Advanced analytical methodologies in Alzheimer’s disease drug discovery

Simone, Angela De; Naldi, Marina; Tedesco, Daniele; Bartolini, Manuela; Davani, Lara; Andrisano, Vincenza

doi:10.1016/j.jpba.2019.112899

Cited by 17 publications

(9 citation statements)

References 260 publications

(257 reference statements)

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“…Several enzymes are targets to manage several diseases, and thus, the symptoms could be decreased by inhibiting these enzymes. Due to the side effects of synthetic compounds, researchers tend to focus on natural sources for the development of novel inhibitors with lower side effects [ 34 – 37 ]. Hence, we investigated the enzyme inhibitory effects of Sarcocapnos species against cholinesterases (AChE and BChE), tyrosinase, amylase, and glucosidase.…”

Section: Resultsmentioning

confidence: 99%

Study on Three Sarcocapnos Species as Potential Sources of Bioactive Compounds: Relation between Phenolic Content and Bioactivity by Multivariate Analysis

Fernández-Poyatos

Zengin

Salazar-Mendías

et al. 2020

Journal of Analytical Methods in Chemistry

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In this work, we report the phenolic composition and bioactivity of the aerial parts of three species of Sarcocapnos (S. enneaphylla, S. pulcherrima, and S. saetabensis) to study their potential as sources of bioactive compounds to revalorize them and contribute to the conservation of these plant species. Samples were collected in different locations in the province of Jaén (southeast of Spain), and qualitative and quantitative analyses of phenolic compounds were performed by high-performance liquid chromatography with diode array and mass spectrometry detection. S. enneaphylla presented the highest concentration of phenolic compounds (58 mg/g DE). The most abundant compound in S. enneaphylla and S. saetabensis was rutin (35 mg/g DE and 11.7 mg/g DE, respectively), whereas isorhamnetin-O-rutinoside was dominant in S. pulcherrima (11.5 mg/g DE). Several assays were performed to evaluate the potential bioactivity of the three species of Sarcocapnos. These assays included antioxidant and radical scavenging (ABTS and DPPH), reducing power (CUPRAC and FRAP), phosphomolybdenum and metal chelating, and enzyme inhibitory activity (acetylcholinesterase, amylase, butyrylcholinesterase, glucosidase, and tyrosinase). In general, all methanolic extracts presented the highest phenolic and flavonoid contents, as well as the highest radical scavenging, antioxidant, and enzyme inhibitory properties. This relationship between phenolics and bioactivity was confirmed by multivariate analysis.

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Section: Resultsmentioning

confidence: 99%

Study on Three Sarcocapnos Species as Potential Sources of Bioactive Compounds: Relation between Phenolic Content and Bioactivity by Multivariate Analysis

Fernández-Poyatos

Zengin

Salazar-Mendías

et al. 2020

Journal of Analytical Methods in Chemistry

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“…Altogether, this combination of optical and biological properties engenders a drug discovery protocol capable of accelerated evaluation of drug candidates in complex media approaching that of the cellular environments of target organs. Until now, it was nearly impossible to realize this capability with other peptide fibrillation protocols and optical methods, including traditional CD spectroscopy (43,44).…”

Section: Attractions Between Peptides (Figs S16 and S17)mentioning

confidence: 99%

Enhanced optical asymmetry in supramolecular chiroplasmonic assemblies with long-range order

Lü

Xue

Bernardino

et al. 2021

Science

192

215

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Chiral assemblies of plasmonic nanoparticles are known for strong circular dichroism but not for high optical asymmetry, which is limited by the unfavorable combination of electrical and magnetic field components compounded by strong scattering. Here we show that these limitations can be overcome by long-range organization of nanoparticles similar to liquid crystals found in helical assemblies of gold nanorods with human islet amyloid polypeptide. Strong polarization-dependent spectral shift and reduced scattering of energy states with antiparallel orientation of dipoles activated in assembled helices increase optical asymmetry g-factors by more than 4600 times. The liquid crystal-like color variations and nanorods-accelerated fibrillation enable drug screening in complex biological media. Improvement of long-range order also provides structural guidance for the design of materials with high optical asymmetry.

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“…2 Traditional biophysical methods for determining drug actions are based on inferences from bulk measurements of purified proteins or cells, including fluorescence resonance energy transfer (FRET), surface plasmon resonance (SPR), isothermal titration calorimetry (ITC), affinity selection mass spectrometry (AS-MS), and nuclear magnetic resonance (NMR) (reviewed in ref. [3][4][5]. Nevertheless, these approaches alone may not be sufficient to understand more detailed drug-acting mechanisms.…”

Section: Introductionmentioning

confidence: 99%

Novel perspective for protein–drug interaction analysis: atomic force microscope

Wang

2023

Analyst

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Advanced analytical methodologies in Alzheimer’s disease drug discovery

Cited by 17 publications

References 260 publications

Study on Three Sarcocapnos Species as Potential Sources of Bioactive Compounds: Relation between Phenolic Content and Bioactivity by Multivariate Analysis

Study on Three Sarcocapnos Species as Potential Sources of Bioactive Compounds: Relation between Phenolic Content and Bioactivity by Multivariate Analysis

Enhanced optical asymmetry in supramolecular chiroplasmonic assemblies with long-range order

Novel perspective for protein–drug interaction analysis: atomic force microscope

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