Adult pancreatic stem/progenitor cells spontaneously differentiate in vitro into multiple cell lineages and form teratoma-like structures

Kruse, Charli; Kajahn, Jennifer; Petschnik, Anna Emilia; Maass, Antje; Klink, E.C.; Rapoport, Daniel H.; Wedel, Thilo

doi:10.1016/j.aanat.2006.07.012

Cited by 75 publications

(58 citation statements)

References 35 publications

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“…A population of stem cells that express markers of ESC/epiblast/PGC cells was recently described in several nonhematopoietic organs, for example, in epidermis (Dyce et al, 2004(Dyce et al, , 2006Yu et al, 2006), bronchial epithelium (Ling et al, 2006), myocardium, pancreas, (Kruse et al, 2006;Danner et al, 2007), testis (Kanatsu-Shinohara et al, 2004;Guan et al, 2006), retina (Koso et al, 2006), and amniotic fluid (De Coppi et al, 2007). This finding supports a concept of developmental deposition of Oct-4 ϩ EPSC in developing organs.…”

Section: Oct-4 ؉ Stem Cells In Other Tissuessupporting

confidence: 77%

Bone marrow‐derived very small embryonic‐like stem cells: Their developmental origin and biological significance

Kucia

Wan

Ratajczak

2007

Developmental Dynamics

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Data from our and other laboratories provide evidence that bone marrow (BM) contains a population of stem cells that expresses early developmental markers such as (1) stage-specific embryonic antigen (SSEA) and (2) transcription factors Oct-4 and Nanog. These are the markers characteristic for embryonic stem cells, epiblast stem cells, and primordial germ cells (PGC). The presence of these stem cells in adult BM supports the concept that this organ contains some population of pluripotent stem cells that is deposited in embryogenesis during early gastrulation. We hypothesize that these cells could be direct descendants of the germ lineage that, to pass genes on to the next generations, has to create soma and, thus, becomes a "mother lineage" for all somatic cell lineages present in the adult body. Germ potential is established after conception in totipotent zygotes and retained in blastomeres of morula, cells from the inner cell mass of blastocyst, epiblast, and population of PGC. We will present a concept that SSEA ؉ Oct-4 ؉ Nanog ؉ cells identified in BM could be descendants of epiblast cells as well as some rare migrating astray PGC. Developmental Dynamics 236:3309 -3320, 2007.

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Section: Oct-4 ؉ Stem Cells In Other Tissuessupporting

confidence: 77%

Bone marrow‐derived very small embryonic‐like stem cells: Their developmental origin and biological significance

Kucia

Wan

Ratajczak

2007

Developmental Dynamics

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“…n the past decades, the potential of stem cells to differentiate into female germ cells has been demonstrated and reported for embryonic stem cells (reviewed in Ko et al [1]) and somatic stem cells [2][3][4][5][6]. In an ovarian follicle, the oocyte is surrounded by cumulus cells and the follicular wall is composed of granulosa and theca cells.…”

mentioning

confidence: 99%

Ovarian-Cell-Like Cells from Skin Stem Cells Restored Estradiol Production and Estrus Cycling in Ovariectomized Mice

Park

Pan²,

Toms³

et al. 2014

Stem Cells and Development

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Reduction of estradiol production and high serum concentrations of follicular stimulating hormone (FSH) are endocrine disorders associated with premature ovarian failure. Here, we report that transplantation of ovarianlike cells differentiated from stem cells restored endogenous serum estradiol levels. Stem cells were isolated from postnatal mouse skin and differentiated into ovarian-cell-like cells that are consistent with female germ, and ovarian follicle somatic cells. The ovarian-cell-like cells were transplanted into ovariectomized mice (Cell Trans), whereas control mice were subjected to bilateral ovariectomies without cell transplantation (OVX). Using vaginal cytology analysis, it was revealed that in 13 out of 19 Cell Trans mice, estrus cycles were restored around 8 weeks after cell transplantation and were maintained until 16 weeks post-transplantation, whereas in the OVX group, all mice were arrested at metestrus/diestrus of the estrus cycle. The uterine weight in the Cell Trans group was similar to sham operation mice (Sham OP), while severe uterine atrophy and a decreased uterine weight were observed in the OVX group. Histologically, ectopic follicle-like structures and blood vessels were found within and around the transplants. At 12-14 weeks after cell transplantation, mean serum estradiol level in Cell Trans mice (178.0 -35 pg/mL) was comparable to that of the Sham OP group (188.9 -29 pg/mL), whereas it was lower in the OVX group (59.0 -4 pg/mL). Serum FSH concentration increased in the OVX group (1.62 -0.32 ng/mL) compared with the Sham OP group (0.39 -0.34 ng/mL). Cell Trans mice had a similar FSH level (0.94 -0.23 ng/mL; P < 0.05) to Sham OP mice. Our results suggest that ovarian somatic cells differentiated from stem cells are functional in vivo. In addition to providing insights into the function of ovarian somatic cells derived from stem cells, our study may offer potential therapeutic means for patients with hypo-estradiol levels like those encountered in premature ovarian failure.

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“…Also the occurrence of single undifferentiated cells within well-differentiated cell clusters may cause problems since this can lead to unregulated growth such as the formation of teratomas [17][18][19][20].…”

Section: Biophotonicsmentioning

confidence: 99%

“…The stem cell lines hSGSC (human salivary gland stem cells), hPSC (human pancreatic stem cells), rPSC (rat pancreatic stem cells) were used [20][21]. Cells were cultured in Dulbecco's modified Eagle's medium (DMEM) (GIBCO, Germany) containing 4.5 g/L glucose, L-glutamine and supplemented with 10% fetal calf serum Gold (FCS-Gold) (PAA Laboratories, GmbH), 100 U/ml penicillin, and 100 mg/ml streptomycin.…”

Section: Cell Lines and Sample Preparationmentioning

confidence: 99%

Optical knock out of stem cells with extremely ultrashort femtosecond laser pulses

Uchugonova

Isemann²,

Gorjup

et al. 2008

Journal of Biophotonics

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Novel ultracompact multiphoton. sub-20 femtosecond near infrared 85MHz laser scanning microscopes and conventional 250 fs laser microscopes have been used to perform, high spatial resolution two-photon imaging of stem cell clusters as well, as selective-,int.l.lacellula,r nanoprocessing and knock out of living single stem cells within an 3D microenvironment without any collateral damage. Also lethal, cell exposure of large, parts of cell clusters was successfully probed while maintaining single cells of interest alive. The mew power could be kept in the milliwatt range for 3D nanoprocessing and even in the microwatt range for two-photon imabing. Ultracompact low power sub-20 fs laser systems may becone interesting tools for optical nanobiotechnology such as optical cleaning of stem clusters as well as optical transfection. [GRAPHICS] Optical knock out of a single human pancreatic stem cell without collateral damage

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Adult pancreatic stem/progenitor cells spontaneously differentiate in vitro into multiple cell lineages and form teratoma-like structures

Cited by 75 publications

References 35 publications

Bone marrow‐derived very small embryonic‐like stem cells: Their developmental origin and biological significance

Bone marrow‐derived very small embryonic‐like stem cells: Their developmental origin and biological significance

Ovarian-Cell-Like Cells from Skin Stem Cells Restored Estradiol Production and Estrus Cycling in Ovariectomized Mice

Optical knock out of stem cells with extremely ultrashort femtosecond laser pulses

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