, parent Opata55 of ITMI population was a common Mexican wheat variety bred by CIMMYT and parent W7984 (M6) was an artificially synthesized variety cross-bred between Altar 84 (T. durum, AABB) and TA 2465 (Aegilops tauschii, DD) with chromosome doubling [19].…”