Adult stem cell therapy: Dream or reality?

Moraleda, José Marı́a; Blanquer, Miguel; Bleda, Patricia; Iniesta, Paqui; Ruiz, Francisco; Bonilla, Sonia; Cabanes, Carmen; Tabares, Lucı́a

doi:10.1016/j.trim.2006.09.030

Cited by 37 publications

(26 citation statements)

References 12 publications

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“…Reverse transcription was performed with SuperScript III (Invitrogen Life Technologies) and an Oligo (dT) [12][13][14][15][16][17][18] primer. Four micrograms of RNA were added into a final volume of 21-mL solution containing 10 mM deoxynucleotide triphosphate mix, 10· RT buffer, 25 mM MgCl 2 , 0.1 M dithiothreitol, RNase inhibitor, and RNase H. Six micrograms of RT product were used for the PCR amplification in a final volume of 50 mL containing 2.5 mM deoxynucleotide triphosphate, 25 mM MgCl 2 , upstream or downstream primers (nestin and GDNF), and Taq DNA polymerase (Invitrogen Life Technologies).…”

Section: Rna Extraction and Semiquantitative Polymerase Chain Reactiomentioning

confidence: 99%

“…It is hoped that these undifferentiated cells will provide an inexhaustible source of neurons and glia for therapies aimed at cell replacement or neuroprotection in disorders affecting the central nervous system. The transplantation of stem cells could promote functional recovery by reconstituting damaged nerve tracts, remyelinating axons, and increasing plasticity or axon regeneration (12). For instance, locomotor improvements have been demonstrated in spinal cord-injured mice and primates (13)(14)(15).…”

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confidence: 99%

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A Novel Cell-Based Therapy for Contusion Spinal Cord Injury Using GDNF-Delivering NIH3T3 Cells with Dual Reporter Genes Monitored by Molecular Imaging

Lo¹,

Hsu²,

et al. 2008

J Nucl Med

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This aim of our study was to evaluate a novel cell-based therapy for contusion spinal cord injury (SCI) using embryonic-derived NIH3T3 cells, which endogenously express glial cell line-derived neurotrophic factor (GDNF). Methods: Proliferation and differentiation of transplanted NIH3T3 cells and their anti-apoptotic effects were examined after their engraftment into the spinal cords of Long-Evans rats subjected to acute SCI at the T10 vertebral level by a New York University impactor device. NIH3T3 cells were initially engineered to contain dual reporter genes, namely thymidine kinase (T) and enhanced green fluorescence protein (G), for in vivo cell tracking by both nuclear and fluorescence imaging modalities. Results: Planar and fluorescence imaging demonstrated that transplanted NIH3T3-TG cells at the L1 vertebral level migrated 2 cm distal to the injury site as early as 2 h, and the signals persisted for 48 h after SCI. The expression of GDNF by NIH3T3-TG cells was then confirmed by immunohistochemical analysis both in vitro and in vivo. GDNFsecreting NIH3T3-TG transplant provided anti-apoptotic effects in the injured cord over the period of 3 wk. Finally, NIH3T3-TG cells cultured under neuronal differentiation medium exhibited both morphologic and genetic resemblance to neuronal cells. Conclusion: GDNF-secreting NIH3T3-TG cells in combination with molecular imaging could be a platform for developing therapeutic tools for acute SCI.

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Section: Rna Extraction and Semiquantitative Polymerase Chain Reactiomentioning

confidence: 99%

mentioning

confidence: 99%

A Novel Cell-Based Therapy for Contusion Spinal Cord Injury Using GDNF-Delivering NIH3T3 Cells with Dual Reporter Genes Monitored by Molecular Imaging

Lo¹,

Hsu²,

et al. 2008

J Nucl Med

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“…Bone marrow is a source of mesenchymal stromal stem cells (MSCs) which have demonstrated in vivo and in vitro ability to differentiate into osteoblasts and chondrocytes, thus providing tissue repair capacities [1,2]. Their functional properties have been confirmed in several studies using autologous human bone marrow mesenchymal stem cells ( h BMMSC) for bone repairing and tissue healing [3].…”

Section: Introductionmentioning

confidence: 99%

Preclinical Studies of the Biosafety and Efficacy of Human Bone Marrow Mesenchymal Stem Cells Pre-Seeded into β-TCP Scaffolds after Transplantation

et al. 2018

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Background: Cell-Based Therapies (CBT) constitute a valid procedure for increasing the quantity and quality of bone in areas with an inadequate bone volume. However, safety and efficacy should be investigated prior to clinical application. The objective of this study was to evaluate the biodistribution, safety and osteogenic capacity of bone marrow-derived human mesenchymal stem cells (hBMMSCs) pre-seeded into β-tricalcium phosphate (TCP) and implanted into NOD/SCID mice at subcutaneous and intramuscular sites. Methods: hBMMSCs were isolated, characterized and then cultured in vitro on a porous β-TCP scaffold. Cell viability and attachment were analyzed and then hBMMSCs seeded constructs were surgically placed at subcutaneous and intramuscular dorsal sites into NOD/SCID mice. Acute and subchronic toxicity, cell biodistribution and efficacy were investigated. Results: There were no deaths or adverse events in treated mice during the 48-hour observation period, and no toxic response was observed in mice. In the 12-week subchronic toxicity study, no mortalities, abnormal behavioral symptoms or clinical signs were observed in the saline control mice or the hBMMSCs/β-TCP groups. Finally, our results showed the bone-forming capacity of hBMMSCs/β-TCP since immunohistochemical expression of human osteocalcin was detected from week 7. Conclusions: These results show that transplantation of hBMMSCs/β-TCP in NOD/SCID mice are safe and effective, and might be applied to human bone diseases in future clinical trials.

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“…6,7 Stem cells are defined as clonogenic, self-renewing progenitor cells that can generate one or more specialized cell types. 8 MSCs are a population of multipotent progenitor cells, which are easily expanded in a culture and differentiate into cells with an osteogenic phenotype. They were originally isolated from adult bone marrow and subsequently from other tissues.…”

Section: Introductionmentioning

confidence: 99%

Synergistic effect of bone marrow-derived mesenchymal stem cells and platelet-rich plasma on bone regeneration of calvarial defects in rabbits

Yun

Yoo

Choi

et al. 2012

Tissue Eng Regen Med

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Bone tissue regeneration techniques include tissue engineering approaches which employ mesenchymal stem cells as an osteogenic agent for bone repair. Recent studies have demonstrated that tissue engineering scaffolds and growth factors can support cell proliferation, bone formation, and bone tissue repair in lost bone tissue. Furthermore, many studies have suggested that platelet-rich plasma (PRP) can improve bone regeneration due to the numerous growth factors that it contains. This study was performed to investigate the influence of bone marrow-derived mesenchymal stem cells (BMMSCs) and PRP on bone regeneration of calvarial defects in rabbits. Hydroxyapatite (HA) was used as a scaffold for bone regeneration. There were three groups in this experiment: 1) HA loaded with BMMSCs (HS group), 2) HA loaded with PRP (HP group), and 3) HA loaded with BMMSCs and PRP (HSP group). Two circular bony defects (6 mm in diameter) were made in rabbit calvaria using a trephine bur. BMMSCs and PRP with a HA scaffold (diameter 5.5 mm, height 3 mm) were applied to each defect. The animals were sacrificed after 2 weeks, 4 weeks and 8 weeks. The level of their ability of osteogenesis was evaluated through histological and histomorphometric analyses. High-quality bone regeneration was observed in the HSP group. The percentage of new bone area around the scaffolds was higher in the HSP group than it was in the other groups (HS and HP group), especially at 8 weeks (HS, 72.5±15 %; HP, 85.8±14 %; HSP, 98.8±2.5%). In addition, the level of bone maturation was higher in the HSP group than in the other groups. The results of this study show that PRP has a positive effect on bone generation. HA with a combination of BMMSCs and PRP can enhance bone regeneration. In addition, the growth factor capacity of PRP may affect the differentiation of BMMSCs and promote bone formation.

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Adult stem cell therapy: Dream or reality?

Cited by 37 publications

References 12 publications

A Novel Cell-Based Therapy for Contusion Spinal Cord Injury Using GDNF-Delivering NIH3T3 Cells with Dual Reporter Genes Monitored by Molecular Imaging

A Novel Cell-Based Therapy for Contusion Spinal Cord Injury Using GDNF-Delivering NIH3T3 Cells with Dual Reporter Genes Monitored by Molecular Imaging

Preclinical Studies of the Biosafety and Efficacy of Human Bone Marrow Mesenchymal Stem Cells Pre-Seeded into β-TCP Scaffolds after Transplantation

Synergistic effect of bone marrow-derived mesenchymal stem cells and platelet-rich plasma on bone regeneration of calvarial defects in rabbits

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