Adult toxicity and dominant lethals induced by ozone at specific stages in spermatogenesis in drosophila virilis

Abstract

Ozone, a highly reactive three-atom allotrope of oxygen, is formed in high concentrations in various kinds of microenvironments, including electron microscopes, office copy machines, and X-ray devices. Mutagenicity and action modus operandi of ozone suggest its radiomimetic property. In order to test for ozone-induced dominant lethals in Drosophila virilis, we determined that four-hr and longer exposures to 30 +/- 2 ppm ozone were lethal on or before day 25 postexposure. Accordingly, we used a three-hr ozone exposure to investigate dominant lethals induced at specific spermatogenic stages. Ozone increased the percentage of dominant lethals in the stages of spermatogenesis which were investigated. Under the conditions of this experiment, the sperm-sperm bundle stage showed the greatest percentage of dominant lethals induced by ozone. Meiotic cells were the least sensitive to ozone mutagenicity. The induction of dominant lethals is discussed on the basis of chemical reactions from the molecular decomposition of water by radiations and/or ozone. Alternative explanations are presented due to the definition of induced dominant lethals and to associated modifications from ozone exposure.

An Ozonolysis Based Method and Applications for the Non-Lethal Modification of Insect Cuticular Hydrocarbons

“…This is counter-intuitive to the reduction of the 7-tricosene (an anti-aphrodisiac) after ozonolysis but may be explained by the interaction of ozone with other insect tissues. For example, dominant lethal chemicals have been shown to be produced after ozone exposure, which cause mutagenicity and a reduced reproductive potential in D. virilis (Erdman and Hernandez 1982).…”

“…CLD marks differences between "Treatment -Hours" means based on an ANOVA p-values. Disparate letters only signify differences within a sex and age.CUTICULAR COMPOUND AMOUNT (NG) OF D. SUZUKIIAT 1,12, 36 AND H AFTER TREATMENT…”