Adult Stem Cells in Teeth

Mushegyan, Vagan; Horst, Orapin V.; Klein, Ophir D.

doi:10.1007/978-1-4614-9569-7_8

Cited by 2 publications

(2 citation statements)

References 84 publications

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“…Morfologi sel yang menyerupai sel fibroblas ini merupakan ciri utama untuk sel stem mesenkima seperti yang telah dinyatakan oleh International Society of Cellular Therapy (ISCT). Ciri morfologi ini juga turut ditunjukkan oleh BMSC semasa pengkulturan in vitro (Dominici et al 2006;Miura et al 2003;Mushegyan et al 2014).…”

Section: Pengasaian Alkali Fosfatase Bagi Sel Osteoblasunclassified

Pencirian dan Pembezaan Osteogenik Sel Stem Pulpa Gigi Kekal dan Gigi Desiduos Manusia

Yazid¹,

Luchman²,

Wahab³

et al. 2019

JSM

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Sel stem pulpa gigi daripada gigi kekal (DPSC) dan gigi desiduos (SHED) merupakan sel stem mesenkima dewasa yang mudah diperoleh dan berpotensi di dalam terapi sel. Pengasingan sel ini melibatkan pendekatan yang kurang invasif. Pencirian dan potensi pembezaan osteogenik bagi DPSC dan SHED adalah penting untuk memanipulasi sel ini bagi tujuan perubatan regeneratif. Tujuan kajian ini adalah untuk pencirian dan pengenalpastian potensi osteogenik bagi sel stem dewasa yang diasingkan daripada tisu pulpa gigi kekal dan gigi desiduos manusia. Pulpa gigi dipencilkan daripada gigi kekal dan gigi desiduos manusia menggunakan kaedah pencernaan enzim dan seterusnya dikulturkan sehingga pasaj 3. Morfologi sel dikenal pasti menggunakan perisian CellB. Kadar proliferasi DPSC dan SHED pula ditentukan menggunakan pengasaian tiazolilbiru tetrazolium bromida (MTT). Sementara itu, potensi pembezaan osteoblas bagi DPSC dan SHED ditentukan menggunakan analisis biokimia. Hasilnya, kedua-dua jenis sel ini menunjukkan morfologi menyerupai sel fibroblas pada pasaj 3. Kadar proliferasi SHED pula menunjukkan secara signifikan (p<0.05) ia jauh lebih tinggi berbanding DPSC. Kajian ini turut menunjukkan bahawa aktiviti alkali fosfatase (ALP) bagi SHED adalah secara signifikannya lebih tinggi berbanding dengan DPSC (p<0.05) apabila dikulturkan di dalam medium pembezaan osteoblas. Kesimpulannya, DPSC dan SHED mampu untuk membahagi dan membeza kepada osteoblas. Walau bagaimanapun, SHED telah menunjukkan kadar proliferasi serta potensi osteogenik yang lebih tinggi berbanding DPSC.

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Section: Pengasaian Alkali Fosfatase Bagi Sel Osteoblasunclassified

Pencirian dan Pembezaan Osteogenik Sel Stem Pulpa Gigi Kekal dan Gigi Desiduos Manusia

Yazid¹,

Luchman²,

Wahab³

et al. 2019

JSM

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“…Primary teeth are completed in 5- to 6-year-old children, and these teeth are lost around age 12, whereas permanent teeth begin to erupt at age 6 and can continue erupting until age 17–25 (Volponi et al, 2010 ; Mushegyan et al, 2014 ). Inside each primary or permanent tooth is dental pulp (DP), which is an innervated, highly vascularized soft tissue that provides vitality to the tooth.…”

Section: From Tooth To Dental Tissue-derived Stem Cellsmentioning

confidence: 99%

Stem Cells from Dental Pulp: What Epigenetics Can Do with Your Tooth

et al. 2017

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Adult stem cells have attracted scientific attention because they are able to self-renew and differentiate into several specialized cell types. In this context, human dental tissue-derived mesenchymal stem cells (hDT-MSCs) have emerged as a possible solution for repairing or regenerating damaged tissues. These cells can be isolated from primary teeth that are naturally replaced, third molars, or other dental tissues and exhibit self-renewal, a high proliferative rate and a great multilineage potential. However, the cellular and molecular mechanisms that determine lineage specification are still largely unknown. It is known that a change in cell fate requires the deletion of existing transcriptional programs, followed by the establishment of a new developmental program to give rise to a new cell lineage. Increasing evidence indicates that chromatin structure conformation can influence cell fate. In this way, reversible chemical modifications at the DNA or histone level, and combinations thereof can activate or inactivate cell-type-specific gene sequences, giving rise to an alternative cell fates. On the other hand, miRNAs are starting to emerge as a possible player in establishing particular somatic lineages. In this review, we discuss two new and promising research fields in medicine and biology, epigenetics and stem cells, by summarizing the properties of hDT-MSCs and highlighting the recent findings on epigenetic contributions to the regulation of cellular differentiation.

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Adult Stem Cells in Teeth

Cited by 2 publications

References 84 publications

Pencirian dan Pembezaan Osteogenik Sel Stem Pulpa Gigi Kekal dan Gigi Desiduos Manusia

Pencirian dan Pembezaan Osteogenik Sel Stem Pulpa Gigi Kekal dan Gigi Desiduos Manusia

Stem Cells from Dental Pulp: What Epigenetics Can Do with Your Tooth

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