Adult rat bone marrow stromal cells differentiate into Schwann cell-like cells in vitro

Lin, Wei; Xue, Cong; Wang, Xiao Dong; Liu, Jie; Gu, Xiao Song

doi:10.1007/s11626-007-9064-y

Cited by 52 publications

(30 citation statements)

References 39 publications

(38 reference statements)

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“…Chitosan is shown to be a favorable conduit to fill with differentiated Schwann cells for transplantation [74]. Histologically, transplanted cells were confirmed to form myelin and express myelin-related proteins such as MAG, myelinbasic protein, P0 or peripheral myelin protein 22 (PMP22) [22,65,75,76].…”

Section: • Bmscsmentioning

confidence: 99%

Mesenchymal Stem Cells and Umbilical Cord as Sources for Schwann Cell Differentiation: their Potential in Peripheral Nerve Repair

Kuroda¹

2011

TOTERMJ

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Schwann cells are important components of the peripheral glia that form myelin, serving as the microenvironment of nerve fibers in the peripheral nervous system (PNS). Damage to the PNS induces the differentiation and activation of Schwann cells to produce factors that strongly promote axonal regrowth, and subsequently contribute to remyelination, which is crucial for the recovery of function. Although the collection and transplantation of native Schwann cells are effective for the treatment of neural diseases, isolation of Schwann cells results in new damage to other peripheral nerve segments and causes undesirable iatrogenic injury in the donor. Furthermore, the expansion of native Schwann cells to obtain a sufficient number of cells for clinical application within a reasonable period is technically difficult. Therefore, a method to induce easily accessible and highly proliferative cells to differentiate into cells with Schwann cell properties would be very practical and is highly desirable. Recently, regenerative medicine has focused on mesenchymal stem cells because they are easily accessible from various kinds of mesenchymal tissues such as the umbilical cord, bone marrow, and fat tissue. Mesenchymal stem cells are highly proliferative and it is easy to obtain an adequate number of cells. Notably, while mesenchymal stem cells are mesodermal lineage cells, they have an ability to cross oligolineage boundaries previously thought uncrossable to achieve transdifferentiation. In this review, we focus on the potential of mesenchymal stem cells, particularly umbilical cord-derived mesenchymal stem cells, to differentiate into functional Schwann cells, and discuss the prospective clinical application of these cells to PNS regeneration.

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Section: • Bmscsmentioning

confidence: 99%

Mesenchymal Stem Cells and Umbilical Cord as Sources for Schwann Cell Differentiation: their Potential in Peripheral Nerve Repair

Kuroda¹

2011

TOTERMJ

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“…This induction system resulted in a rate of 42.9 ± 3.3% S100-positive cells. Lin et al [19] reported pre-induction using b-mercaptoethanol, and then supplementing with some related factors could result in a rate of 25% S100-positive cells. We adopted a two-step induction protocol of the HUMSCs: at first, the long spindle-shaped HUMSCs were induced into neural stem cell like structures (neurospheres), and then some factors were added into the culture medium to further induce the neurospheres to differentiate into neuron-like cells bearing morphological, immunophenotypic and functional resemblances with Schwann-like cells, which avoid the harmfulness of b-mercaptoethanol to the researchers.…”

Section: Discussionmentioning

confidence: 99%

Human Umbilical Cord-Derived Schwann-Like Cell Transplantation Combined with Neurotrophin-3 Administration in Dyskinesia of Rats with Spinal Cord Injury

Guo

et al. 2011

Neurochem Res

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Mesenchymal stem cells are capable of differentiating into Schwann-like cells. In this study, we induced human umbilical-cord mesenchymal stem cells (HUMSCs) in vitro into neurospheres constituted by neural stem-like cells, and further into cells bearing strong morphological, phenotypic and functional resemblances with Schwann-like cells. These HUMSC-derived Schwann-like cells, after grafting into the injured area of the rats' spinal cord injury (SCI), showed a partial therapeutic effect in terms of improving the motor function. Neurotrophin-3 (NT-3) was reported to improve the local microenvironment of the grafted cells, and we, therefore, further tested the effect of Schwann-like cell grafting combined with NT-3 administration at the site of cell transplantation. The results showed that NT-3 administration significantly promoted the survival of the grafted cells in the host-injured area. Significant improvement in rats treated by Schwann-like cell grafting combined with NT-3 administration was demonstrated in the behavioral test as compared with that in animal models received the cell grafting only. These results suggest that transplantation of the Schwann-like cells combined with NT-3 administration may represent a new strategy of stem cell therapy for spinal cord injury.

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“…These stem cells can be induced to differentiate into SClike cells by using platelet-derived growth factor, basic fibroblast growth factor, and glial growth factor-2 (Keilhoff and Fansa 2011). Studies have demonstrated that more than 50 % of cells differentiated with this protocol (Dezawa et al 2001) typically exhibit SC-like phenotypes and, more importantly, that they successfully support nerve regeneration (Caddick et al 2006;Keilhoff et al 2006a, b;Lin et al 2008). Specifically, differentiated BMSCs exert a significant neurotrophic effect via secretion of factors that support neuronal survival and growth affecting both outgrowing fibers and nerve cell bodies in a dorsal root ganglion neuronal functional assay (Caddick et al 2006;Mahay et al 2008).…”

Section: Mesenchymal Stem Cellsmentioning

confidence: 99%

Stem Cell-Based Approaches to Improve Nerve Regeneration: Potential Implications for Reconstructive Transplantation?

Khalifian

Sarhane

Tammia

et al. 2014

Arch. Immunol. Ther. Exp.

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Reconstructive transplantation has become a viable option to restore form and function after devastating tissue loss. Functional recovery is a key determinant of overall success and critically depends on the quality and pace of nerve regeneration. Several molecular and cell-based therapies have been postulated and tested in pre-clinical animal models to enhance nerve regeneration. Schwann cells remain the mainstay of research focus providing neurotrophic support and signaling cues for regenerating axons. Alternative cell sources such as mesenchymal stem cells and adipose-derived stromal cells have also been tested in pre-clinical animal models and in clinical trials due to their relative ease of harvest, rapid expansion in vitro, minimal immunogenicity, and capacity to integrate and survive within host tissues, thereby overcoming many of the challenges faced by culturing of human Schwann cells and nerve allografting. Induced pluripotent stem cell-derived Schwann cells are of particular interest since they can provide abundant, patient-specific autologous Schwann cells. The majority of experimental evidence on cell-based therapies, however, has been generated using stem cell-seeded nerve guides that were developed to enhance nerve regeneration across "gaps" in neural repair. Although primary end-to-end repair is the preferred method of neurorrhaphy in reconstructive transplantation, mechanistic studies elucidating the principles of cell-based therapies from nerve guidance conduits will form the foundation of further research employing stem cells in end-to-end repair of donor and recipient nerves. This review presents key components of nerve regeneration in reconstructive transplantation and highlights the pre-clinical studies that utilize stem cells to enhance nerve regeneration.

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Adult rat bone marrow stromal cells differentiate into Schwann cell-like cells in vitro

Cited by 52 publications

References 39 publications

Mesenchymal Stem Cells and Umbilical Cord as Sources for Schwann Cell Differentiation: their Potential in Peripheral Nerve Repair

Mesenchymal Stem Cells and Umbilical Cord as Sources for Schwann Cell Differentiation: their Potential in Peripheral Nerve Repair

Human Umbilical Cord-Derived Schwann-Like Cell Transplantation Combined with Neurotrophin-3 Administration in Dyskinesia of Rats with Spinal Cord Injury

Stem Cell-Based Approaches to Improve Nerve Regeneration: Potential Implications for Reconstructive Transplantation?

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